Dual-signal, One-step Simultaneous Monitoring of Genetic Mutation in Multiple Gene Regions Using Fe3O4@Au and MOF. DOI
Songrong Ren, Xinqing Zhao, Songmei Geng

et al.

Talanta, Journal Year: 2024, Volume and Issue: 285, P. 127384 - 127384

Published: Dec. 14, 2024

Language: Английский

CRISPR integrated biosensors: A new paradigm for cancer detection DOI

Ashok Saini,

Neeraj Dilbaghi, Neelam Yadav

et al.

Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: 569, P. 120179 - 120179

Published: Feb. 1, 2025

Language: Английский

Citations

2
Signal-amplification strategy and advances in electrochemistry-based CRISPR/Cas12 biosensing DOI
Likun Pan, Weiwei Jiang, Fei Deng

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 161110 - 161110

Published: Feb. 1, 2025

Language: Английский

Citations

2
CRISPR/Cas12a regulated dual-channel ratiometric fluorescence biosensing for sensitive and accurate detection of kanamycin DOI
Xia Yi, Min‐Hsien Wu,

Xinyue Yuwen

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113581 - 113581

Published: April 1, 2025

Language: Английский

Citations

1
Catalytic hairpin-assembled and DNAzyme-driven DNA walker integrated hybridization chain reaction to construct a label-free electrochemical aptasensor for the detection of acetamiprid DOI
Xuemei Zhang,

Li Zhu,

Li Yang

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 418, P. 136253 - 136253

Published: July 4, 2024

Language: Английский

Citations

6
An Innovative and Efficient Fluorescent Detection Technique for Salmonella in Animal-Derived Foods Using the CRISPR/Cas12a-HCR System Combined with PCR/RAA DOI

Yuanshang Wang,

Pengfei Du,

Yanchun Shao

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(15), P. 8831 - 8839

Published: April 4, 2024

Here, we present a method for Salmonella detection using clustered regularly interspaced short palindromic repeats associated with the CRISPR-associated protein 12a-hybridization chain reaction (CRISPR/Cas12a-HCR) system combined polymerase reaction/recombinase-assisted amplification (PCR/RAA) technology. The approach relies on invA gene as biorecognition element and its through PCR RAA. In presence of target gene, Cas12a, guided by crRNA, recognizes cleaves product, initiating HCR. Fluorescently labeled single-stranded DNA (ssDNA) H1 H2 were introduced, concentration was determined based fluorescence intensity from triggered Both assays demonstrate high specificity, sensitivity, simplicity, rapidity. range 2 × 101–2 109 CFU/mL, an LOD 20 entire process enabled specific rapid within 85–105 min. Field-incurred spiked recovery tests conducted in mutton beef samples both assays, demonstrating satisfactory accuracy animal-derived foods. By combining CRISPR/Cas12a hybridization technology, this study presents sensitive that is crucial identifying pathogenic bacteria monitoring food safety.

Language: Английский

Citations

5
Dual electrochemical signal “signal-on-off” sensor based on CHA-Td-HCR and CRISPR-Cas12a for MUC1 detection DOI

Hehua Zhang,

Hongmin Gao,

Simin Liu

et al.

Talanta, Journal Year: 2024, Volume and Issue: 279, P. 126665 - 126665

Published: Aug. 3, 2024

Language: Английский

Citations

4
CRISPR/Cas12a-triggered ordered concatemeric DNA probes signal-on/off multifunctional analytical sensing system for ultrasensitive detection of thalassemia DOI
Peiyuan Li,

Yashu Wei,

Jinyue Shi

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 276, P. 133884 - 133884

Published: July 14, 2024

Language: Английский

Citations

3
A MXene-Au Nanosheets-based Fluorescent-SERS Dual-Mode Biosensor Integrated with CRISPR/Cas12a System for Endotoxin Detection DOI

Honglian Wu,

Ruolin Zhang,

Huang Lai

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: unknown, P. 137120 - 137120

Published: Dec. 1, 2024

Language: Английский

Citations

3
Biosensors for detection of hormones and growth factors DOI
Vahid Reza Askari, Mohammad Reza Fadaei, Vahid Tayebi Khorrami

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 181 - 213

Published: Jan. 1, 2025

Language: Английский

Citations

0
Exploring a New Ruthenium(II) Complex with High DNA Binding Ability as a Novel Efficient Luminescent Intercalation Agent to Construct a Label-Free ECL/PL Dual-Mode Biosensor DOI

Yaoyao Xu,

Rui Deng, Junli Jia

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Ruthenium(II) complexes with special ligands have been widely recognized in numerous fields and attributed to their outstanding DNA binding capacity. Hybridization chain reaction (HCR), as an enzyme-free amplification technique, forms long double-stranded (dsDNA) structures, which provides intercalation platform for these obtains effective enhancement of luminescent signals a significant extent enhances the sensitivity detection. Hence, Ru(dip)2(tpphz) [dip = 4,7-diphenyl-1,10-phenanthroline, tpphz tetrapyrido[3,2-a:2′,3′-c:3″,2″-h:2‴,3‴-j]phenazine] confirmed possess high capacity via UV–vis absorption spectroscopy AutoDock theoretical simulation calculations was synthesized luminescence probe. As proof concept, label-free ECL/PL dual-mode biosensor further constructed. In this design, magnetic silica spheres trigger were amplified by HCR hairpin DNA, forming large amounts dsDNA on surface, incorporated generate robust signals. Trigger cleaved owing activation Cas12a cleavage ability presence target, disappeared, reduced. The exhibited selectivity, LOD low 69 fM (S/N 3). results proved that has excellent ECL PL dual properties, huge potential establish biosensors simultaneously offers tremendous prospect anticancer, gene therapy, molecular probes beyond future.

Language: Английский

Citations

0