Precise diagnosis of tumor cells and hemocytes using ultrasensitive, stable, selective cuprous oxide composite SERS bioprobes assisted with high-efficiency separation microfluidic chips

Materials Horizons, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

In this study, we reported a flower-like Cu 2 O@Ag SERS substrate for distinguish three types of cancer cells from white blood by using machine learning-assisted LDA, after separating samples via microfluidic chip.

Язык: Английский

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Nanozyme-Catalyzed Colorimetric Microfluidic Immunosensor for the Filtration Enrichment and Ultrasensitive Detection of Salmonella typhimurium in Food Samples

Analytical Chemistry, Год журнала: 2025, Номер 97(12), С. 6454 - 6463

Опубликована: Март 5, 2025

Rapid screening of foodborne pathogens is crucial to prevent food poisoning. In this study, we proposed a nanozyme-catalyzed colorimetric microfluidic immunosensor (Nano-CMI) for the filtration enrichment and ultrasensitive detection Salmonella typhimurium in complex matrices. Gold-core porous platinum shell nanopompoms (Au@Pt nanopompoms) were synthesized with excellent peroxidase-like activity oxidize 3,3',5,5'-tetramethylbenzidine significant color change. The Au@Pt demonstrated large reaction area, superior catalytic property, good stability. chip used Nano-CMI was designed based on size disparities among S. typhi, nanopompoms, pore sizes filters I II. Thus, biosensor containing pretreatment, incubation, enrichment, four-in-one functions established performed under drive medical plastic syringe. This can accomplish typhi limit as low 9 cfu/mL within 20 min, which makes it suitable point-of-care testing. also possessed high specificity repeatability (RSD < 2.1%) thus be applied directly analysis real samples, suggesting its great potential practical application safety field.

Язык: Английский

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Effect of complex vortices generated by asymmetrically distributed induced charges on fluid flow and mass transfer in pressure driven micromixers

Chaos Solitons & Fractals, Год журнала: 2025, Номер 196, С. 116373 - 116373

Опубликована: Апрель 4, 2025

Язык: Английский

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Magnetophoresis-Enhanced Elasto-Inertial Migration of Microparticles and Cells in Microfluidics

Analytical Chemistry, Год журнала: 2024, Номер 96(9), С. 3925 - 3932

Опубликована: Фев. 12, 2024

Microfluidic particle and cell manipulation techniques possess many potentials for biomedicine healthcare. Many have been developed based on active (e.g., electrical, magnetic, acoustic, thermal) force fields passive hydrodynamic forces inertial elastic lift forces). However, a single or manipulating physics cannot always meet the demands, combining multiple becomes promising strategy to promote technique flexibility versatility. In this work, we explored physical coupling of magnetophoresis with (i.e., elasto-inertial) microparticles. Particle lateral migration was studied in coflowing configuration viscoelastic ferrofluid/water (sample/sheath). The particles were suspended ferrofluid confined near channel sidewall by sheath flow. coordination elasto-inertial promoted cross-stream particles. Besides, investigated effect flow rate ratio total Furthermore, also effects fluid elasticity sample flows using different combinations flows, including Newtonian ferrofluid/water, ferrofluid/viscoelastic fluid, coflows. Experimental results demonstrated ascertained PEO-based coflow. Finally, demonstrate proof-of-concept application solution exchange. We envisage that novel multiphysical scheme has great potential flexible versatile microparticles cells.

Язык: Английский

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Three-dimensional inertial focusing based impedance cytometer enabling high-accuracy characterization of electrical properties of tumor cells

Lab on a Chip, Год журнала: 2024, Номер 24(18), С. 4333 - 4343

Опубликована: Янв. 1, 2024

The differences in the cross-sectional positions of cells detection area have a severe negative impact on achieving accurate characterization impedance spectra cells. Herein, we proposed three-dimensional (3D) inertial focusing based cytometer integrating sheath fluid compression and for high-accuracy electrical identification tumor First, studied effects particle initial position focusing. Then, relationship height signal-to-noise ratio (SNR) signal was explored. results showed that efficient single-line 7-20 μm particles close to electrodes achieved signals with high SNR low coefficient variation (CV) were obtained. Finally, properties three types (A549, MDA-MB-231, UM-UC-3 cells) accurately characterized. Machine learning algorithms implemented identify amplitude phase opacities at multiple frequencies. Compared traditional two-dimensional (2D) focusing, accuracy A549, using our 3D increased by 57.5%, 36.4% 36.6%, respectively. enables wide size range without causing clogging obtains signals, improving applicability different complex biological samples cell accuracy.

Язык: Английский

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Effective Boundary Correction for Deterministic Lateral Displacement Microchannels to Improve Cell Separation: A Numerical and Experimental Study

Biosensors, Год журнала: 2024, Номер 14(10), С. 466 - 466

Опубликована: Сен. 29, 2024

Particle separation and sorting techniques based on microfluidics have found extensive applications are increasingly gaining prominence. This research presents the design fabrication of a microfluidic device for separating cells using deterministic lateral displacement (DLD), enabling accuracy continuity while being size-based. Nevertheless, it remains demanding, to completely reverse detrimental effects boundaries that disturb fluidic flow in channel reduce particle efficiency. study introduces novel approach enhance boundary structure channels. By this design, efficiency is boosted, fluid behavior around walls improved. The correction (BC) enhances operation microchannel very effective microchannels. With correction, exhibited improved efficiencies, but its absence, efficiencies dropped. collected microscopic images isolation prostate cancer cell lines red blood revealed promising outcomes. circulating tumor (CTC) throughput channel, quantified as ratio or proportion exiting entering it, exceeds 93%. Moreover, CTC isolation, expressed from upper outlet all cells, over 89%. Additionally, evaluated lower surpasses 77%. While same DLD separator without reduced by 5%.

Язык: Английский

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High‐Viability Circulating Tumor Cells Sorting From Whole Blood at Single Cell Level Using Laser‐Induced Forward Transfer‐Assisted Microfiltration

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Янв. 27, 2025

The efficient isolation and molecular analysis of circulating tumor cells (CTCs) from whole blood at single-cell level are crucial for understanding metastasis developing personalized treatments. viability isolated is the key prerequisite downstream analysis, especially RNA sequencing. This study develops a laser-induced forward transfer -assisted microfiltration system (LIFT-AMFS) high-viability CTC enrichment retrieval blood. LIFT-compatible double-stepped microfilter (DSMF), central to this system, comprises two micropore layers: lower layer's smaller micropores facilitate size-based cell separation, upper larger enable liquid encapsulating captured cells. By optimizing design DSMFs, has capture efficiency 88% processing throughput up 15.0 mL min-1 during microfilter-based size screening stage, with yield over 95% stage. retrieved single cells, high viability, qualified ex vivo culture direct cDNA CTCs surpassed 4.5 ng, sufficient library construction. All sequencing data exhibited Q30 scores above 95.92%. LIFT-AMFS shows promise in cellular biomedical research.

Язык: Английский

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Pleural effusion as a sample matrix for laboratory analyses in cancer management: a perspective

Clinical Chemistry and Laboratory Medicine (CCLM), Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Abstract Malignant effusions, pleural effusion or ascites, represent a common problem in cancer patients. Pleural patient may be caused also by non-neoplastic conditions, and the diagnosis of malignant is established demonstration tumor cells fluid. Microscopical detection fluid often fails, there an unmet medical need for more sensitive methods. New approaches, including isolation using magnetic beads coated with monoclonal antibodies targeting antigens expressed on not only increase diagnostic sensitivity, but provide material analysis predictive biomarkers. The advent new technologies illustrates incremental role laboratory medicine management patients effusions.

Язык: Английский

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Direct Isolation of Multiple Types of Circulating Tumor Cells from Undiluted Human Blood Using Cascaded Viscoelastic Microfluidics

Biosensors and Bioelectronics, Год журнала: 2025, Номер 278, С. 117359 - 117359

Опубликована: Март 11, 2025

Язык: Английский

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Efficient Extraction of White Blood Cells Using Modular Inertial Microfluidics

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

The existence of massive amounts red blood cells (RBCs) and other cell components poses a challenge for the efficient extraction white (WBCs) from blood. Herein, we proposed modular inertial microfluidics WBCs lysed samples. different WBC needs could be met by flexibly combining washing module concentration in microfluidics. First, respective optimal flow rates modules with channel sizes were explored. Then, based on combination modules, three modes to meet processing large-volume sample, low volume expansion, easy operation. replacement efficiency recovery rate target particles all greater than 93%. Finally, samples was tested. combined use Modes 1 2 achieved 93.1% 93.6%, while single Mode 3 97.1% 95.9%. Compared traditional centrifuges, our showed better performance reduced residual RBC fragments. More importantly, it provided an excellent free capability needs.

Язык: Английский

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