Isothermal nucleic acid amplification-based biosensors: The next generation analytical toolkit for point-of-care assay of foodborne pathogens

Trends in Food Science & Technology, Год журнала: 2025, Номер unknown, С. 104882 - 104882

Опубликована: Янв. 1, 2025

Язык: Английский

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Sample-in-answer-out centrifugal microfluidic chip reaction biosensor powered by Thermus thermophilus Argonaute (TtAgo) for rapid, highly sensitive and multiplexed molecular diagnostics of foodborne bacterial pathogens

Chemical Engineering Journal, Год журнала: 2024, Номер 495, С. 153434 - 153434

Опубликована: Июль 1, 2024

Язык: Английский

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Argonaute-Based Nucleic Acid Detection Technology: Advantages, Current Status, Challenges, and Perspectives

ACS Sensors, Год журнала: 2024, Номер 9(11), С. 5665 - 5682

Опубликована: Ноя. 11, 2024

Rapid and accurate detection is a prerequisite for precise clinical diagnostics, ensuring food safety, facilitating biotechnological applications. The Argonaute system, as cutting-edge technique, has been successfully repurposed in biosensing beyond the CRISPR/Cas system (clustered regularly interspaced short palindromic repeats CRISPR-associated proteins), which extensively researched, but recognition of PAM sequences remains restricted. Argonaute, programmable target-activated nuclease, fabricating novel methods due to its unparalleled biological features. In this comprehensive review, we initially elaborate on current nucleic acid testing nucleases, followed by delving into structure nuclease activity system. advantages compared with are highlighted discussed. Furthermore, summarize applications Argonaute-based provide an in-depth analysis future perspectives challenges. Recent research demonstrated that innovative rapidly advancing technology can overcome limitations existing potentially replace them. summary, implementation integration other technologies hold promise developing customized intelligent across various aspects.

Язык: Английский

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Programmable and ultra-efficient Argonaute protein-mediated nucleic acid tests: A review

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 278, С. 134755 - 134755

Опубликована: Авг. 13, 2024

Язык: Английский

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Versatile sensing strategies based on emerging programmable prokaryotic Argonautes: From nucleic acid to non-nucleic acid targets

TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118130 - 118130

Опубликована: Янв. 1, 2025

Язык: Английский

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CuO2@SiO2 nanoparticle assisted click reaction-mediated magnetic relaxation biosensor for rapid detection of Salmonella in food

Biosensors and Bioelectronics, Год журнала: 2025, Номер 273, С. 117188 - 117188

Опубликована: Янв. 21, 2025

Язык: Английский

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Argonaute protein powered biosensing for pathogenic biosafety

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 305, С. 141321 - 141321

Опубликована: Фев. 19, 2025

Язык: Английский

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(Co, S)-Codoped BiOBr as a novel signal probe coupled with LAMP (H+)-pH responsive photoelectrochemistry biosensor for ultrasensitive detection of microRNA

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 422, С. 136482 - 136482

Опубликована: Авг. 22, 2024

Язык: Английский

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CRISPR/Cas and Argonaute-powered lateral flow assay for pathogens detection

Critical Reviews in Food Science and Nutrition, Год журнала: 2024, Номер unknown, С. 1 - 23

Опубликована: Окт. 21, 2024

Pathogens contamination is a pressing global public issue that has garnered significant attention worldwide, especially in light of recent outbreaks foodborne illnesses. Programmable nucleases like CRISPR/Cas and Argonaute hold promise as tools for nucleic acid testing owning to programmability the precise target sequence specificity, which been utilized development pathogens detection. At present, fluorescence, main signal output method, provides simple response mode sensing analysis. However, dependence fluorescence on large instruments correct analysis data limited its use remote areas. Lateral flow strips (LFS), emerging novel flexible substrate, offer plethora advantages, encompassing easy-to-use, rapidity, visualization, low-cost, portability, etc. The integration with LFS, lateral assay (LFA), rendered new on-site In review, we introduced two programmable Argonaute, followed by structure, principle advantages LFA. Then diversified engineering detection pattens viruses, bacteria, parasites, fungi based were summarized. Finally, challenge perspectives involved diagnostic assays discussed.

Язык: Английский

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Trends in the use of Argonaute Proteins in Molecular Diagnosis

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер unknown, С. 118081 - 118081

Опубликована: Ноя. 1, 2024

Язык: Английский

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