Nanoscale Extracellular Vesicle-Enabled Liquid Biopsy: Advances and Challenges for Lung Cancer Detection DOI Creative Commons
Adeel Khan, Faisal Raza, Nongyue He

и другие.

Micromachines, Год журнала: 2024, Номер 15(10), С. 1181 - 1181

Опубликована: Сен. 24, 2024

Lung cancer is responsible for the death of over a million people worldwide every year. With its high mortality rate and exponentially growing number new cases, lung major threat to public health. The poor survival rates patients can be attributed stealth progression late diagnosis. For long time, intrusive tissue biopsy has been considered gold standard diagnosis subtyping; however, intrinsic limitations cannot overlooked. In addition being invasive costly, it also suffers from in sensitivity specificity, not suitable repeated sampling, provides restricted information about tumor molecular landscape, inaccessible several cases. To cope with this, advancements diagnostic technologies, such as liquid biopsy, have shown great prospects. Liquid an innovative non-invasive approach which cancer-related components called biomarkers are detected body fluids, blood, urine, saliva others. It offers less alternative potential applications routine screening, predicting treatment outcomes, evaluating effectiveness, detecting residual disease, or disease recurrence. A large research articles indicated extracellular vesicles (EVs) ideal biopsy. EVs heterogeneous collection membranous nanoparticles diverse sizes, contents, surface markers. play critical role pathophysiological states gained prominence prognostic multiple diseases, including cancer. this review, we provide detailed overview EV-based Moreover, highlights strengths weaknesses various contemporary techniques EV isolation analysis challenges that need addressed ensure widespread clinical application biopsies summary, present interesting opportunities development novel platforms cancer, one most abundant cancers millions deaths worldwide.

Язык: Английский

Colorimetric aptasensor coupled with a deep-learning-powered smartphone app for programmed death ligand-1 expressing extracellular vesicles DOI Creative Commons
Adeel Khan, Haroon Latif Khan, Nongyue He

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 15

Опубликована: Янв. 23, 2025

Lung cancer is a devastating public health threat and leading cause of cancer-related deaths. Therefore, it imperative to develop sophisticated techniques for the non-invasive detection lung cancer. Extracellular vesicles expressing programmed death ligand-1 (PD-L1) markers (PD-L1@EVs) in blood are reported be indicative response immunotherapy. Our approach development colorimetric aptasensor by combining rapid capturing efficiency (Fe 3 O 4 )-SiO 2 -TiO EV isolation with PD-L1 aptamer-triggered enzyme-linked hybridization chain reaction (HCR) signal amplification. The numerous HRPs catalyze their substrate dopamine (colorless) into polydopamine (blackish brown). Change chromaticity directly correlates concentration PD-L1@EVs sample. was able detect at concentrations as low 3.6×10 EVs/mL wide linear range from 10 high specificity successfully detected patients’ serum healthy volunteers’ serum. To transform qualitative quantitative operation, we developed an intelligent convolutional neural network (CNN)-powered analyzer form smartphone app named ExoP, thereby achieving analysis minimal user intervention or additional hardware attachments sensitive specific quantification PD-L1@EVs. This combined offers simple, sensitive, tool using addition CNN-powered further eliminates need specialized equipment, making more accessible low-resource settings.

Язык: Английский

Процитировано

0
Recent advances of surface plasmon resonance sensors based on metal-organic frameworks DOI
Chuanpan Guo, Zhenzhen Liu, Yihan He

и другие.

Talanta, Год журнала: 2025, Номер unknown, С. 128008 - 128008

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Emerging Sensing Technologies for Liquid Biopsy Applications: Steps Closer to Personalized Medicine DOI Creative Commons
Panagiota M. Kalligosfyri, Eleni Lamprou, Despina P. Kalogianni

и другие.

Sensors, Год журнала: 2024, Номер 24(24), С. 7902 - 7902

Опубликована: Дек. 11, 2024

Liquid biopsy is an efficient diagnostic/prognostic tool for tumor-derived component detection in peripheral circulation and other body fluids. The rapid assessment of liquid techniques facilitates early cancer diagnosis prognosis. Early precise tumor biomarkers provides crucial information about the that guides clinicians towards effective personalized medicine. Point-of-care-testing remains still a great challenge diagnostics. promising alternative to tissue with advantages less invasion real-time monitoring disease, also providing heterogeneity. field continuously rapidly expanding. Numerous sophisticated biosensors have been developed targeting several achieve low limits, increased specificity robustness. Current include mainly optical sensors, such as colorimetric, fluorescent, SPR, SERS lateral flow assays. Electrochemical sensors developed, very limits. Colorimetric exhibited simplicity signal interpretation, while fluorescent contributed analysis times, SPR/SERS enabled label-free analysis. Novel target amplification enhancement exploited increase detectability sensors. In this context, review focused on recent advances biosensing technology cutting-edge applications point-of-care testing.

Язык: Английский

Процитировано

2
Nanoscale Extracellular Vesicle-Enabled Liquid Biopsy: Advances and Challenges for Lung Cancer Detection DOI Creative Commons
Adeel Khan, Faisal Raza, Nongyue He

и другие.

Micromachines, Год журнала: 2024, Номер 15(10), С. 1181 - 1181

Опубликована: Сен. 24, 2024

Lung cancer is responsible for the death of over a million people worldwide every year. With its high mortality rate and exponentially growing number new cases, lung major threat to public health. The poor survival rates patients can be attributed stealth progression late diagnosis. For long time, intrusive tissue biopsy has been considered gold standard diagnosis subtyping; however, intrinsic limitations cannot overlooked. In addition being invasive costly, it also suffers from in sensitivity specificity, not suitable repeated sampling, provides restricted information about tumor molecular landscape, inaccessible several cases. To cope with this, advancements diagnostic technologies, such as liquid biopsy, have shown great prospects. Liquid an innovative non-invasive approach which cancer-related components called biomarkers are detected body fluids, blood, urine, saliva others. It offers less alternative potential applications routine screening, predicting treatment outcomes, evaluating effectiveness, detecting residual disease, or disease recurrence. A large research articles indicated extracellular vesicles (EVs) ideal biopsy. EVs heterogeneous collection membranous nanoparticles diverse sizes, contents, surface markers. play critical role pathophysiological states gained prominence prognostic multiple diseases, including cancer. this review, we provide detailed overview EV-based Moreover, highlights strengths weaknesses various contemporary techniques EV isolation analysis challenges that need addressed ensure widespread clinical application biopsies summary, present interesting opportunities development novel platforms cancer, one most abundant cancers millions deaths worldwide.

Язык: Английский

Процитировано

2