Evolving CRISPR/Cas system for food safety monitoring across the food supply chain DOI
Jianjun Shen, Di Zhang, Yanyan He

и другие.

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 181, С. 118050 - 118050

Опубликована: Ноя. 14, 2024

Язык: Английский

Recent advances in centrifugal microfluidics for point-of-care testing DOI
Huijuan Yuan,

Zeyu Miao,

Chao Wan

и другие.

Lab on a Chip, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Centrifugal microfluidics, with its advantages of rapid and precise fluid control without the need for external pressure, is widely applied in point-of-care testing.

Язык: Английский

Процитировано

3
Lift-CM: An integrated Lift-heater Centrifugal Microfluidic Platform for point-of-care pathogen nucleic acid detection using Isothermal amplification and CRISPR/Cas12a DOI

Taowei Shu,

Xueer Yin,

Qihua Xiong

и другие.

Biosensors and Bioelectronics, Год журнала: 2025, Номер 274, С. 117178 - 117178

Опубликована: Янв. 18, 2025

Язык: Английский

Процитировано

2
CRISPR/dCas9-assisted SERS sensing for transgenic crops based on two-factor verification of 35S promoter and Nos terminator DOI

Ailing Su,

Ziqi Chen, Weiqing Xu

и другие.

Sensors and Actuators B Chemical, Год журнала: 2025, Номер 429, С. 137293 - 137293

Опубликована: Янв. 27, 2025

Язык: Английский

Процитировано

1
A rolling circle mediated exponential amplification reaction with suppressed nonspecific amplification to detect pathogen RNA with high sensitivity DOI
Yao Liu, Yang Li, Yuting Shan

и другие.

Analytical and Bioanalytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 22, 2025

Язык: Английский

Процитировано

0
CRISPR/Cas‐SERS Sensing Platforms: A Frontier Technology for Next‐Generation Fast, Low‐Cost, Ultra‐Micro Biosample Detection DOI
Chengxin Bao, Xiangguo Liu, Chongyang Liang

и другие.

Journal of Raman Spectroscopy, Год журнала: 2025, Номер unknown

Опубликована: Апрель 24, 2025

ABSTRACT Researchers have long been interested in nucleic acid detection technology. Surface‐enhanced Raman spectroscopy (SERS) is distinguished by its high sensitivity, minimal sample volume, resistance to fluorescence interference, cost‐effectiveness, and rapidity compared conventional analysis methods. The clustered regularly interspaced short palindromic repeats/CRISPR‐associated protein (CRISPR/Cas), a novel gene editing tool, has garnered significant interest due precise identification isothermal advantages. Integrating the CRISPR/Cas system's specific capabilities with high‐sensitivity fingerprinting properties of SERS offers sensitive, ultra‐low rapid, straightforward method for detecting new modalities. This review delineates components characteristics system, encompassing three Cas proteins (Cas9, Cas12, Cas13) technologies derived from CRISPR/Cas, namely, enzymatic reporter unlocking (SHERLOCK) DNA endonuclease‐targeted CRISPR trans reporters (DETECTR). Advancements CRISPR/Cas‐SERS‐based assays were emphasized, traditional non‐nucleic tests. Examples encompass microfluidics/microdroplet‐based CRISPR/Cas‐SERS non‐amplified based on CRISPR/Cas‐SERS, so on.

Язык: Английский

Процитировано

0
Evolving CRISPR/Cas system for food safety monitoring across the food supply chain DOI
Jianjun Shen, Di Zhang, Yanyan He

и другие.

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 181, С. 118050 - 118050

Опубликована: Ноя. 14, 2024

Язык: Английский

Процитировано

1