Programmable DNA Nanoswitch-Regulated Plasmonic CRISPR/Cas12a-Gold Nanostars Reporter Platform for Nucleic Acid and Non-Nucleic Acid Biomarker Analysis Assisted by a Spatial Confinement Effect DOI

Congkai Wang,

Xiaohan Xu, Yao Wang

и другие.

Nano Letters, Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

CRISPR/Cas 12a system based nucleic acid and non-nucleic targets detection faces two challenges including (1) multiple crRNAs are needed for biomarkers (2) insufficient sensitivity resulted from photobleaching of fluorescent dyes the low kinetic cleavage rate a traditional single-strand (ssDNA) reporter. To address these limitations, we developed programmable DNA nanoswitch (NS)-regulated plasmonic CRISPR/Cas12a-gold nanostars (Au NSTs) reporter platform with assistance spatial confinement effect. Through simply programming target recognition sequence in NS, only one crRNA is required to detect both biomarkers. The limit decreased by ∼196-fold miRNA-375 122-fold prostate-specific antigen (PSA), respectively. Moreover, versatile evaluation PSA clinical urine samples can also be achieved, according which prostate cancer healthy groups well identified.

Язык: Английский

Programmable DNA Nanoswitch-Regulated Plasmonic CRISPR/Cas12a-Gold Nanostars Reporter Platform for Nucleic Acid and Non-Nucleic Acid Biomarker Analysis Assisted by a Spatial Confinement Effect DOI

Congkai Wang,

Xiaohan Xu, Yao Wang

и другие.

Nano Letters, Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

CRISPR/Cas 12a system based nucleic acid and non-nucleic targets detection faces two challenges including (1) multiple crRNAs are needed for biomarkers (2) insufficient sensitivity resulted from photobleaching of fluorescent dyes the low kinetic cleavage rate a traditional single-strand (ssDNA) reporter. To address these limitations, we developed programmable DNA nanoswitch (NS)-regulated plasmonic CRISPR/Cas12a-gold nanostars (Au NSTs) reporter platform with assistance spatial confinement effect. Through simply programming target recognition sequence in NS, only one crRNA is required to detect both biomarkers. The limit decreased by ∼196-fold miRNA-375 122-fold prostate-specific antigen (PSA), respectively. Moreover, versatile evaluation PSA clinical urine samples can also be achieved, according which prostate cancer healthy groups well identified.

Язык: Английский

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