Neural crest lineage analysis: from past to future trajectory DOI Creative Commons
Weiyi Tang, Marianne Bronner‐Fraser

Development, Год журнала: 2020, Номер 147(20)

Опубликована: Окт. 15, 2020

ABSTRACT Since its discovery 150 years ago, the neural crest has intrigued investigators owing to remarkable developmental potential and extensive migratory ability. Cell lineage analysis been an essential tool for exploring cell fate migration routes. By marking progenitor cells, one can observe their subsequent locations types into which they differentiate. Here, we review major discoveries in tracing from a historical perspective. We discuss how advancing technologies have refined lineage-tracing studies, clonal be applied questions regarding multipotency. also highlight effective tracing, when combined with recently developed molecular imaging tools, such as single-cell transcriptomics, single-molecule fluorescence situ hybridization high-resolution imaging, extend scope of studies beyond development regeneration cancer initiation.

Язык: Английский

Exercise promotes satellite cell contribution to myofibers in a load-dependent manner DOI Creative Commons

Evi Masschelein,

Gommaar D’Hulst, Joel Zvick

и другие.

Skeletal Muscle, Год журнала: 2020, Номер 10(1)

Опубликована: Июль 9, 2020

Abstract Background Satellite cells (SCs) are required for muscle repair following injury and involved in remodeling upon muscular contractions. Exercise stimulates SC accumulation myonuclear accretion. To what extent exercise training at different mechanical loads drive contribution to myonuclei however is unknown. Results By performing fate tracing experiments, we show that 8 weeks of voluntary wheel running increased myofibers mouse plantar flexor muscles a load-dependent, but fiber type-independent manner. Increased fusion was not exclusively linked hypertrophy as without external load substantially the absence hypertrophy. Due nuclear propagation, fluorescent models were inadequate quantify myonuclei. Ultimately, by DNA level, mirrors accretion during exercise. Conclusions Collectively, independently promotes existing myofibers. Also, due propagation reporter proteins, our data warrant caution use quantitative evaluation satellite cell

Язык: Английский

Процитировано

70
The Stat3-Fam3a axis promotes muscle stem cell myogenic lineage progression by inducing mitochondrial respiration DOI Creative Commons

David Sala,

Thomas J. Cunningham, Michael J. Stec

и другие.

Nature Communications, Год журнала: 2019, Номер 10(1)

Опубликована: Апрель 17, 2019

Abstract Metabolic reprogramming is an active regulator of stem cell fate choices, and successful differentiation in different compartments requires the induction oxidative phosphorylation. However, mechanisms that promote mitochondrial respiration during are poorly understood. Here we demonstrate Stat3 promotes muscle myogenic lineage progression by stimulating mice. We identify Fam3a, a cytokine-like protein, as major downstream effector cells. Fam3a required for commitment skeletal development. show cells secrete exposure Stat3-ablated to recombinant vitro vivo rescues their defects commitment. Together, these findings indicate Stat3-regulated secreted factor metabolism differentiation, suggests potential tool modulate choices.

Язык: Английский

Процитировано

59
Muscle Stem Cell Quiescence: Controlling Stemness by Staying Asleep DOI
Sara Ancel, Pascal Stuelsatz, Jérôme N. Feige

и другие.

Trends in Cell Biology, Год журнала: 2021, Номер 31(7), С. 556 - 568

Опубликована: Март 2, 2021

Язык: Английский

Процитировано

56
Acetylation of PAX7 controls muscle stem cell self-renewal and differentiation potential in mice DOI Creative Commons
Marie‐Claude Sincennes, Caroline Brun, Alexander Y. Lin

и другие.

Nature Communications, Год журнала: 2021, Номер 12(1)

Опубликована: Май 31, 2021

Abstract Muscle stem cell function has been suggested to be regulated by Acetyl-CoA and NAD+ availability, but the mechanisms remain unclear. Here we report identification of two acetylation sites on PAX7 that positively regulate its transcriptional activity. Lack reduces DNA binding, specifically homeobox motif. The acetyltransferase MYST1 stimulated Acetyl-CoA, deacetylase SIRT2 NAD +, are identified as direct regulators asymmetric division in muscle cells. Abolishing mice using CRISPR/Cas9 mutagenesis leads an expansion satellite pool, reduced numbers divisions, increased oxidative IIA myofibers. Gene expression analysis confirms lack preferentially affects target genes homeodomain binding motifs. Therefore, status regulates differentiation potential facilitate metabolic adaptation tissue.

Язык: Английский

Процитировано

56
Neural crest lineage analysis: from past to future trajectory DOI Creative Commons
Weiyi Tang, Marianne Bronner‐Fraser

Development, Год журнала: 2020, Номер 147(20)

Опубликована: Окт. 15, 2020

ABSTRACT Since its discovery 150 years ago, the neural crest has intrigued investigators owing to remarkable developmental potential and extensive migratory ability. Cell lineage analysis been an essential tool for exploring cell fate migration routes. By marking progenitor cells, one can observe their subsequent locations types into which they differentiate. Here, we review major discoveries in tracing from a historical perspective. We discuss how advancing technologies have refined lineage-tracing studies, clonal be applied questions regarding multipotency. also highlight effective tracing, when combined with recently developed molecular imaging tools, such as single-cell transcriptomics, single-molecule fluorescence situ hybridization high-resolution imaging, extend scope of studies beyond development regeneration cancer initiation.

Язык: Английский

Процитировано

51