Exercise promotes satellite cell contribution to myofibers in a load-dependent manner

Skeletal Muscle, Journal Year: 2020, Volume and Issue: 10(1)

Published: July 9, 2020

Abstract Background Satellite cells (SCs) are required for muscle repair following injury and involved in remodeling upon muscular contractions. Exercise stimulates SC accumulation myonuclear accretion. To what extent exercise training at different mechanical loads drive contribution to myonuclei however is unknown. Results By performing fate tracing experiments, we show that 8 weeks of voluntary wheel running increased myofibers mouse plantar flexor muscles a load-dependent, but fiber type-independent manner. Increased fusion was not exclusively linked hypertrophy as without external load substantially the absence hypertrophy. Due nuclear propagation, fluorescent models were inadequate quantify myonuclei. Ultimately, by DNA level, mirrors accretion during exercise. Conclusions Collectively, independently promotes existing myofibers. Also, due propagation reporter proteins, our data warrant caution use quantitative evaluation satellite cell

Language: Английский

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The Stat3-Fam3a axis promotes muscle stem cell myogenic lineage progression by inducing mitochondrial respiration

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: April 17, 2019

Abstract Metabolic reprogramming is an active regulator of stem cell fate choices, and successful differentiation in different compartments requires the induction oxidative phosphorylation. However, mechanisms that promote mitochondrial respiration during are poorly understood. Here we demonstrate Stat3 promotes muscle myogenic lineage progression by stimulating mice. We identify Fam3a, a cytokine-like protein, as major downstream effector cells. Fam3a required for commitment skeletal development. show cells secrete exposure Stat3-ablated to recombinant vitro vivo rescues their defects commitment. Together, these findings indicate Stat3-regulated secreted factor metabolism differentiation, suggests potential tool modulate choices.

Language: Английский

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Muscle Stem Cell Quiescence: Controlling Stemness by Staying Asleep

Trends in Cell Biology, Journal Year: 2021, Volume and Issue: 31(7), P. 556 - 568

Published: March 2, 2021

Language: Английский

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Acetylation of PAX7 controls muscle stem cell self-renewal and differentiation potential in mice

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: May 31, 2021

Abstract Muscle stem cell function has been suggested to be regulated by Acetyl-CoA and NAD+ availability, but the mechanisms remain unclear. Here we report identification of two acetylation sites on PAX7 that positively regulate its transcriptional activity. Lack reduces DNA binding, specifically homeobox motif. The acetyltransferase MYST1 stimulated Acetyl-CoA, deacetylase SIRT2 NAD +, are identified as direct regulators asymmetric division in muscle cells. Abolishing mice using CRISPR/Cas9 mutagenesis leads an expansion satellite pool, reduced numbers divisions, increased oxidative IIA myofibers. Gene expression analysis confirms lack preferentially affects target genes homeodomain binding motifs. Therefore, status regulates differentiation potential facilitate metabolic adaptation tissue.

Language: Английский

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Transcriptional networks controlling stromal cell differentiation

Nature Reviews Molecular Cell Biology, Journal Year: 2021, Volume and Issue: 22(7), P. 465 - 482

Published: April 9, 2021

Language: Английский

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