Local immune recognition of trophoblast in early human pregnancy: controversies and questions

Nature reviews. Immunology, Год журнала: 2022, Номер 23(4), С. 222 - 235

Опубликована: Окт. 3, 2022

Язык: Английский

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Modeling post-implantation stages of human development into early organogenesis with stem-cell-derived peri-gastruloids

Cell, Год журнала: 2023, Номер 186(18), С. 3776 - 3792.e16

Опубликована: Июль 20, 2023

Язык: Английский

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Hallmarks of totipotent and pluripotent stem cell states

Cell stem cell, Год журнала: 2024, Номер 31(3), С. 312 - 333

Опубликована: Фев. 20, 2024

Though totipotency and pluripotency are transient during early embryogenesis, they establish the foundation for development of all mammals. Studying these in vivo has been challenging due to limited access ethical constraints, particularly humans. Recent progress led diverse culture adaptations epiblast cells vitro form totipotent pluripotent stem cells, which not only deepen our understanding embryonic but also serve as invaluable resources animal reproduction regenerative medicine. This review delves into hallmarks shedding light on their key molecular functional features.

Язык: Английский

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Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells

Cell stem cell, Год журнала: 2022, Номер 29(9), С. 1346 - 1365.e10

Опубликована: Сен. 1, 2022

A hallmark of primate postimplantation embryogenesis is the specification extraembryonic mesoderm (EXM) before gastrulation, in contrast to rodents where this tissue formed only after gastrulation. Here, we discover that naive human pluripotent stem cells (hPSCs) are competent differentiate into EXM (EXMCs). EXMCs specified by inhibition Nodal signaling and GSK3B, maintained mTOR BMP4 activity, their transcriptome epigenome closely resemble monkey embryo EXM. mesenchymal, can arise from an epiblast intermediate, capable self-renewal. Thus, arising via primate-specific between implantation gastrulation be modeled vitro. We also find most rare off-target within blastoids triple (Kagawa et al., 2021) correspond EXMCs. Our study impacts our ability model molecular mechanisms early related defects.

Язык: Английский

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Complex regulatory networks influence pluripotent cell state transitions in human iPSCs

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Фев. 23, 2024

Stem cells exist in vitro a spectrum of interconvertible pluripotent states. Analyzing hundreds hiPSCs derived from different individuals, we show the proportions these states vary considerably across lines. We discover 13 gene network modules (GNMs) and regulatory (RNMs), which are highly correlated with each other suggesting that coordinated co-accessibility elements RNMs likely underlie expression genes GNMs. Epigenetic analyses reveal networks underlying self-renewal pluripotency more complex than previously realized. Genetic identify thousands variants overlapped predicted transcription factor binding sites associated chromatin accessibility hiPSCs. master regulator pluripotency, NANOG-OCT4 Complex, its significantly enriched for large effects, they play role varying cellular between Our work bins tens into discrete networks, shows processes have surprising level complexity, suggests genetic factors may contribute to cell state transitions human iPSC

Язык: Английский

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Why study human embryo development?

Developmental Biology, Год журнала: 2024, Номер 509, С. 43 - 50

Опубликована: Фев. 5, 2024

Understanding the processes and mechanisms underlying early human embryo development has become an increasingly active important area of research. It potential for insights into clinical issues such as pregnancy loss, origins congenital anomalies developmental adult disease, well fundamental biology. Improved culture systems preimplantation embryos, combined with new tools single cell genomics live imaging, are providing similarities differences between mouse development. However, access to material is still restricted extended embryos regulatory ethical concerns. Stem cell-derived models different phases can potentially overcome these limitations provide a scalable source explore postimplantation stages To date, clearly incomplete replicas normal but future technological improvements be envisaged. The environment studies remains fully resolved.

Язык: Английский

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Lumen expansion is initially driven by apical actin polymerization followed by osmotic pressure in a human epiblast model

Cell stem cell, Год журнала: 2024, Номер 31(5), С. 640 - 656.e8

Опубликована: Май 1, 2024

Язык: Английский

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Dissecting embryonic and extraembryonic lineage crosstalk with stem cell co-culture

Cell, Год журнала: 2023, Номер 186(26), С. 5859 - 5875.e24

Опубликована: Дек. 1, 2023

Язык: Английский

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Esrrb guides naive pluripotent cells through the formative transcriptional programme

Nature Cell Biology, Год журнала: 2023, Номер 25(5), С. 643 - 657

Опубликована: Апрель 27, 2023

Язык: Английский

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Sensing and guiding cell-state transitions by using genetically encoded endoribonuclease-mediated microRNA sensors

Nature Biomedical Engineering, Год журнала: 2024, Номер unknown

Опубликована: Июль 9, 2024

Язык: Английский

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