Cells,
Год журнала:
2023,
Номер
12(8), С. 1192 - 1192
Опубликована: Апрель 20, 2023
Pluripotent
stem
cells
(PSCs;
embryonic
and
induced
pluripotent
cells)
can
recapitulate
critical
aspects
of
the
early
stages
development;
therefore,
they
became
a
powerful
tool
for
in
vitro
study
molecular
mechanisms
that
underlie
blastocyst
formation,
implantation,
spectrum
pluripotency
beginning
gastrulation,
among
other
processes.
Traditionally,
PSCs
were
studied
2D
cultures
or
monolayers,
without
considering
spatial
organization
developing
embryo.
However,
recent
research
demonstrated
form
3D
structures
simulate
gastrula
events,
such
as
amniotic
cavity
formation
somitogenesis.
This
breakthrough
provides
an
unparalleled
opportunity
to
human
embryogenesis
by
examining
interactions,
cytoarchitecture
multiple
cell
lineages,
which
have
long
remained
mystery
due
limitations
studying
utero
embryos.
In
this
review,
we
will
provide
overview
how
experimental
embryology
currently
utilizes
models
blastoids,
gastruloids
aggregates
derived
from
advance
our
understanding
intricate
processes
involved
embryo
development.
Stem
cell-based
embryo
models
offer
unprecedented
experimental
tools
for
studying
early
human
development.
The
usefulness
of
hinges
on
their
molecular,
cellular
and
structural
fidelities
to
in
vivo
counterparts.
To
authenticate
models,
single-cell
RNA
sequencing
has
been
utilized
unbiased
transcriptional
profiling.
However,
an
organized
integrated
RNA-sequencing
dataset,
serving
as
a
universal
reference
benchmarking
remains
unavailable.
Here
we
developed
such
through
the
integration
six
published
datasets
covering
development
from
zygote
gastrula.
Lineage
annotations
are
contrasted
validated
with
available
nonhuman
primate
datasets.
Using
stabilized
Uniform
Manifold
Approximation
Projection,
constructed
embryogenesis
prediction
tool,
where
query
can
be
projected
annotated
predicted
cell
identities.
this
examined
highlighting
risk
misannotation
when
relevant
references
not
authentication.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2021,
Номер
unknown
Опубликована: Май 7, 2021
Summary
Stem
cell-based
embryo
models
offer
unprecedented
experimental
tools
for
studying
early
human
development.
The
usefulness
of
hinges
on
their
molecular,
cellular
and
structural
fidelities
to
in
vivo
counterparts.
To
authenticate
models,
single-cell
RNA-sequencing
has
been
utilised
unbiased
transcriptional
profiling.
However,
a
well-organised
integrated
dataset,
serving
as
universal
reference
benchmarking
remains
unavailable.
Herein,
we
developed
such
reference,
through
integration
six
published
datasets
covering
developmental
stages
from
the
zygote
gastrula.
Lineage
annotations
are
contrasted
validated
with
available
non-human
primate
datasets.
Using
stabilised
UMAP
constructed
web
tool,
where
query
can
be
projected
annotated
predicted
cell
identities.
this
examined
several
recent
highlighting
risk
misannotation
when
relevant
references
lacking.
Nature,
Год журнала:
2023,
Номер
626(7998), С. 357 - 366
Опубликована: Дек. 5, 2023
Abstract
Recently,
several
studies
using
cultures
of
human
embryos
together
with
single-cell
RNA-seq
analyses
have
revealed
differences
between
humans
and
mice,
necessitating
the
study
1–8
.
Despite
importance
embryology,
ethical
legal
restrictions
limited
post-implantation-stage
studies.
Thus,
recent
efforts
focused
on
developing
in
vitro
self-organizing
models
stem
cells
9–17
Here,
we
report
genetic
non-genetic
approaches
to
generate
authentic
hypoblast
(naive
hPSC-derived
hypoblast-like
(nHyCs))—known
give
rise
one
two
extraembryonic
tissues
essential
for
embryonic
development—from
naive
pluripotent
(hPSCs).
Our
nHyCs
spontaneously
assemble
hPSCs
form
a
three-dimensional
bilaminar
structure
(bilaminoids)
pro-amniotic-like
cavity.
In
presence
additional
analogues
second
tissue,
trophectoderm,
efficiency
bilaminoid
formation
increases
from
20%
40%,
epiblast
within
bilaminoids
continues
develop
response
trophectoderm-secreted
IL-6.
Furthermore,
show
that
robustly
recapitulate
patterning
anterior–posterior
axis
reflecting
pregastrula
stage,
emergence
which
can
be
shaped
by
genetically
manipulating
DKK1/OTX2
domain.
We
therefore
successfully
modelled
identified
mechanisms
efficiently
guide
stage-specific
growth
progression
as
it
establishes
post-implantation
landmarks
embryogenesis.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Янв. 2, 2024
Abstract
Primordial
germ
cells
(PGCs)
are
the
embryonic
precursors
of
sperm
and
eggs.
They
transmit
genetic
epigenetic
information
across
generations.
Given
prominent
role
germline
defects
in
diseases
such
as
infertility,
detailed
understanding
human
PGC
(hPGC)
development
has
important
implications
reproductive
medicine
studying
evolution.
Yet,
hPGC
specification
remains
an
elusive
process.
Here,
we
report
induction
hPGC-like
(hPGCLCs)
a
bioengineered
pluripotent
stem
cell
(hPSC)
culture
that
mimics
peri-implantation
development.
In
this
culture,
amniotic
ectoderm-like
(AMLCs),
derived
from
hPSCs,
induce
hPGCLC
hPSCs
through
paracrine
signaling
downstream
ISL1
.
Our
data
further
show
functional
roles
NODAL,
WNT,
BMP
induction.
hPGCLCs
successfully
eight
non-obstructive
azoospermia
(NOA)
participant-derived
hPSC
lines
using
biomimetic
platform,
demonstrating
its
promise
for
screening
applications.
From
deciphering
infection
and
disease
mechanisms
to
identifying
novel
biomarkers
personalizing
treatments,
the
characteristics
of
individual
cells
can
provide
significant
insights
into
a
variety
biological
processes
facilitate
decision‐making
in
biomedical
environments.
Conventional
single‐cell
analysis
methods
are
limited
terms
cost,
contamination
risks,
sample
volumes,
times,
throughput,
sensitivity,
selectivity.
Although
microfluidic
approaches
have
been
suggested
as
low‐cost,
information‐rich,
high‐throughput
alternative
conventional
isolation
methods,
limitations
such
necessary
off‐chip
pre‐
post‐processing
well
systems
designed
for
workflows
restricted
their
applications.
In
this
review,
comprehensive
overview
recent
advances
integrated
microfluidics
on‐chip
three
prominent
application
domains
provided:
investigation
somatic
(particularly
cancer
immune
cells),
stem
cells,
microorganisms.
Also,
use
cell
separation
(e.g.,
dielectrophoresis)
unconventional
or
ways,
which
advance
integration
multiple
systems,
is
discussed.
Finally,
critical
discussion
related
current
how
they
could
be
overcome
provided.
Cell,
Год журнала:
2024,
Номер
187(13), С. 3194 - 3219
Опубликована: Июнь 1, 2024
Developing
functional
organs
from
stem
cells
remains
a
challenging
goal
in
regenerative
medicine.
Existing
methodologies,
such
as
tissue
engineering,
bioprinting,
and
organoids,
only
offer
partial
solutions.
This
perspective
focuses
on
two
promising
approaches
emerging
for
engineering
human
cells:
cell-based
embryo
models
interspecies
organogenesis.
Both
exploit
the
premise
of
guiding
to
mimic
natural
development.
We
begin
by
summarizing
what
is
known
about
early
development
blueprint
recapitulating
organogenesis
both
chimeras.
The
latest
advances
fields
are
discussed
before
highlighting
technological
knowledge
gaps
be
addressed
developing
could
achieved
using
approaches.
conclude
discussing
challenges
facing
modeling
outlining
future
prospects
advancing
toward
generation
tissues
basic
research
translational
applications.
ABSTRACT
Pluripotency,
the
capacity
to
generate
all
cells
of
body,
is
a
defining
property
transient
population
epiblast
found
in
pre-,
peri-
and
post-implantation
mammalian
embryos.
As
development
progresses,
undergo
dynamic
transitions
pluripotency
states,
concurrent
with
specification
extra-embryonic
embryonic
lineages.
Recently,
stem
cell-based
models
pre-
human
have
been
developed
using
that
capture
key
properties
at
different
developmental
stages.
Here,
we
review
early
primate
development,
comparing
states
vivo
cultured
pluripotent
representative
these
states.
We
consider
how
status
starting
influences
embryo
and,
turn,
what
can
learn
about
epiblast.
Finally,
discuss
limitations
questions
arising
from
pioneering
studies
this
emerging
field.
