International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(16), С. 8815 - 8815
Опубликована: Авг. 13, 2024
The
adult
mammalian
heart
has
been
demonstrated
to
be
endowed
with
low
but
real
turnover
capacity,
especially
for
cardiomyocytes,
the
key
functional
cell
type.
source,
however,
of
that
capacity
remains
controversial.
In
this
regard,
we
have
defined
and
characterized
a
resident
multipotent
cardiac
mouse
progenitor
population,
Bmi1+DR
(for
Bmi1+
Damage-Responsive
cells).
is
one
types
lowest
ROS
(Reactive
Oxygen
Species)
levels
in
heart,
being
particularly
by
their
close
relationship
vessels,
most
probably
involved
regulation
proliferation/maintenance
Bmi1+DR.
This
was
proposed
work
as
endothelial
niche.
Due
scarcity
cells
generated
an
immortalization/dis-immortalization
model
using
Simian
Vacuolating
Virus
40-Large
Antigen
T
(SV40-T)
facilitate
vitro
characterization.
We
obtained
heterogeneous
population
immortalized
(Bmi1+DRIMM)
validated
attending
different
criteria,
also
showing
comparable
sensitivity
strong
oxidative
damage.
Then,
concluded
Bmi1-DRIMM
appropriate
primary
studies.
co-culture
Bmi1+DRIMM
protects
them
against
damage,
moderate
depletion
non-canonical
autophagy
contributing
modest
metabolic
regulation.
Cell,
Год журнала:
2024,
Номер
187(14), С. 3461 - 3495
Опубликована: Июнь 20, 2024
Developmental
biology-the
study
of
the
processes
by
which
cells,
tissues,
and
organisms
develop
change
over
time-has
entered
a
new
golden
age.
After
molecular
genetics
revolution
in
80s
90s
diversification
field
early
21st
century,
we
have
phase
when
powerful
technologies
provide
approaches
open
unexplored
avenues.
Progress
has
been
accelerated
advances
genomics,
imaging,
engineering,
computational
biology
emerging
model
systems
ranging
from
tardigrades
to
organoids.
We
summarize
how
revolutionary
led
remarkable
progress
understanding
animal
development.
describe
classic
questions
gene
regulation,
pattern
formation,
morphogenesis,
organogenesis,
stem
cell
are
being
revisited.
discuss
connections
development
with
evolution,
self-organization,
metabolism,
time,
ecology.
speculate
developmental
might
evolve
an
era
synthetic
biology,
artificial
intelligence,
human
engineering.
Developmental Biology,
Год журнала:
2024,
Номер
516, С. 71 - 81
Опубликована: Июль 24, 2024
The
dentition
is
critical
to
animal
survival
and
teeth
are
present
in
modern
vertebrates
including
teleost
fish,
sharks,
amphibians,
mammals
reptiles.
developmental
processes
that
give
rise
not
just
preserved
through
evolution
but
also
share
high
level
of
similarity
with
the
embryogenesis
other
ectodermal
organs.
In
this
review
we
go
beyond
embryonic
phase
tooth
development
life-long
replacement.
We
will
address
origins
successional
teeth,
location
putative
tissue-resident
stem
cells,
how
de
novo
formation
continues
throughout
life
shed
a
spatially
temporally
controlled
manner.
evidence
dental
epithelium,
which
earliest
recognizable
structure
reptilian
dentition,
serves
as
niche
for
epithelial
cells
recent
molecular
findings
from
transcriptomics
carried
out
dentitions.
discuss
odontoclasts
resorb
primary
allowing
eruption
tooth.
reptiles,
particularly
lizards,
emerging
some
most
accessible
animals
study
replacement
has
relevance
human
disorders.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 15, 2024
Summary
The
release
and
subsequent
detection
of
nucleic
acids
into
the
cytoplasm
constitute
a
hallmark
radiation-induced
DNA
damage
response.
However,
different
radiation
types,
such
as
photons
protons,
may
elicit
distinct
responses,
uniquely
influencing
normal
stem
cell
activity
tissue
regeneration.
Here,
we
show
that
proton
irradiation
leads
to
enhanced
derepression
transposable
elements
(TEs)
with
consequent
activation
salivary
gland
stem/progenitor
cells.
This
response
is
mediated
by
pronounced
loss
heterochromatin
regulators
accumulation
cytoplasmic
TE-derived
dsRNA,
resulting
in
upregulation
RIG-I
augmented
interferon-beta
(IFN-β)
signaling.
Single
RNA
sequencing
(scRNA-seq)
TE
dynamics
analyses
corroborate
these
findings,
specifically
subpopulation
Sox9
-expressing
cells
increased
INF-β
These
data
reveal
presence
IFN-β
microenvironment
irradiated
organoids
increases
organoid
growth,
pointing
advantages
therapy
over
photon-based
radiotherapy.
Adipose
tissue
regulates
metabolic
balance,
but
aging
disrupts
it,
shifting
fat
from
insulin-sensitive
subcutaneous
to
insulin-resistant
visceral
depots,
impacting
overall
health.
Adipose-derived
stem
cells
(ASCs)
are
crucial
for
regeneration,
diminishes
their
stemness
and
regeneration
potential.
Our
findings
reveal
that
is
associated
with
a
decrease
in
adipose
mass
an
increase
the
depots
mass.
Aging
fibrosis
no
significant
change
adipocyte
size
was
observed
age.
Long
term
caloric
restriction
failed
prevent
fibrotic
changes
resulted
adipocytes
size.
Aged
ASCs
displayed
increased
production
of
ROS.
Using
mitochondrial
membrane
activity
as
indicator
cell
quiescence
senescence,
we
age
exclusively
depot.
In
addition,
aged
accumulated
more
senescent
having
defective
autophagy
activity.
However,
long-term
leads
reduction
ASCs.
Furthermore,
prevents
accumulation
helps
retain
These
results
suggest
mimetics
hold
promise
potential
strategy
rejuvenate
Further
investigations,
including
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(2), С. 671 - 671
Опубликована: Янв. 14, 2025
Human
hematopoietic
stem
cells
(HSCs)
have
traditionally
been
viewed
as
self-renewing,
multipotent
with
enormous
potential
in
sustaining
essential
steady
state
blood
and
immune
cell
production
throughout
life.
Indeed,
around
86%
(1011-1012)
of
new
generated
daily
a
healthy
young
human
adult
are
origin.
Therapeutically,
HSCs
contributed
to
over
1.5
million
transplants
(HCTs)
globally,
making
this
the
most
successful
regenerative
therapy
date.
We
will
commence
review
by
briefly
highlighting
selected
key
achievements
(from
1868
end
20th
century)
that
accomplishment.
Much
our
knowledge
hematopoiesis
is
based
on
small
animal
models
that,
despite
their
importance,
do
not
always
recapitulate
hematopoiesis.
Given
this,
we
critically
progress
challenges
faced
identifying
tracing
lineage
differentiation
trajectories,
referring
murine
studies
needed.
Moving
forward
given
dynamic
can
readily
adjust
variety
stressors,
then
discuss
recent
research
advances
contributing
understanding
(i)
which
HSPCs
maintain
hematopoiesis,
(ii)
where
these
located,
(iii)
mechanisms
come
into
play
when
homeostatic
switches
stress-induced
or
emergency
Abstract
Salivary
gland
stem/progenitor
cells
(SSPCs)
hold
significant
potential
for
regenerative
medicine,
especially
patients
suffering
from
salivary
dysfunction
due
to
various
causes
such
as
radiation
therapy,
Sjögren’s
syndrome,
and
aging.
This
review
provides
a
comprehensive
overview
of
SSPCs,
including
their
characteristics,
isolation,
culture
techniques,
differentiation
pathways,
role
in
tissue
regeneration.
Additionally,
we
highlight
recent
advances
cell-
tissue-based
therapies,
SSPC
transplantation
bioengineered
organ
replacements.
The
challenges
translating
research
into
effective
clinical
therapies
are
also
discussed,
alongside
proposed
solutions
future
directions.
