Sensitive Detection of Biomarker in Gingival Crevicular Fluid Based on Enhanced Electrochemiluminescence by Nanochannel-Confined Co3O4 Nanocatalyst
Biosensors,
Год журнала:
2025,
Номер
15(1), С. 63 - 63
Опубликована: Янв. 19, 2025
The
sensitive
detection
of
inflammatory
biomarkers
in
gingival
crevicular
fluid
(GCF)
is
highly
desirable
for
the
evaluation
periodontal
disease.
Luminol-based
electrochemiluminescence
(ECL)
immunosensors
offer
a
promising
approach
fast
and
convenient
biomarkers.
However,
luminol’s
low
ECL
efficiency
under
neutral
conditions
remains
challenge.
This
study
developed
an
immunosensor
by
engineering
immunorecognition
interface
on
outer
surface
mesoporous
silica
nanochannel
film
(SNF)
confining
Co3O4
nanocatalyst
within
SNF
nanochannels
to
improve
luminol
efficiency.
was
grown
indium
tin
oxide
(ITO)
electrode
using
simple
Stöber
solution
growth
method.
A
successfully
confined
through
situ
electrodeposition,
confirmed
X-ray
photoelectron
spectroscopy
(XPS)
electrochemical
measurements.
demonstrated
excellent
electrocatalytic
activity,
effectively
enhancing
H2O2
oxidation
boosting
signal
conditions.
Using
interleukin-6
(IL-6)
as
proof-of-concept
demonstration,
epoxy
functionalization
enabled
covalent
immobilization
capture
antibodies,
forming
specific
interface.
IL-6
binding
induced
immunocomplex
formation,
which
reduced
allowed
quantitative
detection.
showed
linear
range
from
1
fg
mL−1
10
ng
mL−1,
with
limit
(LOD)
0.64
mL−1.
It
also
good
selectivity
anti-interference
capabilities,
enabling
successful
artificial
GCF
samples.
Язык: Английский
Enhanced electrochemiluminescence of luminol at neutral medium using nanochannel-confined Co3O4 nanozyme for highly sensitive detection of tumor biomarker
Microchemical Journal,
Год журнала:
2025,
Номер
unknown, С. 112903 - 112903
Опубликована: Янв. 1, 2025
Язык: Английский
Homogeneous Aptasensor with Electrochemical and Electrochemiluminescence Dual Detection Channels Enabled by Nanochannel-Based Probe Enrichment and DNase I Cleavage for Tumor Biomarker Detection
Molecules,
Год журнала:
2025,
Номер
30(3), С. 746 - 746
Опубликована: Фев. 6, 2025
Homogeneous
aptasensors
that
eliminate
the
need
for
probe
labeling
or
immobilization
hold
significant
potential
rapid
detection
of
tumor
biomarkers.
Herein,
a
homogeneous
aptasensor
with
electrochemical
(EC)
and
electrochemiluminescence
(ECL)
dual
channels
was
developed
by
integrating
nanochannel-based
enrichment
DNase
I
cleavage
selective
biomarker,
carbohydrate
antigen
125
(CA125).
A
two-dimensional
(2D)
composite
prepared
assembling
CA125-specific
aptamer
cationic
tris(2,2′-bipyridyl)Ru(II)
(Ru(bpy)32+),
which
exhibited
both
EC
ECL
properties,
onto
graphene
oxide
(GO)
nanosheets
(Ru(bpy)32+/Apt@GO).
vertically
ordered
mesoporous
silica
film
(VMSF)
ultrasmall,
uniform,
aligned
nanochannel
arrays
rapidly
grown
on
inexpensive
disposable
indium
tin
(ITO)
electrode,
forming
interface.
Due
to
size
exclusion
effect
ultrasmall
nanochannels
in
VMSF,
Ru(bpy)32+/Apt@GO
unable
penetrate
nanochannels,
resulting
no
detectable
Ru(bpy)32+
signal
electrode.
Upon
specific
recognition
CA125
aptamer,
an
aptamer-CA125
complex
formed
subsequently
detached
from
GO.
then
cleaved
complex,
releasing
allowing
dissociate
into
solution.
This
enzymatic
enabled
re-enter
binding
cycle,
amplifying
release
The
electrostatic
adsorption
VMSF
significantly
enhanced
signals.
constructed
linear
range
0.1
U/mL
100
ng/mL,
limit
(LOD)
91
mU/mL.
For
detection,
detected
over
0.001
U/mL,
LOD
as
low
0.4
demonstrated
excellent
selectivity
successfully
applied
dual-mode
EC/ECL
fetal
bovine
serum
samples.
Язык: Английский
A sensor based on Mo, N, S-doped interconnected porous carbon spheres material for simultaneous determination of hydroquinone and catechol
Microchemical Journal,
Год журнала:
2025,
Номер
unknown, С. 113065 - 113065
Опубликована: Фев. 1, 2025
Язык: Английский
Highly sensitive electrochemical immunosensor based on methylene blue-reduced graphene oxide nanocomposites as signal probes for IL-6 detection in gingival crevicular fluid samples
Frontiers in Chemistry,
Год журнала:
2025,
Номер
13
Опубликована: Апрель 2, 2025
As
an
important
inflammatory
cytokine,
interleukin-6
(IL-6)
can
mediate
the
entire
pathological
process
of
periodontitis
and
is
closely
associated
with
degree
inflammation.
Therefore,
it
critical
to
develop
convenient
quantitative
methods
for
monitoring
IL-6
quantity
in
gingival
crevicular
fluid.
In
this
study,
methylene
blue
(MB)-decorated
reduced
graphene
oxide
(rGO)
employed
as
signal
probe
further
support
antibody-enabling
specific
recognition
IL-6.
Due
π-π
stacking
electrostatic
interactions,
rGO-MB
nanocomposites
be
stably
obtained.
rGO
good
conductivity
large
surface
area
characteristics
promotes
redox
signals
MB
on
glassy
carbon
electrode
(GCE).
addition,
through
simple
situ
self-polymerization
dopamine,
polydopamine
(PDA)
obtained
not
only
directly
used
a
biological
crosslinking
agent
covalent
immobilization
anti-IL-6
antibody
but
also
regarded
protective
layer
enhance
stability
GCE
surface.
Such
designed
PDA/rGO-MB/GCE-based
immunosensor
enables
binding
produces
decreased
electrochemical
MB,
realizing
selective
sensitive
measurement
Consequently,
our
fabricated
has
excellent
linear
relationship
ranging
from
1
pg/mL
100
ng/mL,
limit
detection
low
0.48
pg/mL.
Moreover,
as-prepared
sensing
strategy
shows
accurate
fluid
samples.
Язык: Английский
A dual-functional antibiofouling and signal amplification sensing platform enabling accurate analysis in complicated biological samples
Sensors and Actuators B Chemical,
Год журнала:
2025,
Номер
unknown, С. 137856 - 137856
Опубликована: Апрель 1, 2025
Язык: Английский
Nanochannel confined graphene quantum dots/platinum nanoparticles boosts electrochemiluminescence of luminal-O2 system for sensitive immunoassay
Talanta,
Год журнала:
2024,
Номер
285, С. 127223 - 127223
Опубликована: Ноя. 20, 2024
Язык: Английский
Sensitive detection of gallic acid in food by electrochemical sensor fabricated by integrating nanochannel film with nanocarbon nanocomposite
Frontiers in Nutrition,
Год журнала:
2024,
Номер
11
Опубликована: Ноя. 12, 2024
Sensitive
detection
of
gallic
acid
(GA)
in
foods
is
great
significance
for
assessing
the
antioxidant
properties
products
and
ensuring
consumer
health.
In
this
work,
a
simple
electrochemical
sensor
was
conveniently
fabricated
by
integrating
vertically-ordered
mesoporous
silica
film
(VMSF)
with
electrochemically
reduced
graphene
oxide
(ErGO)
nitrogen
quantum
dots
(NGQDs)
nanocomposite,
enabling
sensitive
GA
food
sample.
A
water-soluble
mixture
(GO)
NGQDs
drop-cast
onto
common
carbon
electrode,
glassy
electrode
(GCE),
followed
rapid
growth
VMSF
using
an
assisted
self-assembly
method
(EASA).
The
negative
voltage
applied
during
facilitated
situ
reduction
GO
to
ErGO.
synergistic
effects
ErGO,
NGQDs,
nanochannels
led
nearly
tenfold
enhancement
signal
compared
that
obtained
on
electrodes
modified
either
ErGO
or
alone.
realized
linear
concentration
range
from
0.1
10
μM,
100
μM.
limit
(LOD),
determined
based
signal-to-noise
ratio
three
(
S
/
N
=
3),
found
be
81
nM.
Combined
size-exclusion
property
VMSF,
demonstrated
high
selectivity,
making
it
suitable
samples.
Язык: Английский