Sensitive detection of gallic acid in food by electrochemical sensor fabricated by integrating nanochannel film with nanocarbon nanocomposite DOI Creative Commons
Jiasheng Li,

Jiahui Lin,

Tao Luo

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Nov. 12, 2024

Sensitive detection of gallic acid (GA) in foods is great significance for assessing the antioxidant properties products and ensuring consumer health. In this work, a simple electrochemical sensor was conveniently fabricated by integrating vertically-ordered mesoporous silica film (VMSF) with electrochemically reduced graphene oxide (ErGO) nitrogen quantum dots (NGQDs) nanocomposite, enabling sensitive GA food sample. A water-soluble mixture (GO) NGQDs drop-cast onto common carbon electrode, glassy electrode (GCE), followed rapid growth VMSF using an assisted self-assembly method (EASA). The negative voltage applied during facilitated situ reduction GO to ErGO. synergistic effects ErGO, NGQDs, nanochannels led nearly tenfold enhancement signal compared that obtained on electrodes modified either ErGO or alone. realized linear concentration range from 0.1 10 μM, 100 μM. limit (LOD), determined based signal-to-noise ratio three ( S / N = 3), found be 81 nM. Combined size-exclusion property VMSF, demonstrated high selectivity, making it suitable samples.

Language: Английский

Sensitive Detection of Biomarker in Gingival Crevicular Fluid Based on Enhanced Electrochemiluminescence by Nanochannel-Confined Co3O4 Nanocatalyst DOI Creative Commons
Changfeng Zhu,

Yujiao Zhao,

Jiyang Liu

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(1), P. 63 - 63

Published: Jan. 19, 2025

The sensitive detection of inflammatory biomarkers in gingival crevicular fluid (GCF) is highly desirable for the evaluation periodontal disease. Luminol-based electrochemiluminescence (ECL) immunosensors offer a promising approach fast and convenient biomarkers. However, luminol’s low ECL efficiency under neutral conditions remains challenge. This study developed an immunosensor by engineering immunorecognition interface on outer surface mesoporous silica nanochannel film (SNF) confining Co3O4 nanocatalyst within SNF nanochannels to improve luminol efficiency. was grown indium tin oxide (ITO) electrode using simple Stöber solution growth method. A successfully confined through situ electrodeposition, confirmed X-ray photoelectron spectroscopy (XPS) electrochemical measurements. demonstrated excellent electrocatalytic activity, effectively enhancing H2O2 oxidation boosting signal conditions. Using interleukin-6 (IL-6) as proof-of-concept demonstration, epoxy functionalization enabled covalent immobilization capture antibodies, forming specific interface. IL-6 binding induced immunocomplex formation, which reduced allowed quantitative detection. showed linear range from 1 fg mL−1 10 ng mL−1, with limit (LOD) 0.64 mL−1. It also good selectivity anti-interference capabilities, enabling successful artificial GCF samples.

Language: Английский

Citations

4
Enhanced electrochemiluminescence of luminol at neutral medium using nanochannel-confined Co3O4 nanozyme for highly sensitive detection of tumor biomarker DOI

Jiayi Wu,

Le Wang, Hongxin Wang

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112903 - 112903

Published: Jan. 1, 2025

Language: Английский

Citations

1
Homogeneous Aptasensor with Electrochemical and Electrochemiluminescence Dual Detection Channels Enabled by Nanochannel-Based Probe Enrichment and DNase I Cleavage for Tumor Biomarker Detection DOI Creative Commons
Jiong Gao, Shiyue Zhang, Fengna Xi

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(3), P. 746 - 746

Published: Feb. 6, 2025

Homogeneous aptasensors that eliminate the need for probe labeling or immobilization hold significant potential rapid detection of tumor biomarkers. Herein, a homogeneous aptasensor with electrochemical (EC) and electrochemiluminescence (ECL) dual channels was developed by integrating nanochannel-based enrichment DNase I cleavage selective biomarker, carbohydrate antigen 125 (CA125). A two-dimensional (2D) composite prepared assembling CA125-specific aptamer cationic tris(2,2′-bipyridyl)Ru(II) (Ru(bpy)32+), which exhibited both EC ECL properties, onto graphene oxide (GO) nanosheets (Ru(bpy)32+/Apt@GO). vertically ordered mesoporous silica film (VMSF) ultrasmall, uniform, aligned nanochannel arrays rapidly grown on inexpensive disposable indium tin (ITO) electrode, forming interface. Due to size exclusion effect ultrasmall nanochannels in VMSF, Ru(bpy)32+/Apt@GO unable penetrate nanochannels, resulting no detectable Ru(bpy)32+ signal electrode. Upon specific recognition CA125 aptamer, an aptamer-CA125 complex formed subsequently detached from GO. then cleaved complex, releasing allowing dissociate into solution. This enzymatic enabled re-enter binding cycle, amplifying release The electrostatic adsorption VMSF significantly enhanced signals. constructed linear range 0.1 U/mL 100 ng/mL, limit (LOD) 91 mU/mL. For detection, detected over 0.001 U/mL, LOD as low 0.4 demonstrated excellent selectivity successfully applied dual-mode EC/ECL fetal bovine serum samples.

Language: Английский

Citations

1
A sensor based on Mo, N, S-doped interconnected porous carbon spheres material for simultaneous determination of hydroquinone and catechol DOI

Shoufeng Jiao,

J. J. Tang, Xiang Li

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113065 - 113065

Published: Feb. 1, 2025

Language: Английский

Citations

0
Highly sensitive electrochemical immunosensor based on methylene blue-reduced graphene oxide nanocomposites as signal probes for IL-6 detection in gingival crevicular fluid samples DOI Creative Commons
Changfeng Zhu, Hongxin Wang, Jiyang Liu

et al.

Frontiers in Chemistry, Journal Year: 2025, Volume and Issue: 13

Published: April 2, 2025

As an important inflammatory cytokine, interleukin-6 (IL-6) can mediate the entire pathological process of periodontitis and is closely associated with degree inflammation. Therefore, it critical to develop convenient quantitative methods for monitoring IL-6 quantity in gingival crevicular fluid. In this study, methylene blue (MB)-decorated reduced graphene oxide (rGO) employed as signal probe further support antibody-enabling specific recognition IL-6. Due π-π stacking electrostatic interactions, rGO-MB nanocomposites be stably obtained. rGO good conductivity large surface area characteristics promotes redox signals MB on glassy carbon electrode (GCE). addition, through simple situ self-polymerization dopamine, polydopamine (PDA) obtained not only directly used a biological crosslinking agent covalent immobilization anti-IL-6 antibody but also regarded protective layer enhance stability GCE surface. Such designed PDA/rGO-MB/GCE-based immunosensor enables binding produces decreased electrochemical MB, realizing selective sensitive measurement Consequently, our fabricated has excellent linear relationship ranging from 1 pg/mL 100 ng/mL, limit detection low 0.48 pg/mL. Moreover, as-prepared sensing strategy shows accurate fluid samples.

Language: Английский

Citations

0
A dual-functional antibiofouling and signal amplification sensing platform enabling accurate analysis in complicated biological samples DOI
Xue Fan, Tongtong Zhang, Shuhao Wang

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137856 - 137856

Published: April 1, 2025

Language: Английский

Citations

0
Nanochannel confined graphene quantum dots/platinum nanoparticles boosts electrochemiluminescence of luminal-O2 system for sensitive immunoassay DOI
Yucheng Zhou, Chaoyan Zhang, Jiyang Liu

et al.

Talanta, Journal Year: 2024, Volume and Issue: 285, P. 127223 - 127223

Published: Nov. 20, 2024

Language: Английский

Citations

1
Sensitive detection of gallic acid in food by electrochemical sensor fabricated by integrating nanochannel film with nanocarbon nanocomposite DOI Creative Commons
Jiasheng Li,

Jiahui Lin,

Tao Luo

et al.

Frontiers in Nutrition, Journal Year: 2024, Volume and Issue: 11

Published: Nov. 12, 2024

Sensitive detection of gallic acid (GA) in foods is great significance for assessing the antioxidant properties products and ensuring consumer health. In this work, a simple electrochemical sensor was conveniently fabricated by integrating vertically-ordered mesoporous silica film (VMSF) with electrochemically reduced graphene oxide (ErGO) nitrogen quantum dots (NGQDs) nanocomposite, enabling sensitive GA food sample. A water-soluble mixture (GO) NGQDs drop-cast onto common carbon electrode, glassy electrode (GCE), followed rapid growth VMSF using an assisted self-assembly method (EASA). The negative voltage applied during facilitated situ reduction GO to ErGO. synergistic effects ErGO, NGQDs, nanochannels led nearly tenfold enhancement signal compared that obtained on electrodes modified either ErGO or alone. realized linear concentration range from 0.1 10 μM, 100 μM. limit (LOD), determined based signal-to-noise ratio three ( S / N = 3), found be 81 nM. Combined size-exclusion property VMSF, demonstrated high selectivity, making it suitable samples.

Language: Английский

Citations

0