JACC Basic to Translational Science,
Год журнала:
2023,
Номер
8(8), С. 1028 - 1039
Опубликована: Март 29, 2023
Calcific
aortic
valve
stenosis
(CAVS)
is
a
widespread
valvular
heart
disease
affecting
people
in
aging
societies,
primarily
characterized
by
fibrosis,
inflammation,
and
progressive
calcification,
leading
to
orifice
stenosis.
Understanding
the
factors
associated
with
CAVS
onset
progression
crucial
develop
effective
future
pharmaceutical
therapies.
In
CAVS,
native
extracellular
matrix
proteins
modifications,
play
significant
role
calcification
vitro
vivo.
This
work
aimed
review
evidence
on
alterations
of
structural
involved
development
during
highlight
its
link
deregulated
biomechanical
function.
Experimental & Molecular Medicine,
Год журнала:
2024,
Номер
56(3), С. 527 - 548
Опубликована: Март 5, 2024
Abstract
Conventional
tumor
models
have
critical
shortcomings
in
that
they
lack
the
complexity
of
human
stroma.
The
heterogeneous
stroma
is
a
central
compartment
microenvironment
(TME)
must
be
addressed
cancer
research
and
precision
medicine.
To
fully
model
stroma,
deconstruction
reconstruction
tissues
been
suggested
as
new
approaches
for
vitro
modeling.
In
this
review,
we
summarize
heterogeneity
tumor-associated
stromal
cells
general
used
to
isolate
patient-specific
from
tissue;
also
address
effect
procedure
on
characteristics
primary
cells.
Finally,
perspectives
future
reconstructed
are
discussed,
with
an
emphasis
essential
prerequisites
developing
authentic
humanized
models.
Current Opinion in Cell Biology,
Год журнала:
2024,
Номер
88, С. 102355 - 102355
Опубликована: Апрель 16, 2024
Integrin-mediated
cell
adhesion
is
essential
for
migration,
mechanotransduction
and
tissue
integrity.
In
vivo,
these
processes
are
regulated
by
complex
physicochemical
signals
from
the
extracellular
matrix
(ECM).
These
nuanced
cues,
including
molecular
composition,
rigidity
topology,
call
sophisticated
systems
to
faithfully
explore
behaviour.
Here,
we
discuss
recent
methodological
advances
in
cell-ECM
research
compile
a
toolbox
of
techniques
that
expect
shape
this
field
future.
We
outline
breakthroughs
facilitating
transition
rigid
2D
substrates
more
dynamic
3D
systems,
as
well
super-resolution
imaging
an
in-depth
understanding
nanostructure.
Selected
methods
exemplified
with
relevant
biological
findings
underscore
their
applicability
research.
new
"toolbox"
will
allow
closer
approximation
vitro
experimental
setups
vivo
conditions,
providing
deeper
insights
into
physiological
pathophysiological
associated
adhesion.
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(36)
Опубликована: Апрель 18, 2024
Abstract
Decellularized
small
intestine
submucosa
(dSIS)
is
a
promising
biomaterial
for
promoting
tissue
regeneration.
Isolated
from
the
submucosal
layer
of
animal
jejunum,
SIS
rich
in
extracellular
matrix
(ECM)
proteins,
including
collagen,
laminin,
and
fibronectin.
Following
mild
decellularization,
dSIS
becomes
an
acellular
that
supports
cell
adhesion,
proliferation,
differentiation.
Conventional
usually
obtained
by
thermal
crosslinking,
which
yields
soft
scaffold
with
low
stability.
To
address
these
challenges,
modified
methacrylate
groups
photocrosslinking
into
stable
hydrogels.
However,
has
not
been
clickable
handles
orthogonal
crosslinking.
Here,
development
norbornene‐modified
dSIS,
named
dSIS‐NB,
via
reacting
amine
carbic
anhydride
acidic
aqueous
reaction
conditions
reported.
Using
triethylamine
(TEA)
as
base
catalyst,
high
degrees
NB
substitution
on
are
obtained.
In
addition
to
describing
synthesis
its
adaptability
hydrogel
crosslinking
cancer
vascular
engineering
explored.
Impressively,
compared
physically
crosslinked
collagen
matrices,
orthogonally
dSIS‐NB
hydrogels
supported
rapid
dissemination
cells
superior
vasculogenic
angiogenic
properties.
also
exploited
versatile
bioink
3D
bioprinting.
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 25, 2024
Abstract
Cell
spheroids
(esp.
organoids)
as
3D
culture
platforms
are
popular
models
for
representing
cell–cell
and
cell–extracellular
matrix
(ECM)
interactions,
bridging
the
gap
between
2D
cell
cultures
natural
tissues.
with
spatially
organized
multiple
types
preferred
gaining
comprehensive
insights
into
tissue
pathophysiology
constructing
in
vitro
tissues
disease
because
of
complexities
In
recent
years,
an
assembly
strategy
using
(or
living
building
blocks
has
been
developed
to
construct
complex
spatial
organization.
Here,
a
overview
advances
multispheroid
studies
is
provided.
The
different
mechanisms
techniques,
i.e.,
automated
directed
assembly,
noncontact
remote
programmed
self‐assembly,
introduced.
processing
steps,
advantages,
technical
limitations
existing
methodologies
summarized.
Applications
strategies
modeling,
drug
screening,
engineering,
organogenesis
reviewed.
Finally,
this
review
concludes
by
emphasizing
persistent
issues
future
perspectives,
encouraging
researchers
adopt
techniques
generating
advanced
that
better
resemble
real
Annals of the Rheumatic Diseases,
Год журнала:
2023,
Номер
82(11), С. 1474 - 1486
Опубликована: Июль 21, 2023
Activation
of
fibroblasts
is
a
hallmark
fibrotic
processes.
Besides
cytokines
and
growth
factors,
are
regulated
by
the
extracellular
matrix
environment
through
receptors
such
as
integrins,
which
transduce
biochemical
mechanical
signals
enabling
cells
to
mount
appropriate
responses
according
biological
demands.
The
aim
this
work
was
investigate
in
vivo
role
collagen-fibroblast
interactions
for
regulating
fibroblast
functions
fibrosis.Triple
knockout
(tKO)
mice
with
combined
ablation
integrins
α1β1,
α2β1
α11β1
were
created
address
significance
integrin-mediated
cell-collagen
communication.
Properties
primary
dermal
lacking
collagen-binding
delineated
vitro.
Response
tKO
skin
bleomycin
induced
challenge
assessed.Triple
integrin-deficient
develop
normally,
transiently
smaller
reveal
mild
alterations
mechanoresilience
skin.
Fibroblasts
from
these
culture
show
defects
cytoskeletal
architecture,
traction
stress
generation,
production
organisation.
Ablation
three
leads
increased
levels
discoidin
domain
receptor
2,
an
alternative
recognising
collagens
However,
overexpression
fails
compensate
adhesion
spreading
on
collagen
substrates
Mice
severely
attenuated
response
impaired
mechanotransduction,
reduced
organisation.The
data
provide
evidence
crucial
force
generation
differentiation
vitro
deposition
tissue
remodelling
vivo.
Targeting
fibroblast-collagen
might
represent
promising
therapeutic
approach
regulate
connective
diseases.
Nature Cell Biology,
Год журнала:
2023,
Номер
25(12), С. 1787 - 1803
Опубликована: Окт. 30, 2023
Invadosomes
and
caveolae
are
mechanosensitive
structures
that
implicated
in
metastasis.
Here,
we
describe
a
unique
juxtaposition
of
caveola
clusters
matrix
degradative
invadosomes
at
contact
sites
between
the
plasma
membrane
cancer
cells
constricting
fibrils
both
2D
3D
type
I
collagen
environments.
Preferential
association
straight
segments
fibrils,
bent
was
observed
along
with
remodelling.
Caveola
recruitment
precedes
is
required
for
invadosome
formation
activity.
Reciprocally,
disruption
results
accumulation
fibril-associated
caveolae.
Moreover,
receptor
β1
integrin
co-localize
integrins
control
to
fibrils.
In
turn,
mediate
clearance
uptake
an
invadosome-dependent
collagen-cleavage-dependent
mechanism.
Our
data
reveal
reciprocal
interplay
coordinates
adhesion
proteolytic
remodelling
confining
support
tumour
cell
dissemination.
Biosensors,
Год журнала:
2023,
Номер
13(10), С. 905 - 905
Опубликована: Сен. 24, 2023
The
increasing
popularity
of
3D
cell
culture
models
is
being
driven
by
the
demand
for
more
in
vivo-like
conditions
with
which
to
study
biochemistry
and
biomechanics
numerous
biological
processes
health
disease.
Spheroids
organoids
are
platforms
that
self-assemble
regenerate
from
stem
cells,
tissue
progenitor
cells
or
lines,
show
great
potential
studying
development
regeneration.
Organ-on-a-chip
approaches
can
be
used
achieve
spatiotemporal
control
over
biochemical
biomechanical
signals
promote
growth
differentiation.
These
model
systems
engineered
serve
as
disease
drug
screens.
While
methods
have
been
developed
support
these
structures,
challenges
remain
completely
recapitulate
cell–cell
cell–matrix
interactions
occurring
vivo.
Understanding
how
forces
influence
functions
will
require
precise
tools
measure
such
forces,
well
a
better
understanding
mechanobiology
interactions.
Biosensors
prove
powerful
measuring
both
contexts,
thereby
leading
mechanical
at
cellular
levels.
Here,
we
discussed
biosensors
mechanobiological
research
coupled
develop
accurate,
physiologically
relevant
development,
function,
malfunction
disease,
avenues
intervention.
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease,
Год журнала:
2024,
Номер
1870(3), С. 167042 - 167042
Опубликована: Янв. 29, 2024
Inflammatory
bowel
disease
(IBD)
encompasses
a
set
of
chronic
inflammatory
conditions,
namely
Crohn's
and
ulcerative
colitis.
Despite
all
advances
in
the
management
IBD,
definitive
cure
is
not
available,
largely
due
to
lack
holistic
understanding
its
etiology
pathophysiology.
Several
vitro,
vivo,
ex
vivo
models
have
been
developed
over
past
few
decades
order
abbreviate
remaining
gaps.
The
establishment
reliable
predictable
vitro
intestinal
inflammation
may
indeed
provide
valuable
tools
expedite
validate
development
therapies
for
IBD.
Three-dimensional
(3D)
more
accurate
representation
different
layers
intestine,
contributing
stronger
impact
on
drug
screening
research
inflammation,
bridging
gap
between
research.
This
work
provides
critical
overview
state-of-the-art
existing
3D
discusses
challenges,
providing
insights
possible
pathways
towards
achieving
IBD
mimetic
models.
We
also
address
some
main
challenges
faced
by
implementing
cell
culture
while
bearing
mind
clinical
translational
aspects.