Two‐Photon Laser Printing to Mechanically Stimulate Multicellular Systems in 3D DOI Creative Commons
Federico Colombo, Mohammadreza Taale, Fereydoon Taheri

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(19)

Published: March 4, 2024

Abstract Biological activities take place in 3D environments, where cells interact various directions a defined, often microstructured, space. A sub‐millimeter‐sized stretching device is developed to mechanically stimulate structurally restricted, soft multicellular microenvironment investigate the effect of defined cyclic mechanical forces on system. It consists multi‐material microstructure made Polydimethylsiloxane (PDMS) and gelatine‐based hydrogel, which printed using 2‐photon polymerization (2PP) method. The structures are first characterized microscopically study different printing parameters. Using 2PP, organotypic cell cultures then directly into hydrogel create true culture systems. These systems stimulated with cantilever by indenting at positions. change morphology actin orientation when exposed stretch, even within short timescales 30 min. As proof concept, Medaka retinal organoid encapsulated same structure demonstrate that preformed organoids can be this results highlight capability 2PP for manufacturing multifunctional devices control micrometer resolution thus mimic stresses as they occur vivo.

Language: Английский

Rebuilding the microenvironment of primary tumors in humans: a focus on stroma DOI Creative Commons
Siwon Mun, Hyun Jin Lee,

Pilnam Kim

et al.

Experimental & Molecular Medicine, Journal Year: 2024, Volume and Issue: 56(3), P. 527 - 548

Published: March 5, 2024

Abstract Conventional tumor models have critical shortcomings in that they lack the complexity of human stroma. The heterogeneous stroma is a central compartment microenvironment (TME) must be addressed cancer research and precision medicine. To fully model stroma, deconstruction reconstruction tissues been suggested as new approaches for vitro modeling. In this review, we summarize heterogeneity tumor-associated stromal cells general used to isolate patient-specific from tissue; also address effect procedure on characteristics primary cells. Finally, perspectives future reconstructed are discussed, with an emphasis essential prerequisites developing authentic humanized models.

Language: Английский

Citations

12
Novel tools to study cell-ECM interactions, cell adhesion dynamics and migration DOI Creative Commons
Michal Dibus,

Omkar Joshi,

Johanna Ivaska

et al.

Current Opinion in Cell Biology, Journal Year: 2024, Volume and Issue: 88, P. 102355 - 102355

Published: April 16, 2024

Integrin-mediated cell adhesion is essential for migration, mechanotransduction and tissue integrity. In vivo, these processes are regulated by complex physicochemical signals from the extracellular matrix (ECM). These nuanced cues, including molecular composition, rigidity topology, call sophisticated systems to faithfully explore behaviour. Here, we discuss recent methodological advances in cell-ECM research compile a toolbox of techniques that expect shape this field future. We outline breakthroughs facilitating transition rigid 2D substrates more dynamic 3D systems, as well super-resolution imaging an in-depth understanding nanostructure. Selected methods exemplified with relevant biological findings underscore their applicability research. new "toolbox" will allow closer approximation vitro experimental setups vivo conditions, providing deeper insights into physiological pathophysiological associated adhesion.

Language: Английский

Citations

10
Photo‐Responsive Decellularized Small Intestine Submucosa Hydrogels DOI
Van Thuy Duong, Han Nguyen, Ngoc Ha Luong

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(36)

Published: April 18, 2024

Abstract Decellularized small intestine submucosa (dSIS) is a promising biomaterial for promoting tissue regeneration. Isolated from the submucosal layer of animal jejunum, SIS rich in extracellular matrix (ECM) proteins, including collagen, laminin, and fibronectin. Following mild decellularization, dSIS becomes an acellular that supports cell adhesion, proliferation, differentiation. Conventional usually obtained by thermal crosslinking, which yields soft scaffold with low stability. To address these challenges, modified methacrylate groups photocrosslinking into stable hydrogels. However, has not been clickable handles orthogonal crosslinking. Here, development norbornene‐modified dSIS, named dSIS‐NB, via reacting amine carbic anhydride acidic aqueous reaction conditions reported. Using triethylamine (TEA) as base catalyst, high degrees NB substitution on are obtained. In addition to describing synthesis its adaptability hydrogel crosslinking cancer vascular engineering explored. Impressively, compared physically crosslinked collagen matrices, orthogonally dSIS‐NB hydrogels supported rapid dissemination cells superior vasculogenic angiogenic properties. also exploited versatile bioink 3D bioprinting.

Language: Английский

Citations

9
Micro Immune Response On-chip (MIRO) models the tumour-stroma interface for immunotherapy testing DOI Creative Commons
Alice Perucca,

Andrea Gómez Llonín,

Oriol Mañé Benach

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 3, 2025

Language: Английский

Citations

1
3D Multispheroid Assembly Strategies towards Tissue Engineering and Disease Modeling DOI

Tong Zhu,

Yan Hu, Haitao Cui

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: unknown

Published: June 25, 2024

Abstract Cell spheroids (esp. organoids) as 3D culture platforms are popular models for representing cell–cell and cell–extracellular matrix (ECM) interactions, bridging the gap between 2D cell cultures natural tissues. with spatially organized multiple types preferred gaining comprehensive insights into tissue pathophysiology constructing in vitro tissues disease because of complexities In recent years, an assembly strategy using (or living building blocks has been developed to construct complex spatial organization. Here, a overview advances multispheroid studies is provided. The different mechanisms techniques, i.e., automated directed assembly, noncontact remote programmed self‐assembly, introduced. processing steps, advantages, technical limitations existing methodologies summarized. Applications strategies modeling, drug screening, engineering, organogenesis reviewed. Finally, this review concludes by emphasizing persistent issues future perspectives, encouraging researchers adopt techniques generating advanced that better resemble real

Language: Английский

Citations

8
Trends in 3D models of inflammatory bowel disease DOI Creative Commons
Bárbara Ferreira, Andreia S. Barros, Catarina Leite Pereira

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2024, Volume and Issue: 1870(3), P. 167042 - 167042

Published: Jan. 29, 2024

Inflammatory bowel disease (IBD) encompasses a set of chronic inflammatory conditions, namely Crohn's and ulcerative colitis. Despite all advances in the management IBD, definitive cure is not available, largely due to lack holistic understanding its etiology pathophysiology. Several vitro, vivo, ex vivo models have been developed over past few decades order abbreviate remaining gaps. The establishment reliable predictable vitro intestinal inflammation may indeed provide valuable tools expedite validate development therapies for IBD. Three-dimensional (3D) more accurate representation different layers intestine, contributing stronger impact on drug screening research inflammation, bridging gap between research. This work provides critical overview state-of-the-art existing 3D discusses challenges, providing insights possible pathways towards achieving IBD mimetic models. We also address some main challenges faced by implementing cell culture while bearing mind clinical translational aspects.

Language: Английский

Citations

6
Coordinated in confined migration: crosstalk between the nucleus and ion channel-mediated mechanosensation DOI
Panagiotis Mistriotis, Emily Wisniewski,

R. Bishwa

et al.

Trends in Cell Biology, Journal Year: 2024, Volume and Issue: 34(10), P. 809 - 825

Published: Jan. 29, 2024

Language: Английский

Citations

6
Ablation of integrin-mediated cell–collagen communication alleviates fibrosis DOI
Mugdha Sawant, Fang Wang, Janis Koester

et al.

Annals of the Rheumatic Diseases, Journal Year: 2023, Volume and Issue: 82(11), P. 1474 - 1486

Published: July 21, 2023

Activation of fibroblasts is a hallmark fibrotic processes. Besides cytokines and growth factors, are regulated by the extracellular matrix environment through receptors such as integrins, which transduce biochemical mechanical signals enabling cells to mount appropriate responses according biological demands. The aim this work was investigate in vivo role collagen-fibroblast interactions for regulating fibroblast functions fibrosis.Triple knockout (tKO) mice with combined ablation integrins α1β1, α2β1 α11β1 were created address significance integrin-mediated cell-collagen communication. Properties primary dermal lacking collagen-binding delineated vitro. Response tKO skin bleomycin induced challenge assessed.Triple integrin-deficient develop normally, transiently smaller reveal mild alterations mechanoresilience skin. Fibroblasts from these culture show defects cytoskeletal architecture, traction stress generation, production organisation. Ablation three leads increased levels discoidin domain receptor 2, an alternative recognising collagens However, overexpression fails compensate adhesion spreading on collagen substrates Mice severely attenuated response impaired mechanotransduction, reduced organisation.The data provide evidence crucial force generation differentiation vitro deposition tissue remodelling vivo. Targeting fibroblast-collagen might represent promising therapeutic approach regulate connective diseases.

Language: Английский

Citations

16
A mechanosensitive caveolae–invadosome interplay drives matrix remodelling for cancer cell invasion DOI Creative Commons
Pedro Monteiro, David Remy,

Eline Lemerle

et al.

Nature Cell Biology, Journal Year: 2023, Volume and Issue: 25(12), P. 1787 - 1803

Published: Oct. 30, 2023

Invadosomes and caveolae are mechanosensitive structures that implicated in metastasis. Here, we describe a unique juxtaposition of caveola clusters matrix degradative invadosomes at contact sites between the plasma membrane cancer cells constricting fibrils both 2D 3D type I collagen environments. Preferential association straight segments fibrils, bent was observed along with remodelling. Caveola recruitment precedes is required for invadosome formation activity. Reciprocally, disruption results accumulation fibril-associated caveolae. Moreover, receptor β1 integrin co-localize integrins control to fibrils. In turn, mediate clearance uptake an invadosome-dependent collagen-cleavage-dependent mechanism. Our data reveal reciprocal interplay coordinates adhesion proteolytic remodelling confining support tumour cell dissemination.

Language: Английский

Citations

16
Impact of mechanical cues on key cell functions and cell-nanoparticle interactions DOI Creative Commons

Petra Elblová,

Mariia Lunová, A. Dejneka

et al.

Discover Nano, Journal Year: 2024, Volume and Issue: 19(1)

Published: June 22, 2024

Abstract In recent years, it has been recognized that mechanical forces play an important regulative role in living organisms and possess a direct impact on crucial cell functions, ranging from growth to maintenance of tissue homeostasis. Advancements mechanobiology have revealed the profound signals diverse cellular responses are type specific. Notably, numerous studies elucidated pivotal different cues as regulatory factors influencing various processes, including spreading, locomotion, differentiation, proliferation. Given these insights, is unsurprising cells regulated by physical intricately linked modulation nanoparticle uptake kinetics processing. This complex interplay underscores significance understanding microenvironment shaping behaviors and, consequently, how interact with process nanoparticles. Nevertheless, our knowledge localized affect internalization processing nanoparticles remains rather limited. A significant gap exists literature concerning systematic analysis might bias interactions between cells. Hence, aim this review provide comprehensive critical existing regarding influence complicated dynamics cell-nanoparticle interactions. By addressing gap, we would like contribute detailed

Language: Английский

Citations

5