Frontiers in Physiology,
Год журнала:
2020,
Номер
11
Опубликована: Апрель 3, 2020
Immunotherapy
is
a
relatively
new
treatment
regimen
for
cancer,
and
it
based
on
the
modulation
of
immune
system
to
battle
cancer.
Immunotherapies
can
be
classified
as
either
molecular
or
cell-based
immunotherapies,
both
types
have
demonstrated
promising
results
in
growing
number
cancers.
Indeed,
several
immunotherapies
representing
classes
are
already
approved
clinical
use
oncology.
While
spectacular
successes
been
reported,
particularly
so-called
checkpoint
inhibitors
certain
they
also
accompanied
by
variety
severe,
sometimes
life-threatening
side
effects.
Furthermore,
not
all
patients
respond
immunotherapy.
Hence,
there
need
more
research
render
these
therapeutics
efficacious,
widely
applicable,
safer
use.
Whole-body
vivo
imaging
technologies
that
interrogate
cancers
and/or
highly
beneficial
tools
during
immunotherapy
development
translation
clinic.
In
this
review,
we
explain
how
aid
anti-cancer
immunotherapies.
We
describe
principles
host
T-cells
adoptively
transferred
therapeutic
well
value
traceable
cancer
cell
models
development.
Our
emphasis
tracking
methodology,
including
important
aspects
caveats
specific
discuss
associated
experimental
design
parameters
such
type,
observation
times
intervals,
detection
sensitivity.
The
focus
preclinical
but
relevant
discussed.
Nature Communications,
Год журнала:
2020,
Номер
11(1)
Опубликована: Янв. 13, 2020
Abstract
Bright
monomeric
near-infrared
(NIR)
fluorescent
proteins
(FPs)
are
in
high
demand
as
protein
tags
for
multicolor
microscopy
and
vivo
imaging.
Here
we
apply
rational
design
to
engineer
a
complete
set
of
NIR
FPs,
which
the
brightest
genetically
encoded
probes.
We
demonstrate
that
enhanced
miRFP
series
combine
effective
brightness
mammalian
cells
state,
perform
well
both
nanometer-scale
imaging
with
diffraction
unlimited
stimulated
emission
depletion
(STED)
centimeter-scale
mice.
In
STED
achieve
~40
nm
resolution
live
cells.
living
mice
detect
~10
5
deep
tissues.
Using
spectrally
distinct
FP
variants,
two-color
live-cell
vivo.
Having
peaks
from
670
720
nm,
next
generation
miRFPs
should
become
versatile
probes
multiplexed
across
spatial
scales
different
modalities.
Chemical Society Reviews,
Год журнала:
2023,
Номер
52(4), С. 1189 - 1214
Опубликована: Янв. 1, 2023
The
genetically
encoded
fluorescent
sensors
convert
chemical
and
physical
signals
into
light.
They
are
powerful
tools
for
the
visualisation
of
physiological
processes
in
living
cells
freely
moving
animals.
protein
is
reporter
module
a
biosensor.
In
this
study,
we
first
review
history
full
emission
spectra
on
structural
basis.
Then,
discuss
design
Finally,
briefly
several
major
types
biosensors
that
currently
widely
used
based
their
molecular
targets,
which
may
be
useful
future
biosensors.
Nature Communications,
Год журнала:
2019,
Номер
10(1)
Опубликована: Янв. 11, 2019
From
a
single
domain
of
cyanobacteriochrome
(CBCR)
we
developed
near-infrared
(NIR)
fluorescent
protein
(FP),
termed
miRFP670nano,
with
excitation
at
645
nm
and
emission
670
nm.
This
is
the
first
CBCR-derived
NIR
FP
evolved
to
efficiently
bind
endogenous
biliverdin
chromophore
brightly
fluoresce
in
mammalian
cells.
miRFP670nano
monomer
molecular
weight
17
kDa
that
2-fold
smaller
than
bacterial
phytochrome
(BphP)-based
FPs
1.6-fold
GFP-like
FPs.
Crystal
structure
CBCR-based
reveals
basis
its
spectral
biochemical
properties.
Unlike
BphP-derived
FPs,
highly
stable
denaturation
degradation
can
be
used
as
an
internal
tag.
effective
FRET
donor
for
red-shifted
enabling
engineering
biosensors
spectrally
compatible
blue-green
optogenetic
tools.
unlocks
new
source
diverse
CBCR
templates
Frontiers in Cellular Neuroscience,
Год журнала:
2019,
Номер
13
Опубликована: Окт. 23, 2019
Understanding
how
neuronal
activity
patterns
in
the
brain
correlate
with
complex
behavior
is
one
of
primary
goals
modern
neuroscience.
Chemical
transmission
major
way
communication
between
neurons;
however,
traditional
methods
detection
neurotransmitter
and
neuromodulator
transients
mammalian
lack
spatiotemporal
precision.
Modern
fluorescent
biosensors
for
neurotransmitters
neuromodulators
allow
monitoring
chemical
vivo
millisecond
precision
single
cell
resolution.
Changes
biosensor
brightness
occur
upon
binding
can
be
detected
using
fiber
photometry,
stationary
microscopy
miniaturized
head-mounted
microscopes.
Biosensors
expressed
animal
adeno-associated
viral
vectors,
their
cell-specific
expression
achieved
Cre-recombinase
expressing
animals.
Although
initially
were
represented
by
glutamate
biosensors,
nowadays
GABA,
acetylcholine,
glycine,
norepinephrine
dopamine
are
available
as
well.
In
this
review,
we
overview
functioning
principles
existing
intensiometric
ratiometric
provide
brief
insight
into
variety
neurotransmitter-binding
proteins
from
bacteria,
plants
eukaryotes
including
G-protein
coupled
receptors,
which
may
serve
scaffolds.
We
next
describe
a
workflow
development
biosensors.
then
discuss
advanced
setups
functional
imaging
awake
freely
moving
conclude
providing
application
examples
studies
single-neuron
Sensors,
Год журнала:
2021,
Номер
21(3), С. 906 - 906
Опубликована: Янв. 29, 2021
The
purpose
of
this
work
is
to
provide
an
exhaustive
overview
the
emerging
biosensor
technologies
for
detection
analytes
interest
food,
environment,
security,
and
health.
Over
years,
biosensors
have
acquired
increasing
importance
in
a
wide
range
applications
due
synergistic
studies
various
scientific
disciplines,
determining
their
great
commercial
potential
revealing
how
nanotechnology
biotechnology
can
be
strictly
connected.
In
present
scenario,
increased
limit
sensitivity
unthinkable
until
few
years
ago.
most
widely
used
are
optical-based
devices
such
as
surface
plasmon
resonance
(SPR)-based
fluorescence-based
biosensors.
Here,
we
will
review
them
by
highlighting
progress
design
development
could
impact
our
daily
life.
