Journal of Drug Delivery Science and Technology, Год журнала: 2024, Номер unknown, С. 106189 - 106189
Опубликована: Сен. 1, 2024
Язык: Английский
Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)
Опубликована: Фев. 10, 2024
Abstract Extracellular vesicles have shown promising tissue recovery-promoting effects, making them increasingly sought-after for their therapeutic potential in wound treatment. However, traditional extracellular vesicle applications suffer from limitations such as rapid degradation and short maintenance during administration. To address these challenges, a growing body of research highlights the role hydrogels effective carriers sustained release, thereby facilitating healing. The combination with development 3D bioprinting create composite hydrogel systems boasting excellent mechanical properties biological activity, presenting novel approach to healing skin dressing. This comprehensive review explores remarkable hydrogels, specifically suited loading vesicles. We delve into diverse sources analyzing integration within formulations Different methods well bioprinting, adapted varying conditions construction strategies, are examined roles promoting results highlight vesicle-laden advanced tools field treatment, offering both support bioactive functions. By providing an in-depth examination various that can play healing, this sheds light on directions further development. Finally, we challenges associated application along emerging trends domain. discussion covers issues scalability, regulatory considerations, translation technology practical clinical settings. In conclusion, underlines significant contributions hydrogel-mediated therapy regeneration. It serves valuable resource researchers practitioners alike, fostering deeper understanding benefits, applications, involved utilizing Graphical abstract
Язык: Английский
Процитировано
21
Journal of Drug Delivery Science and Technology, Год журнала: 2025, Номер 106, С. 106696 - 106696
Опубликована: Фев. 6, 2025
Язык: Английский
Процитировано
1
Nano Today, Год журнала: 2024, Номер 55, С. 102169 - 102169
Опубликована: Янв. 26, 2024
Язык: Английский
Процитировано
7
International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 8211 - 8236
Опубликована: Авг. 1, 2024
Hypoxia, as a prominent feature of the tumor microenvironment, has profound impact on multicomponent changes within this environment. Under hypoxic conditions, malignant phenotype cells, variety cell types well intercellular communication and material exchange, undergo complex alterations. These provide significant prospects for exploring mechanisms development under different microenvironmental conditions devising therapeutic strategies. Exosomes secreted by cells stromal are integral components serving crucial mediators have consequently garnered increasing attention from researchers. This review focuses which promote release exosomes alter their encapsulated contents. It also examines effects derived immune other microenvironment. Additionally, we summarize current research progress potential clinical applications propose future directions in field.
Язык: Английский
Процитировано
7
Applied Materials Today, Год журнала: 2024, Номер 37, С. 102084 - 102084
Опубликована: Фев. 3, 2024
Extracellular vesicles (EVs) are biologically active nanoparticles secreted by cells, which have a variety of physiological functions, such as promoting cell proliferation, regulating immunity, anti-inflammation, and facilitating angiogenesis. However, the application EVs is limited defects low therapeutic efficiency, lack targeting, unstable effects. In recent years, various engineering methods been developed to enhance function EVs. addition, research on isolation mechanisms has also made significant progress. this paper, we review biogenesis, composition, techniques EVs, summarize potential natural well strategies for EV modification, highlight advances in tissue regeneration. Finally, discuss EVs' current problems future perspectives regenerative medicine.
Язык: Английский
Процитировано
6
Frontiers in Pharmacology, Год журнала: 2023, Номер 14
Опубликована: Сен. 8, 2023
Exosomes are small membrane vesicles of endocytic origin that produced by both tumor and normal cells can be found in physiological fluids like plasma cell culture supernatants. They include cytokines, growth factors, proteins, lipids, RNAs, metabolites important intercellular communication controllers several disorders. According to a vast amount research, exosomes could support or inhibit start diffusion variety solid hematological malignancies paracrine signaling. crucial therapeutic agents for illnesses, such as cancer autoimmune diseases. This review discusses the most current encouraging findings from vitro experimental vivo well scant number ongoing clinical trials, with focus on impact treatment malignancies. have great promise carriers medications, antagonists, genes, other materials incorporated into their core ways. also alter metabolism cells, activity immunologic effectors, non-coding all which microenvironment turn it pro-tumor an anti-tumor milieu. subject is covered review, looks at how contribute onset progression malignancies, importance diagnosing treating these conditions.
Язык: Английский
Процитировано
12
BMC Veterinary Research, Год журнала: 2025, Номер 21(1)
Опубликована: Янв. 21, 2025
Small extracellular vesicles (sEVs) derived from mesenchymal stem cells (MSCs) are recognized for their therapeutic potential in immune modulation and tissue repair, especially veterinary medicine. This study introduces an innovative sequential stimulation (IVES) technique, involving low-oxygen gas mixture preconditioning using vitro fertilization (IVFG) direct current electrical (ES20), to enhance the anti-inflammatory properties of sEVs canine adipose-derived MSCs (cAD-MSCs). Initial steps involved isolation comprehensive characterization cAD-MSCs, including morphology, gene expression, differentiation potentials, alongside validation protocol. IVFG, ES20, IVES were applied simultaneously with a control condition. Stimulated cAD-MSCs evaluated morphological changes, cell viability, expressions. Conditioned media collected purified sEV on Day1, Day2, Day3. To validate efficacy production, various analyses conducted, microscopic examination, surface marker assessment, zeta-potential measurement, protein quantification, nanoparticle tracking analysis, determination activity. We found that demonstrated non-cytotoxicity induced crucial genotypic changes associated production cAD-MSCs. Interestingly, IVFG influenced cellular adaptation, while ES20 hypoxia activation. By merging these stimulations, enhanced stability quality profiles. The cAD-MSC-derived exhibited activity lipopolysaccharide-induced RAW264.7 macrophages, emphasizing improved effectiveness without cytotoxicity or immunogenicity. These effects consistent across day 3 collection, indicating establishment effective protocol production. research established method positive impact characteristics stability, quality, not only contributes enhancement but also sheds light functional aspects interventions.
Язык: Английский
Процитировано
0
Tissue and Cell, Год журнала: 2025, Номер 93, С. 102749 - 102749
Опубликована: Янв. 23, 2025
Язык: Английский
Процитировано
0
Gels, Год журнала: 2025, Номер 11(3), С. 191 - 191
Опубликована: Март 8, 2025
Three-dimensional bioprinting is a new advance in tissue engineering and regenerative medicine. Bioprinting allows manufacturing three-dimensional (3D) structures that mimic tissues or organs. The bioinks used are mainly made of natural synthetic polymers must be biocompatible, printable, biodegradable. These may incorporate progenitor cells, favoring graft implantation regeneration injured tissues. However, the natures biomaterials, processes, lack vascularization, immune responses factors limit viability functionality implanted cells damaged limitations can addressed by incorporating extracellular vesicles (EV) into bioinks. Indeed, EV from have capacities, being similar to those their source cells. Therefore, combinations with biomaterials cell-free therapies. Likewise, they complement manufacture increasing viability, differentiation, ability incorporated Thus, main objective this review show how use 3D technology for application medicine these nanovesicles hydrogels as To end, latest advances derived vitro vivo studies been described. Together, high therapeutic potential strategy
Язык: Английский
Процитировано
0
Journal of Materials Chemistry B, Год журнала: 2023, Номер 11(43), С. 10263 - 10287
Опубликована: Янв. 1, 2023
Bioprinting, as a groundbreaking technology, enables the fabrication of biomimetic tissues and organs with highly complex structures, multiple cell types, mechanical heterogeneity, diverse functional gradients. With growing demand for organ transplantation limited number donors, bioprinting holds great promise addressing shortage by manufacturing completely organs. While complete remains distant goal, there has been considerable progress in development bioprinted transplantable regenerative medicine. This review article recapitulates current achievements 3D bioprinting, primarily encompassing five important human body (i.e., heart, kidneys, liver, pancreas, lungs). Challenges from cellular techniques, biomanufacturing technologies, maturation techniques are also deliberated broad application bioprinting. In addition, integration other cutting-edge technologies including machine learning, organoids, microfluidics is envisioned, which strives to offer reader prospect constructing
Язык: Английский
Процитировано
9