Argonaute-Based Nucleic Acid Detection Technology: Advantages, Current Status, Challenges, and Perspectives

ACS Sensors, Год журнала: 2024, Номер 9(11), С. 5665 - 5682

Опубликована: Ноя. 11, 2024

Rapid and accurate detection is a prerequisite for precise clinical diagnostics, ensuring food safety, facilitating biotechnological applications. The Argonaute system, as cutting-edge technique, has been successfully repurposed in biosensing beyond the CRISPR/Cas system (clustered regularly interspaced short palindromic repeats CRISPR-associated proteins), which extensively researched, but recognition of PAM sequences remains restricted. Argonaute, programmable target-activated nuclease, fabricating novel methods due to its unparalleled biological features. In this comprehensive review, we initially elaborate on current nucleic acid testing nucleases, followed by delving into structure nuclease activity system. advantages compared with are highlighted discussed. Furthermore, summarize applications Argonaute-based provide an in-depth analysis future perspectives challenges. Recent research demonstrated that innovative rapidly advancing technology can overcome limitations existing potentially replace them. summary, implementation integration other technologies hold promise developing customized intelligent across various aspects.

Язык: Английский

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Argonaute protein powered biosensing for pathogenic biosafety

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 305, С. 141321 - 141321

Опубликована: Фев. 19, 2025

Язык: Английский

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CRISPR/Cas and Argonaute-Based Biosensors for Nucleic acid Detection

Talanta, Год журнала: 2025, Номер 294, С. 128210 - 128210

Опубликована: Апрель 22, 2025

Язык: Английский

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A small library crRNA-enhanced CRISPR-Cas12a system for ultrasensitive point-of-care test of hantavirus M gene

Sensing and Bio-Sensing Research, Год журнала: 2025, Номер unknown, С. 100811 - 100811

Опубликована: Май 1, 2025

Язык: Английский

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A RT-ERA-CRISPR/Cas12a assay for rapid Point-of-Care duck hepatitis A virus detection

Poultry Science, Год журнала: 2025, Номер 104(8), С. 105316 - 105316

Опубликована: Май 23, 2025

Duck hepatitis A virus (DHAV) is a severe pathogen that threatens the duck industry. DHAV transmitted primarily through respiratory and gastrointestinal tracts. Therefore, developing accurate rapid diagnostic technologies crucial to prevent spread of this infectious disease. Currently, most widely used detection methods in clinical diagnosis include PCR, real-time PCR (RT-PCR) ELISA, which require specialized equipment or trained professionals. Isothermal amplification are often combined with lateral flow assays visual readout for detecting nucleic acids non-laboratory settings. CRISPR nucleases have accelerated development acid detection, increasing sensitivity higher degree. Here, we applied reverse transcription-enzymatic recombinase (RT-ERA) assisted by Cas12a-fluorescence assay Cas12a-lateral DHAV-1. Based on sequence DHAV-1, RT-ERA primers crRNAs were designed, different concentrations ssDNA/Cas12a/crRNA established optimize reaction. The LoD was 10 copies/μL, effectively differentiated DHAV-1 from other avian pathogens, exhibiting high specificity. Additionally, user-friendly can achieve point-of-care detection. Sixty-four samples tested compared quantitative (RT-PCR). This has significant potential applications, especially farms rural areas.

Язык: Английский

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Applications of CRISPR Technologies in Forestry and Molecular Wood Biotechnology

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(21), С. 11792 - 11792

Опубликована: Ноя. 2, 2024

Forests worldwide are under increasing pressure from climate change and emerging diseases, threatening their vital ecological economic roles. Traditional breeding approaches, while valuable, inherently slow limited by the long generation times existing genetic variation of trees. CRISPR technologies offer a transformative solution, enabling precise efficient genome editing to accelerate development climate-resilient productive forests. This review provides comprehensive overview applications in forestry, exploring its potential for enhancing disease resistance, improving abiotic stress tolerance, modifying wood properties, accelerating growth. We discuss mechanisms various systems, including base editing, prime multiplexing strategies. Additionally, we highlight recent advances overcoming key challenges such as reagent delivery plant regeneration, which crucial successful implementation also delve into ethical considerations using gene drive population-level alterations, well importance containment strategies mitigating risks. emphasizes need continued research, technological advancements, extensive long-term field trials, public engagement, responsible innovation fully harness power shaping sustainable future

Язык: Английский

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Trends in the use of Argonaute Proteins in Molecular Diagnosis

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер unknown, С. 118081 - 118081

Опубликована: Ноя. 1, 2024

Язык: Английский

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