Novel Insights Into Telocyte Presence and Distribution in the Cornea of the Laughing Dove (Spilopelia senegalensis) Using Immunofluorescence Analysis

Microscopy Research and Technique, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

ABSTRACT The eye of the laughing dove ( Spilopelia senegalensis ) is uniquely adapted to thrive in arid environments, withstanding airborne particles and harsh conditions, demonstrating its remarkable structural functional resilience. This study aimed explore cellular composition dove's cornea, focusing on telocytes—a cell type previously unreported avian corneas. work represents first demonstration telocytes cornea any bird species. We used hematoxylin eosin (H&E) staining comprehensively visualize corneal layers, while silver staining, methylene blue, toluidine blue immunofluorescence with telocyte‐specific markers (CD34, PDGFRα, vimentin) were utilized identify confirm their distribution. was shown consist five unique layers: epithelium, Bowman's layer, stroma, Descemet's membrane, endothelium. Telocytes detected using silver, stains as spindle‐shaped cells branched extensions known telopodes. Double for PDGFRα CD34 confirmed existence across all layers. In intraepithelial sparsely distributed, possibly contributing epithelial maintenance signaling. identified, likely supporting integrity intercellular interactions. stroma exhibited highest abundance telocytes, highlighting critical role extracellular matrix remodeling connectivity. contribute stability this basement endothelium, they appear play roles endothelial repair, fluid regulation, signaling processes. vimentin revealed distinct localization patterns. While identified expression confined predominantly superficial region. Vimentin specifically localized telopodes, more evenly strong both central bodies partial co‐expression suggests specialization within telopode structure long‐range Our findings underscore function, particularly contributions adaptation environmental conditions. not only identifies time but also highlights presence suggesting broader maintaining homeostasis

Язык: Английский

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A novel immunofluorescence study of Lingual Salivary Glands in the Egyptian Tortoise (Testudo kleinmanni) and its ecological significance

Tissue and Cell, Год журнала: 2024, Номер 90, С. 102517 - 102517

Опубликована: Авг. 10, 2024

Язык: Английский

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Anatomy, histology, and morphology of fish gills in relation to feeding habits: a comparative review of marine and freshwater species

BMC Zoology, Год журнала: 2025, Номер 10(1)

Опубликована: Фев. 7, 2025

Abstract This systematic review highlights the similarities and variations in gill morphology, histology, anatomical structure between differing fish species. The system consists of mainly four pairs arches most teleost fishes, such as sea bass, bream, grouper, red porgy, etc., while it three pufferfish striped-red mullet fish. However, Clarias gariepinus had five pairs, including an additional rudimentary fifth-gill arch. consisted arches, rakers, filaments, secondary lamellae with varied shapes hook, semilunar, L-shapes, crescentic shapes. Each arch carried two rows lateral medial, present fishes ( Mugil cephalus, Boops boops, Pagrus pagrus, Sparus aurata , European hake, Puffer fish, grey gurnard, bream). An row appears or (accessory) dusky grouper length shape rakers are related to feeding habits. longer, equal, more number developed than those medial rows, except at second third pufferfish, fourth pagrus . absent first Bagrus bayad carries structures, air-breathing dendritic organ catfish, located suprabranchial chamber caudodorsal gills composed main parts: small large ones originated by stems from fourth-gill respectively. interbranchial septum can be smooth, form a median crest (seabream), carry teeth spines (seabass, pufferfish). Four transversely raised areas on each side connected transverse lines caudal base tongue ) elevated part level third-gill Tilapia zilli ). Scanning electron microscopy explained micro-anatomical structures pavement cells, mucus cell openings, taste buds arch, grooves near filament side, their spines, heights types Histological findings revealed various superficial chloride mucous goblet basal epithelial cells. lymph space is situated within epithelia encompassed cells that resemble tenocytes. contains many immunological lymphocytes, granular leukocytes, rodlet comprises sensory known neuromasts hyaline cartilaginous support. underscores intricate relationship habits across marine freshwater highlighting importance understanding these for ecological, evolutionary, aquacultural applications

Язык: Английский

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Novel identification of telocytes in the skin of the desert tortoise (Testudo graeca): A correlative study using semithin section, transmission electron microscopy, and immunofluorescence

Research in Veterinary Science, Год журнала: 2025, Номер 191, С. 105689 - 105689

Опубликована: Май 6, 2025

Язык: Английский

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Comparative morphological analysis of telson and uropods in Penaeus canaliculatus (Olivier, 1811), Penaeus semisulcatus (De Haan, 1844), and Metapenaeus stebbingi (Nobili, 1904) using scanning electron microscopy and EDX analysis

Micron, Год журнала: 2024, Номер 181, С. 103636 - 103636

Опубликована: Апрель 1, 2024

Язык: Английский

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Macro‐ and micro‐morphological comparison of the detailed structure of the oral cavity roof in two different feeding habits marine fishes: Pagrus pagrus and Boops boops

Journal of Experimental Zoology Part A Ecological and Integrative Physiology, Год журнала: 2024, Номер 341(7), С. 783 - 797

Опубликована: Апрель 23, 2024

Abstract The feeding habits and habitats of fish influence the morphology oral cavity. This study used gross anatomy, light microscopy, scanning electron in addition to morphometric analysis, investigate anatomical characteristics cavity roof Pagrus pagrus Boops boops , which have different dietary habits. appeared U‐shaped divided into palate upper pharyngeal regions. lip P. was broad, while B. boops' small thin. Both species had a stratified squamous epithelium with an irregular shape folded surface. horseshoe‐shaped velum high middle part, its surface resembled sea waves obvious mucous‐secreting openings cilia many folds grooves between them. boops's thin as triangle pouch pointed cranial apex. both narrow front increased width backward until it ended. teeth two patches, separated by median longitudinal ridge anterior V‐shape separator. Meanwhile, they ball patch on sides separator middle. Because fed harder structures than their were reflected structure roof. carnivorous species, several rows sharp jaw teeth, thick spinous tubercles oblique transverse ridges, massive mucous glands. On other hand, omnivorous only one row few scattered oval filaments ridges.

Язык: Английский

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Novel Insights Into the Ultrastructural and Immunofluorescence Characteristics of Limb Skin in the Red‐Eared Slider Turtle (Trachemys scripta elegans)

Microscopy Research and Technique, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 7, 2024

The red-eared slider turtle, a species facing environmental challenges and habitat loss, exhibits complex skin architecture that is crucial for its adaptation survival. Our study aims to provide comprehensive characterization of the turtle's structure elucidate distribution localization various cellular components, with focus on understanding skin's role in ecological interactions. To achieve these goals, we employed light microscopy, transmission electron microscopy (TEM), immunofluorescence using 10 specific antibodies. forelimb displays large- moderate-sized scales variations color, including dark, yellow, gray hues, likely contributing camouflage protection. consists corneous material, epidermis, dermis, hypodermis. stratum basalis, spinosum, peri-corneous layer constitute three distinct layers epidermis. There are four types chromatophores, melanocytes located while melanophores, xanthophores, iridophores found within dermal layer. also well-developed peripheral nerves, blood vessels, subcutaneous muscles. Immunofluorescence staining further elucidates cells. E-cadherin CK14 strongly expressed epidermal layers, excluding material. surrounds keratinocyte cells facilitating cell-cell adhesion, present inside cells, their internal structural integrity. Sox10 CD117 identify chromatophore types, Melan-A specifically detecting only melanophores not labeling xanthophores iridophores. Tom20 used detect mitochondrial intensity skin, revealing high density mitochondria all especially compared Numerous telocytes, spindle-shaped extensions called telopods, detected dermis CD34, PDGFRα, vimentin. shows abundant myofibroblasts vascularization, numerous vessels α-SMA. This novel offers an in-depth examination limb through use antibodies, uncovering intricate interactions among components providing valuable insights into anatomical physiological adaptations. findings contribute better which may aid conservation management.

Язык: Английский

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Macroscopic, microscopic, and immunofluorescent characterization of the Greek tortoise (Testudo graeca graeca) oropharyngeal floor with concern to its feed adaptation as a herbivorous land reptile

Microscopy Research and Technique, Год журнала: 2024, Номер 87(10), С. 2385 - 2398

Опубликована: Май 29, 2024

Abstract The current investigation focuses on gross anatomy, light, and scanning electron microscopy (SEM) of the Testudo graeca oropharyngeal floor, with particular reference to immunofluorescence technique examine its tongue. T . floor showed many anatomical structures: lower rhamphotheca, paralingual ridge, alveolar tongue, laryngeal mound, glottis. rhamphotheca appeared as a V‐shaped jaw line highly serrated edge median tomium (beak). SEM observations lingual apex body rectangular conical filiform papillae porous surfaces taste pores. Meanwhile, root had two wings that carried different shapes: dagger‐shaped, conical, bifurcated, leaf‐like papillae, these lacked mound openings for mucus gland secretions. Light findings mucous glands in propria submucosa near mucosal surface apex. hyaline cartilaginous skeletons between skeletal muscles, were elongated filiform, fungiform papillae. lamina constituted core gland, they positive reaction periodic acid schiff (PAS) reagent. apical Under immunofluorescence, vimentin was detected bud cells, synaptophysin reacted buds nerve bundles. study Greek tortoise investigated herbivorous eating habits using large tongue differently shaped numerous glands. Research Highlights (T graeca) light revealed varied types shapes (rectangular or papillae), (filiform (dagger‐shaped, papillae). findings: slightly magenta‐red color PAS Vimentin gave buds.

Язык: Английский

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Analyzing the morphology and avian β-defensins genes (AvβD) expression in the small intestine of Cobb500 broiler chicks fed with sodium butyrate

BMC Veterinary Research, Год журнала: 2024, Номер 20(1)

Опубликована: Сен. 28, 2024

Язык: Английский

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Macro- and micro-anatomical investigation of the oropharyngeal roof of landform greek tortoise (Testudo graeca graeca) and semi-aquatic red-eared slider turtle (Trachemys scripta elegans)

BMC Veterinary Research, Год журнала: 2024, Номер 20(1)

Опубликована: Июль 11, 2024

Abstract The present investigation examined the oropharyngeal roof of two turtles having different feeding behaviors: landform Greek tortoise ( Testudo graeca ) primarily herbivores and semi-aquatic red-eared slider turtle Trachemys scripta elegans lives in freshwater that opportunistic omnivorous grossly by scanning light microscopes. Grossly, had a V-shaped consisting upper rhamphotheca, peri-palatine region, alveolar ridge, peripheral palatine median vomer, choanae, caudal part, pharynx. At same time, semilunar ridges, core band, SEM revealed appeared more straightforward. rhamphotheca is sharp, with premaxillary notch gives powerful bite for cutting to compensate absence teeth. Additionally, slider’s band interrupted single ridge appears spiky, pointed, longer as it needs chewing prey there are types teeth-like projections at its area food-crushing chewing. region numerous ridges folds provide roughness food processing. tortoises small-sized choanae choanal minimize openings when eating dusty grasses. Histologically, palate was rostrally thicker keratinized than caudally, characterized pair circumvallate-like papilla multiple mucous secretions, while slightly peri-choanal rest non-keratinized few openings. current found various structural adaptations behavior omnivore slide compared herbivorous turtle.

Язык: Английский

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