ACS Nano,
Год журнала:
2024,
Номер
18(49), С. 33505 - 33519
Опубликована: Дек. 2, 2024
The
development
of
novel
detection
technology
for
meat
species
authenticity
is
imperative.
Here,
we
developed
a
machine
learning-supported,
dual-channel
biosensor-in-microdroplet
platform
named
CC-drop
(CRISPR/Cas12a
digital
single-molecule
microdroplet
biosensor).
This
strategy
allowed
us
to
quickly
identify
and
analyze
animal-derived
components
in
foods.
biosensor
was
enabled
by
CRISPR/Cas12a-based
fluorescence
lighting-up
detection,
the
nucleic
acid
signals
can
be
recognized
Cas12a–crRNA
binary
complex
trigger
trans-cleavage
any
by-stander
reporter
single-stranded
(ss)
DNA,
which
converted
amplified
fluorescent
readouts.
ultralocalized
reactor
constructed
reducing
reaction
volume
from
up
picoliter
accommodate
aforementioned
further
enhance
sensitivity
even
render
an
amplification-free
detection.
Moreover,
established
smartphone
App
coupled
with
random
forest
learning
model
based
on
parameters
such
as
area,
intensity,
counting
number
ensure
accuracy
image
recording
processing.
sample-to-result
time
within
80
min.
Importantly,
proposed
able
accurately
detect
ND1
(pork-specific)
IL-2
(duck-specific)
genes
deep
processed
meat-derived
foods
that
usually
had
truncated
results
were
more
robust
practical
than
conventional
real-time
polymerase
chain
after
side-by-side
comparison.
All
all,
expected
used
rapid
food
other
detections
future.
Journal of Food Measurement & Characterization,
Год журнала:
2023,
Номер
18(3), С. 1825 - 1856
Опубликована: Дек. 22, 2023
Abstract
Citrus
is
one
of
the
most
widely
grown
fruits
globally,
because
its
remarkable
organoleptic
features,
nutritional
content
and
bioactive
ingredients.
Microbial
spoilage
other
factors
such
as
physiological
disorder,
mechanical
physical
damage,
fruit
senescence
are
major
postharvest
loss
to
citrus
industry.
The
losses
in
directly
have
negative
impcats
on
economy,
environment
healths
due
production
carbon
emission
gases.
fungal
pathogens
Penicillium
digitatum
,
italicum
Geotrichum
candidum
cause
fruits.
These
produce
different
mycotoxins
citrinin,
patulin,
tremorgenic.
secondary
metabolites
molds;
they
employ
toxic
effects
healths.
acuteness
mytoxin
toxicity
dependings
extent
exposure,
age
status
individual.
mytoxins
related
food
safety
health
concern
including
damage
DNA,
kidney
mutation
RNA/DNA,
growth
impairment
childs
immune
system
etc.
Several
attempts
been
made
extend
shelf-life
by
controlling
decay
which
has
got
limited
success.
In
recent
years,
nanotechnology
emerged
a
new
strategy
for
shelf
life
prevention
biopolymer
based
nano-formulations
functionalized
with
active
compounds
shown
promising
results
maintaining
quality
attributes
vegetables
retarding
moisture
oxidation.
This
review
exclusively
discloses
their
causes.
addition,
use
nanoformulations
agents
developing
technologies
also
discussed
briefly.
nano-formulation
described.The
finding
this
suggest
that
natural
biopolymers
can
be
used
extending
minimizing
an
alternatives
fungicides.
Abstract
In
present
study,
the
effects
of
orange
peel
essential
oil
and
ultrasonic
treatment
on
properties
corn
starch
based
edible
coating
films
including
their
shelf
life
citrus
(Kinnow)
fruits
are
investigated.
The
approach
significantly
improves
quality
characteristics
formulations,
stability,
rheological
behaviour
materials
by
reducing
particle
size,
antimicrobial
activity.
size
reduces
from
2495
nm
(control)
to
298.8
ultrasonication
with
improved
stability
(−39.251
mV)
as
well
tensile
strength
(720
N)
solubility
(32.40%)
CSEOU
film
also
compared
CSEO
CS
films.
formulation
enriched
(CSEOU)
shelf‐life
Kinnow
retarding
weight
loss
(PLW)
microbial
load,
maintaining
higher
acidity
(TA),
total
soluble
solids
(TSS),
antioxidant
treated
samples
indicate
lowest
(24.57%),
pH
(3.89)
TA
(3.999%),
firmness
(494.229
N),
phenolic
(26.17
mg
100
g
−1
),
flavonoid
(34.11
(49.04%),
activity
against
A.
niger
,
E.
coli
S.
aureus
other
samples.
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (CHINESE VERSION),
Год журнала:
2023,
Номер
51(8), С. 100286 - 100286
Опубликована: Май 27, 2023
Metal-organic
frameworks
(MOFs)
are
hybrid
materials
composed
of
both
organic
and
inorganic
elements.
MOF
materials,
because
their
unique
structures
properties,
such
as
controllable
pore
size,
large
surface
area,
excellent
catalytic
activity,
high
density
active
sites,
applied
in
electrochemical
sensors
systems
for
the
sensitive,
quick,
low-cost
detection
multiple
analytes.
MOFs,
with
specific
shape,
adjustable
volume,
will
provide
strong
interaction
compounds
via
abundant
functional
groups,
leading
to
increased
selectivity
determination
a
wide
range
compounds.
In
this
review,
structure,
synthesis
methods,
characterization,
applications
MOFs
recent
years
sensing
material
fabricating
detecting
drugs,
pesticides,
heavy
metals,
food
additives,
other
contaminants
summarized,
compared,
discussed.
Also,
were
used
numerous
antibacterial
application
areas
due
long-term
release
capability,
stretch
ability
when
integrated
and/or
substances
(including
nanostructures,
phytochemicals,
antibiotics,
polymers).
This
review
also
provides
comprehensive
overview
uses
one's
composite
focus
on
different
kinds
nanocomposite
influence
various
uses.
Reviews on Environmental Health,
Год журнала:
2023,
Номер
unknown
Опубликована: Июнь 1, 2023
The
presence
of
trace
elements
such
as
lithium
(Li)
in
water
resources
the
long
term
can
endanger
consumers'
health.
Several
studies
have
been
conducted
on
Li
concentration
sources;
hence,
this
study
attempted
to
retrieve
using
a
systematic
search.
search
was
Web
Sciences,
Embase,
PubMed,
and
Scopus
databases
from
1
January
2010
15
2023.
meta-analyzed
based
type
countries
subgroups
random
effects
model
(REM)
statistical
analysis.
In
addition,
health
risk
assessment
different
age
groups
calculated
target
hazard
quotient
(THQ).
This
included
76
papers
with
157
data
reports
our
meta-analysis.
overall
pooled
5.374
(95
%
CI:
5.261-5.487
μg/L).
groundwater
(40.407
μg/L)
14.53
times
surface
(2.785
highest
content
attributed
Mexico
(2,209.05
μg/L),
Bolivia
(1,444.05
Iraq
(1,350
Argentina
(516.39
At
same
time,
lowest
associated
Morocco
(1.20
Spain
(0.46
India
(0.13
THQ
due
consumers
Iraq,
Mexico,
South
Africa,
Afghanistan,
Bolivia,
Portugal,
Malawi,
Korea,
Nepal,
Argentina,
USA
higher
than
value.
Therefore,
continuous
monitoring
sources
reducing
concentration,
especially
water,
new
treatment
processes
these
are
recommended.
International Journal of Electrochemical Science,
Год журнала:
2023,
Номер
18(10), С. 100290 - 100290
Опубликована: Июль 22, 2023
This
work's
purpose
was
a
design
nanocomposite
of
polyneutral
red
and
reduced
graphene
oxide
paste
electrode
(pNR/rGO-PE)
that
may
be
employed
as
an
accurate
sensitive
electrochemical
sensor
to
measurement
nitrite
level
in
real
food
samples.
The
electro-polymerization
process
for
the
synthesis
pNR/rGO-PE.
Results
from
XRD
SEM
analysis
proved
NR
successfully
electro-polymerized
on
rGO-PE.
incorporation
rGO
nanosheets
into
conducting
polymer
with
synergistic
effects
improved
response
modified
great
selectivity
sensitivity
0.01511
µA/µM
stable
broad
linear
range
0
14000
µM,
according
studies
using
CV
amperometry.
low
detection
limit
17
nM.
accuracy
validation
pNR/rGO-PE
prepared
meat
samples
were
studied.
attained
RSD
values
(3.56%
4.78%)
recovery
(90.00%
96.66%)
amperometry
satisfactory,
confirming
applicability
results
determination
