Spatiotemporal dynamics and selectivity of mRNA translation during mouse pre-implantation development DOI Creative Commons
Hao Ming, Rajan Iyyappan,

Kianoush Kakavand

и другие.

Опубликована: Окт. 28, 2024

Translational regulation is pivotal during preimplantation development. However, how mRNAs are selected for temporal and their dynamic utilization fate this period still unknown. Using a high-resolution ribosome profiling approach, we analyzed the transcriptome, as well monosome- polysome-bound RNAs of mouse oocytes embryos, defining an unprecedented extent spatiotemporal translational landscapes rapid developmental phase. We observed previously unknown mechanisms selectivity, i.e., stage-wise deferral loading monosome-bound to polysome active translation, continuous translation both monosome at same stage, priming monosomes after initial activation. showed that eukaryotic initiation factor Eif1ad3, which exclusively translated in 2-Cell embryo, required biogenesis post embryonic genome Our study thus provides genome-wide datasets analyses dynamics accompanying mammalian germ cell development reveals contribution novel pre-implantation

Язык: Английский

The regulatory landscape of 5′ UTRs in translational control during zebrafish embryogenesis DOI Creative Commons
Madalena M. Reimão-Pinto, Sebastian M. Castillo-Hair, Georg Seelig

и другие.

Developmental Cell, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

The 5' UTRs of mRNAs are critical for translation regulation during development, but their in vivo regulatory features poorly characterized. Here, we report the landscape early zebrafish embryogenesis using a massively parallel reporter assay 18,154 sequences coupled to polysome profiling. We found that UTR suffices confer temporal dynamics initiation and identified 86 motifs enriched with distinct ribosome recruitment capabilities. A quantitative deep learning model, Danio Optimus 5-Prime (DaniO5P), combined role length, site context, upstream AUGs, sequence on recruitment. DaniO5P predicts activities maternal zygotic isoforms indicates modulating length motif grammar contributes dynamics. This study provides first model UTR-based development lays foundation identifying underlying molecular effectors.

Язык: Английский

Процитировано

1
MARTRE family proteins negatively regulate CCR4-NOT activity to protect poly(A) tail length and promote translation of maternal mRNA DOI Creative Commons
Jing Yang, Jiachen Bu, Bowen Liu

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 2, 2025

Язык: Английский

Процитировано

0
Transcriptome and DNA methylation profiling during the NSN to SN transition in mouse oocytes DOI Creative Commons
Hannah Demond, Soumen Khan, Juan Castillo‐Fernandez

и другие.

BMC Molecular and Cell Biology, Год журнала: 2025, Номер 26(1)

Опубликована: Янв. 3, 2025

Abstract Background During the latter stages of their development, mammalian oocytes under dramatic chromatin reconfiguration, transitioning from a non-surrounded nucleolus (NSN) to surrounded (SN) stage, and concomitant transcriptional silencing. Although NSN-SN transition is known be essential for developmental competence oocyte, less about accompanying molecular changes. Here we examine changes in transcriptome DNA methylation during NSN SN mouse oocytes. Results To study dynamics transition, used single-cell (sc)M&T-seq generate scRNA-seq sc-bisulphite-seq (scBS-seq) data GV classified as or by Hoechst staining nuclei. Transcriptome analysis showed lower number detected transcripts compared with well downregulation 576 genes, which were enriched processes related mRNA processing. We classifier that can infer stage datasets. The was successfully tested multiple published datasets models skew NSN: ratios. Analysis scBS-seq increased oocytes, most pronounced regions intermediate levels methylation. Overlap immunoprecipitation sequencing (ChIP-seq) histone modifications H3K36me3, H3K4me3 H3K27me3 gaining are overlapping H3K36me3 H3K27me3, an unusual combination, these marks do not typically coincide. Conclusions characterise develop status bulk RNA-seq data, enabling identification altered genetic knock-outs, quality control identify skewed proportions could otherwise confound differential gene expression analysis. late-methylating associated combination modifications, may high plasticity between reflect heterogeneity on level.

Язык: Английский

Процитировано

0
Cytoplasmic mRNA decay and quality control machineries in eukaryotes DOI
Megan E. Dowdle, Jens Lykke‐Andersen

Nature Reviews Genetics, Год журнала: 2025, Номер unknown

Опубликована: Янв. 27, 2025

Язык: Английский

Процитировано

0
Decoding the Molecular Landscape of Prepubertal Oocyte Maturation: GTPBP4 as a Key Driver of In Vitro Developmental Competence DOI Creative Commons
Jianpeng Qin,

Yaozong Wei,

Ao Ning

и другие.

Cell Proliferation, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

ABSTRACT The intricate mechanisms driving oocyte maturation remain only partially understood, especially within the domains of domestic animal reproduction and translational medicine. In case prepubertal girls, clinical challenge is pronounced, as ovarian tissue cryopreservation‐though promising‐remains an experimental technique necessitating rigorous scientific validation to guarantee developmental potential preserved materials facilitate broader adoption. To address these knowledge gaps, while considering ethical implications, we applied transcriptome translatome sequencing comprehensively profile transcriptional dynamics in adult goats. Our analyses uncovered a sequential transition gene expression regulation, shifting from cytoplasmic processes chromosome segregation during process. Comparative profiling between goat oocytes revealed critical regulatory factors essential for maturation. These include genes involved organelle function ( GTPBP4 TOMM7 ), spindle organisation CKS2 , CCP110 CKAP5 ESCO1 ) CENPE CENPF CENPN SGO2 ). Functional through vitro experiments demonstrated that significantly enhances competence oocytes. This enhancement occurs promote cell cycle progression, mRNA translation. findings provide detailed map molecular events underpinning offer new perspectives on strategies required females. Translating insights humans, this research highlights fertility preservation such cryopreservation transplantation, follicle culture, meiotic artificial ovary technologies. Moreover, identified have significant implications improving reproductive efficiency breeding, bridging basic science.

Язык: Английский

Процитировано

0
SETD2 regulates oocytes in vitro maturation through histone methylation and maternal mRNA degradation in yak DOI

Hui Zhang,

Yangyang Pan, Meng Wang

и другие.

Theriogenology, Год журнала: 2025, Номер 240, С. 117387 - 117387

Опубликована: Март 12, 2025

Язык: Английский

Процитировано

0
ZNHIT3 Regulates Translation to Ensure Cell Lineage Differentiation in Mouse Preimplantation Development DOI Creative Commons
Guanghui Yang,

Qiliang Xin,

Jurrien Dean

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Апрель 3, 2025

Upon fertilization, the mouse zygotic genome is activated and maternal RNAs as well proteins are degraded. Early developmental programs built on encoded by genes that needed to guide early cell fate commitment. The box C/D snoRNA ribonucleoprotein (snoRNP) complex required for rRNA biogenesis, ribosome assembly pre-mRNA splicing essential protein translation. Zinc finger, HIT type 3 (encoded Znhit3) previously identified a component in of snoRNP complex. Using gene-edited mice, it identifies Znhit3 an embryonic gene whose ablation reduces translation prevents embryos development beyond morula stage. absence ZNHIT3 leads decreased abundance which causes defects ribosomes mRNA splicing. Microinjection cRNA partially rescues phenotype confirms during preimplantation development.

Язык: Английский

Процитировано

0
New insights into oocyte cytoplasmic lattice-associated proteins DOI
Carlo Giaccari, Francesco Cecere,

Lucia Argenziano

и другие.

Trends in Genetics, Год журнала: 2024, Номер 40(10), С. 880 - 890

Опубликована: Июль 1, 2024

Язык: Английский

Процитировано

3
HH5 double-carrier embryos fail to progress through early conceptus elongation DOI Creative Commons
Alba Pérez-Gómez, Julieta Gabriela Hamze, Inés Flores-Borobia

и другие.

Journal of Dairy Science, Год журнала: 2024, Номер 107(8), С. 6371 - 6382

Опубликована: Апрель 19, 2024

Massive genotyping in cattle has uncovered several deleterious haplotypes that cause pre-term mortality. Holstein Haplotype 5 (HH5) is a haplotype present the Friesian population involves ablation of Transcription Factor B1 mitochondrial (TFB1M) gene. The developmental stage at which HH5 double-carrier (DC, homozygous) embryos or fetuses die remains unknown and this relevant information to estimate economic losses associated inadvertent cross between carriers. To determine if DC survive maternal recognition pregnancy, embryonic day (E)14 were flushed from superovulated carrier cows inseminated with bull. E14 conceptuses recovered Mendelian rates but they failed achieve early elongation, as evidenced by drastic (>26-fold) reduction proliferation extraembryonic membranes compared non-carrier embryos. assess development earlier stages, TFB1M knockout (KO) -functionally equivalent embryos- generated CRISPR technology cultured blastocyst -Day (D)8- disc -D12-. No significant effect was observed on differentiation lineages relative mtDNA content up D12. In conclusion, are able develop fail undergo conceptus required for pregnancy recognition.

Язык: Английский

Процитировано

2
Advances in PIWI-piRNA function in female reproduction in mammals DOI Creative Commons
Xiaolong Lv, Hongdao Zhang, Ligang Wu

и другие.

Acta Biochimica et Biophysica Sinica, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

PIWI-interacting RNAs (piRNAs), which associate with PIWI clade Argonaute proteins to form piRNA-induced silencing complexes (piRISCs) in germline cells, are responsible for maintaining genomic integrity and reproductive function through transcriptional or post-transcriptional suppression of transposable elements regulation protein-coding genes. Recent discoveries crucial PIWI-piRNA functions oogenesis embryogenesis golden hamsters suggest an indispensable role female fertility that has been obscured the predominant mouse model pathway regulation. In particular, studies piRNA expression dynamics, functional redundancies, compositional variations across mammal species have advanced our understanding male and, especially, reproduction. These findings further support use as a more representative biology mammals. addition discussing these new perspectives, current review also covers emerging directions research, its implications fertility, fundamental mechanisms.

Язык: Английский

Процитировано

1