Large-scale investigation for antimicrobial activity reveals novel defensive species across the healthy skin microbiome DOI Creative Commons

Uyen Thy Nguyen,

Rauf Salamzade, Shelby Sandstrom

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 4, 2024

The human skin microbiome constitutes a dynamic barrier that can impede pathogen invasion by producing antimicrobial natural products. Gene clusters encoding for production of secondary metabolites, biosynthetic gene (BGCs), are enriched in the relative to other ecological settings, position this niche as promising source new product mining. Here, we introduce isolate collection, EPithelial Isolate Collection (EPIC). It includes large phylogenetically diverse set skin-derived bacterial strains from eight body sites. This consisting 980 is larger and more than existing resources, hundreds rare low-abundance species, unique BGCs. Using large-scale co-culture screen assess 8,756 pairwise interactions between skin-associated bacteria potential pathogens, reveal broad antifungal activity members. Integrating 287 whole genomes 268 metagenomes sampling sites demonstrates while distribution BGC types stable across sites, specific cluster families (GCFs), each predicted encode distinct metabolite, substantially vary. Sites dry or rarely moist harbor greatest discovery novel bioactive metabolites. Among our discoveries four three which exert significant broad-spectrum activity. comprehensive collection advances understanding microbiomes capabilities pathogen-fighting mechanisms, opening avenues towards drug engineering.

Язык: Английский

Multi‐omics analysis to evaluate the effects of solar exposure and a broad‐spectrum SPF50+ sunscreen on markers of skin barrier function in a skin ecosystem model DOI
Carine Jacques, Emilien L. Jamin,

Anais Noustens

и другие.

Photochemistry and Photobiology, Год журнала: 2024, Номер unknown

Опубликована: Июль 25, 2024

Abstract Sun exposure induces major skin alterations, but its effects on metabolites and lipids remain largely unknown. Using an original reconstructed human epidermis (RHE) model colonized with microbiota supplemented sebum, we previously showed that a single dose of simulated solar radiation (SSR) significantly impacted the metabolome microbiota. In this article, further analyzed SSR‐induced changes in same RHE model. Among altered (log2‐fold p ≤ 0.05), found several natural moisturizing factors (NMFs): amino acids, lactate, glycerol, urocanic acid, pyrrolidone carboxylic acid derivatives. Analyses stratum corneum also SSR induced lower levels free fatty acids higher ceramides, cholesterols An imbalance NMFs ceramides combined to increase proinflammatory may participate permeability barrier impairment, dehydration inflammatory reaction sun. Our allowed evaluation innovative ultraviolet/blue light (UV/BL) broad‐spectrum sunscreen high sun protection factor (SPF50+). We using prior could part prevent alterations ecosystem

Язык: Английский

Процитировано

0
Large-scale investigation for antimicrobial activity reveals novel defensive species across the healthy skin microbiome DOI Creative Commons

Uyen Thy Nguyen,

Rauf Salamzade, Shelby Sandstrom

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 4, 2024

The human skin microbiome constitutes a dynamic barrier that can impede pathogen invasion by producing antimicrobial natural products. Gene clusters encoding for production of secondary metabolites, biosynthetic gene (BGCs), are enriched in the relative to other ecological settings, position this niche as promising source new product mining. Here, we introduce isolate collection, EPithelial Isolate Collection (EPIC). It includes large phylogenetically diverse set skin-derived bacterial strains from eight body sites. This consisting 980 is larger and more than existing resources, hundreds rare low-abundance species, unique BGCs. Using large-scale co-culture screen assess 8,756 pairwise interactions between skin-associated bacteria potential pathogens, reveal broad antifungal activity members. Integrating 287 whole genomes 268 metagenomes sampling sites demonstrates while distribution BGC types stable across sites, specific cluster families (GCFs), each predicted encode distinct metabolite, substantially vary. Sites dry or rarely moist harbor greatest discovery novel bioactive metabolites. Among our discoveries four three which exert significant broad-spectrum activity. comprehensive collection advances understanding microbiomes capabilities pathogen-fighting mechanisms, opening avenues towards drug engineering.

Язык: Английский

Процитировано

0