bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Сен. 1, 2023
Abstract
Polymicrobial
infection
with
Candida
albicans
and
Staphylococcus
aureus
may
result
in
a
concomitant
increase
virulence
resistance
to
antimicrobial
drugs.
This
enhanced
pathogenicity
phenotype
is
mediated
by
numerous
factors
including
metabolic
processes
direct
interaction
of
S.
C.
hyphae.
The
overall
structure
biofilms
known
contribute
their
recalcitrance
treatment,
however
the
dynamics
between
species
how
it
contributes
poorly
understood.
To
address
this,
novel
time-lapse
mesoscopic
optical
imaging
method
was
developed
enable
formation
albicans/S.
whole
dual-species
be
followed.
It
found
that
yeast-form
or
hyphal-form
biofilm
founder-population
profoundly
affects
as
matures.
Different
sub-populations
arise
within
each
different
morphotypes,
resulting
distinct
sub-regions.
These
data
reveal
cell
morphology
pivotal
development
global
architecture
emergence
colony
macrostructures
temporally
influence
synergy
infection.
Trends in Microbiology,
Год журнала:
2024,
Номер
32(11), С. 1106 - 1118
Опубликована: Май 9, 2024
The
human
gastrointestinal
microbiome
encompasses
bacteria,
fungi,
and
viruses
forming
complex
bionetworks
which,
for
organismal
health,
must
be
in
a
state
of
homeostasis.
An
important
homeostatic
mechanism
derives
from
microbial
competition,
which
maintains
the
relative
abundance
species
healthy
balance.
Microbes
compete
nutrients
secrete
metabolites
that
inhibit
other
microbes.
Short-chain
fatty
acids
(SCFAs)
are
one
such
class
made
by
gut
bacteria
to
very
high
levels.
SCFAs
metabolised
microbes
host
cells
have
multiple
roles
regulating
cell
physiology.
Here,
we
review
mechanisms
regulate
fungal
commensal
Candida
albicans.
We
discuss
SCFA's
ability
growth,
limit
invasive
behaviours
modulate
surface
antigens
recognised
immune
cells.
underlying
these
roles:
regulation
gene
expression,
metabolism,
signalling
SCFA-driven
post-translational
protein
modifications
acylation,
contribute
changes
acylome
dynamics
C.
albicans
with
potentially
large
consequences
Given
mycobiome
is
reservoir
systemic
disease
has
also
been
implicated
inflammatory
bowel
disease,
understanding
bacterial
metabolites,
as
SCFAs,
control
might
provide
therapeutic
avenues.
Infection and Immunity,
Год журнала:
2024,
Номер
92(9)
Опубликована: Апрель 22, 2024
ABSTRACT
The
intestinal
microbiome
harbors
fungi
that
pose
a
significant
risk
to
human
health
as
opportunistic
pathogens
and
drivers
of
inflammation.
Inflammatory
autoimmune
diseases
are
associated
with
dysbiotic
fungal
communities
the
expansion
potentially
pathogenic
fungi.
gut
is
also
main
reservoir
for
disseminated
infections.
Immune
interactions
critical
preventing
commensal
from
becoming
pathogenic.
Significant
strides
have
been
made
in
defining
innate
adaptive
immune
pathways
regulate
fungi,
these
discoveries
coincided
advancements
our
understanding
molecular
effectors
involved
both
colonization
pathogenesis
within
gut.
In
this
review,
we
will
discuss
important
regulating
focus
on
how
specific
cell
types
interact
limit
their
or
potential.
This
include
target
orchestrate
antifungal
responses,
addition
secreted
effectors,
such
mucus
antimicrobial
peptides,
inhibit
These
be
framed
around
current
niche.
Finally,
highlight
unexplored
mechanisms
by
which
system
regulates
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Июль 9, 2024
Candida
albicans
and
Staphylococcus
aureus
are
two
commonly
associated
pathogens
that
cause
nosocomial
infections
with
high
morbidity
mortality.
Our
prior
current
work
using
a
murine
model
of
polymicrobial
intra-abdominal
infection
(IAI)
demonstrates
synergistic
lethality
is
driven
by
Candida-induced
upregulation
functional
S.
α-toxin
leading
to
sepsis
organ
damage.
In
order
determine
the
candidal
effector(s)
mediating
enhanced
virulence,
an
unbiased
screen
C.
transcription
factor
mutants
was
undertaken
revealing
zcf13Δ/Δ
fails
drive
augmented
or
lethal
synergism
during
co-infection.
A
combination
transcriptional
phenotypic
profiling
approaches
shows
ZCF13
regulates
genes
involved
in
pentose
metabolism,
including
RBK1
HGT7
contribute
fungal
ribose
catabolism
uptake,
respectively.
Subsequent
experiments
reveal
inhibits
staphylococcal
agr
quorum
sensing
system
concomitantly
represses
toxicity.
Unlike
wild-type
albicans,
did
not
effectively
utilize
co-culture
co-infection
exogenous
accumulation
repression.
Forced
expression
mutant
fully
restores
pathogenicity
Collectively,
our
results
detail
interwoven
complexities
cross-kingdom
interactions
highlight
how
intermicrobial
metabolism
impacts
disease
pathogenesis
devastating
consequences
for
host.
International Endodontic Journal,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 17, 2025
Abstract
Aim
Previous
studies
have
shown
the
important
relationships
between
Enterococcus
faecalis
and
Candida
albicans
in
post‐treatment
endodontic
disease
(PTED).
However,
fungal–bacterial
interactions
their
possible
functional
routes
are
less
understood.
In
this
study,
we
investigated
effect
of
extracellular
vesicles
(EVs)
derived
from
C.
on
E.
growth
pathogenicity.
Methodology
EVs
were
isolated
a
yeast
nitrogen
base
(YNB)
medium,
morphology,
size
distribution,
protein
concentration
observed
identified.
The
effects
planktonic
evaluated
using
curves
colony‐forming
unit
counts,
whereas
biofilms
determined
scanning
electron
confocal
laser
microscopes.
ability
to
resist
detrimental
environment,
infect
dentinal
tubules,
biofilm
formation
gutta
percha
was
examined.
Additionally,
cell
invasion
cytotoxicity
assessed.
Statistical
analysis
performed
one‐way
variance,
p
‐values
<.05
considered
significantly
different.
Results
nanoparticles
with
bilayer
membranes
peak
sizes
111.9
230
nm.
exhibited
complex
its
biofilms;
5
μg/mL
showed
inhibitory
0.156
facilitated
growth.
consistent
virulence.
Notably,
reduced
damage
RAW264.7
cells
caused
by
,
as
well
macrophages
intracellular
survival
after
macrophage
phagocytosis,
had
completely
opposite
effects.
Conclusion
dual
virulence
vitro
suggesting
involved
communication.
Moreover,
may
suggest
new
agent
for
control
.
Communications Biology,
Год журнала:
2025,
Номер
8(1)
Опубликована: Фев. 3, 2025
Historical
processes
in
community
assembly,
such
as
species
arrival
order,
influence
interactions,
causing
priority
effects.
Candida
albicans
and
Pseudomonas
aeruginosa
often
co-occur
biofilm-based
infections
of
the
skin,
lungs,
medical
devices.
Their
predominantly
antagonistic
relationship
involves
complex
physical
chemical
interactions.
However,
presence
implications
effects
among
these
microorganisms
remain
largely
unexplored.
Here,
we
investigate
impact
effect
dual-species
biofilms
using
clinical
isolates.
By
varying
inoculation
observe
significant
changes
biofilm
composition,
structure,
virulence,
antimicrobial
susceptibility.
The
first
colonizer
has
an
advantage
for
surface
colonization.
Consecutive
colonization
increases
virulence
negates
C.
albicans'
protective
on
P.
PAET1
against
meropenem
treatment.
Finally,
propose
N-acetylcysteine
adjuvant
treating
interkingdom
infections,
working
independently
Polymicrobial
demonstrated
that
effects,
influenced
by
species'
affect
Trends in Microbiology,
Год журнала:
2023,
Номер
31(12), С. 1276 - 1286
Опубликована: Авг. 30, 2023
In
silico
and
experimental
approaches
have
allowed
an
ever-growing
understanding
of
the
interactions
within
microbiota.
For
instance,
recently
acquired
data
increased
knowledge
mechanisms
that
support,
in
gut
vaginal
microbiota,
resistance
to
colonization
by
Candida
albicans,
opportunistic
fungal
pathogen
whose
overgrowth
can
initiate
severe
infections
immunocompromised
patients.
Here,
we
review
how
bacteria
from
microbiota
interact
with
C.
albicans.
We
show
recent
OMICs-based
pipelines,
using
metagenomics
and/or
metabolomics,
identified
bacterial
species
metabolites
modulating
albicans
growth.
finally
discuss
combined
use
cutting-edge
could
provide
new
means
control
prevent
its
consequences.
