The Role of G-Protein-Coupled Receptor Kinase 4 in Modulating Mitophagy and Oxidative Stress in Cerebral Ischemia–Reperfusion Injury
NeuroMolecular Medicine,
Год журнала:
2025,
Номер
27(1)
Опубликована: Март 7, 2025
Язык: Английский
Oroxylin A reverses SHP-2 oxidative inactivation in GPVI signaling to suppress platelet activation and thrombus formation
Thrombosis Journal,
Год журнала:
2025,
Номер
23(1)
Опубликована: Март 18, 2025
Arterial
thrombotic
events
are
the
leading
causes
of
death
worldwide,
and
therapeutic
effects
current
antiplatelet
drugs
not
fully
satisfactory.
Oroxylin
A
(OroA),
a
flavone
compound
extracted
from
Scutellaria
radix,
possesses
cardioprotective
many
other
pharmacological
effects.
While
platelets
play
crucial
role
in
development
myocardial
infarction,
direct
OroA
on
platelet
activation
thrombosis
have
yet
to
be
investigated.
FeCl₃-induced
arteriole
whole-blood
perfusion
were
used
assess
inhibitory
effect
thrombus
formation.
ischemia
model
was
employed
evaluate
protective
injury.
Multiple
function
studies
including
aggregation,
spreading,
clot
retraction
performed.
Network
pharmacology,
flow
cytometry,
enzyme-linked
immunosorbent
assay,
co-immunoprecipitation
western
blot
utilized
explore
mechanism
activation.
inhibited
formation
with
less
bleeding
risk
compared
aspirin.
protected
against
injury
by
suppressing
microvascular
infiltration.
suppressed
different
agonist-induced
concentration-dependent
manner,
showing
greater
activity
collagen-induced
aggregation
ADP
or
thrombin-induced
aggregation.
decreased
granule
release,
integrin
αIIbβ3
activation,
spreading
retraction.
As
flavone,
boosted
superoxide
dismutase
(SOD)
glutathione
(GSH)
activities
malondialdehyde
(MDA),
oxidized
(GSSG)
ROS
levels
during
oxidative
stress.
binds
SHP-2
prevents
its
inactivation,
tyrosine
dephosphorylation
Src,
Syk
PLCγ2,
as
well
reduction
Ca2+
influx
PKC
phosphorylation
GPVI
signaling.
inhibits
via
reversing
inactivation
thereby
attenuating
With
minor
no
obvious
toxicity,
holds
promising
potential
an
antithrombotic
drug.
Язык: Английский
DRP1, fission and apoptosis
Cell Death Discovery,
Год журнала:
2025,
Номер
11(1)
Опубликована: Апрель 7, 2025
Язык: Английский
The foam cell-derived exosomes exacerbate ischemic white matter injury via transmitting metabolic defects to microglia
Cell Metabolism,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 1, 2025
Язык: Английский
Cell life-or-death events in osteoporosis: All roads lead to mitochondrial dynamics
Pharmacological Research,
Год журнала:
2024,
Номер
208, С. 107383 - 107383
Опубликована: Авг. 28, 2024
Mitochondria
exhibit
heterogeneous
shapes
and
networks
within
among
cell
types
tissues,
also
in
normal
or
osteoporotic
bone
tissues
with
complex
types.
This
dynamic
characteristic
is
determined
by
the
high
plasticity
provided
mitochondrial
dynamics
stemmed
from
responding
to
survival
functional
requirements
of
various
cells
a
specific
microenvironments.
In
contrast,
dysfunction,
induced
dysregulation
dynamics,
may
act
as
trigger
death
signals,
including
common
apoptosis
other
forms
programmed
(PCD).
These
PCD
processes
consisting
tightly
structured
cascade
gene
expression
events,
can
further
influence
remodeling
facilitating
cells.
Mitochondrial
therefore,
drive
stand
at
crossroads
life
integrating
external
signals
altering
metabolism,
shape,
signal-response
properties
mitochondria.
implies
that
targeting
displays
significant
potential
treatment
osteoporosis.
Considerable
effort
has
been
made
osteoporosis
emphasize
parallel
roles
mitochondria
regulating
energy
calcium
signal
transduction,
oxidative
stress,
inflammation,
death.
However,
emerging
field
dynamics-related
not
well
understood.
Herein,
bridge
gap,
we
outline
latest
knowledge
on
during
Язык: Английский
Synergistic and Antagonistic Activity of Selected Dietary Phytochemicals against Oxidative Stress-Induced Injury in Cardiac H9c2 Cells via the Nrf2 Signaling Pathway
Foods,
Год журнала:
2024,
Номер
13(15), С. 2440 - 2440
Опубликована: Авг. 2, 2024
The
antioxidant
activities
of
lycopene
(LY),
lutein
(LU),
chlorogenic
acid
(CA),
and
delphinidin
(DP)
were
tested
in
vitro
on
H9c2
cell-based
models.
Some
indicators,
such
as
the
generation
reactive
oxygen
(ROS),
quantification
cell
activity
(CAA),
expressions
SOD,
GSH-Px,
CAT,
calculated
to
examine
their
interactions.
From
our
results,
phytochemical
mixtures
(M1:
CA-LU:
F3/10,
M2:
DP-CA:
F7/10,
M3:
DP-LY:
F5/10)
displayed
strong
synergistic
effects
based
ROS
CAA.
However,
great
antagonistic
bioactivities
seen
combinations
LY-LU:
F5/10
(M4),
F9/10
(M5),
F7/10
(M6).
Western
blotting
analysis
indicated
that
possible
mechanism
underlying
interactions
among
was
enhance
accumulation
Nrf2
nucleus
expression
its
downstream
enzymes,
HO-1
GCLC.
(M1–M3
groups)
showed
significant
protection
against
loss
mitochondrial
membrane
potential
than
individual
groups
avoid
excessive
production.
M4–M6
exerted
protective
compared
with
groups.
In
addition,
absorption
improved
more
because
presence
M1
M3
groups,
respectively.
significantly
reduced
cellular
uptake
M6
group.
It
appeared
may
contribute
restoration
redox
homeostasis
lead
an
improvement
diet
quality
collocation.
Язык: Английский