Synergistic and Antagonistic Activity of Selected Dietary Phytochemicals against Oxidative Stress-Induced Injury in Cardiac H9c2 Cells via the Nrf2 Signaling Pathway DOI Creative Commons
Jingwen Yu, Xiangwei Xiao,

Baiying Chen

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(15), P. 2440 - 2440

Published: Aug. 2, 2024

The antioxidant activities of lycopene (LY), lutein (LU), chlorogenic acid (CA), and delphinidin (DP) were tested in vitro on H9c2 cell-based models. Some indicators, such as the generation reactive oxygen (ROS), quantification cell activity (CAA), expressions SOD, GSH-Px, CAT, calculated to examine their interactions. From our results, phytochemical mixtures (M1: CA-LU: F3/10, M2: DP-CA: F7/10, M3: DP-LY: F5/10) displayed strong synergistic effects based ROS CAA. However, great antagonistic bioactivities seen combinations LY-LU: F5/10 (M4), F9/10 (M5), F7/10 (M6). Western blotting analysis indicated that possible mechanism underlying interactions among was enhance accumulation Nrf2 nucleus expression its downstream enzymes, HO-1 GCLC. (M1–M3 groups) showed significant protection against loss mitochondrial membrane potential than individual groups avoid excessive production. M4–M6 exerted protective compared with groups. In addition, absorption improved more because presence M1 M3 groups, respectively. significantly reduced cellular uptake M6 group. It appeared may contribute restoration redox homeostasis lead an improvement diet quality collocation.

Language: Английский

The Role of G-Protein-Coupled Receptor Kinase 4 in Modulating Mitophagy and Oxidative Stress in Cerebral Ischemia–Reperfusion Injury DOI
Jian Wang,

Diheng Gu,

Ke Jin

et al.

NeuroMolecular Medicine, Journal Year: 2025, Volume and Issue: 27(1)

Published: March 7, 2025

Language: Английский

Citations

0
Oroxylin A reverses SHP-2 oxidative inactivation in GPVI signaling to suppress platelet activation and thrombus formation DOI Creative Commons
Yufei Chen, Yuan Lin, Jin Tae Hong

et al.

Thrombosis Journal, Journal Year: 2025, Volume and Issue: 23(1)

Published: March 18, 2025

Arterial thrombotic events are the leading causes of death worldwide, and therapeutic effects current antiplatelet drugs not fully satisfactory. Oroxylin A (OroA), a flavone compound extracted from Scutellaria radix, possesses cardioprotective many other pharmacological effects. While platelets play crucial role in development myocardial infarction, direct OroA on platelet activation thrombosis have yet to be investigated. FeCl₃-induced arteriole whole-blood perfusion were used assess inhibitory effect thrombus formation. ischemia model was employed evaluate protective injury. Multiple function studies including aggregation, spreading, clot retraction performed. Network pharmacology, flow cytometry, enzyme-linked immunosorbent assay, co-immunoprecipitation western blot utilized explore mechanism activation. inhibited formation with less bleeding risk compared aspirin. protected against injury by suppressing microvascular infiltration. suppressed different agonist-induced concentration-dependent manner, showing greater activity collagen-induced aggregation ADP or thrombin-induced aggregation. decreased granule release, integrin αIIbβ3 activation, spreading retraction. As flavone, boosted superoxide dismutase (SOD) glutathione (GSH) activities malondialdehyde (MDA), oxidized (GSSG) ROS levels during oxidative stress. binds SHP-2 prevents its inactivation, tyrosine dephosphorylation Src, Syk PLCγ2, as well reduction Ca2+ influx PKC phosphorylation GPVI signaling. inhibits via reversing inactivation thereby attenuating With minor no obvious toxicity, holds promising potential an antithrombotic drug.

Language: Английский

Citations

0
DRP1, fission and apoptosis DOI Creative Commons
Nan Wang,

X.J. Wang,

Beiwu Lan

et al.

Cell Death Discovery, Journal Year: 2025, Volume and Issue: 11(1)

Published: April 7, 2025

Language: Английский

Citations

0
The foam cell-derived exosomes exacerbate ischemic white matter injury via transmitting metabolic defects to microglia DOI
Hang Zhang, Luo‐Qi Zhou, Sheng Yang

et al.

Cell Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Cell life-or-death events in osteoporosis: All roads lead to mitochondrial dynamics DOI Creative Commons
Zhichao Li,

Songlin Liang,

Liqing Ke

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 208, P. 107383 - 107383

Published: Aug. 28, 2024

Mitochondria exhibit heterogeneous shapes and networks within among cell types tissues, also in normal or osteoporotic bone tissues with complex types. This dynamic characteristic is determined by the high plasticity provided mitochondrial dynamics stemmed from responding to survival functional requirements of various cells a specific microenvironments. In contrast, dysfunction, induced dysregulation dynamics, may act as trigger death signals, including common apoptosis other forms programmed (PCD). These PCD processes consisting tightly structured cascade gene expression events, can further influence remodeling facilitating cells. Mitochondrial therefore, drive stand at crossroads life integrating external signals altering metabolism, shape, signal-response properties mitochondria. implies that targeting displays significant potential treatment osteoporosis. Considerable effort has been made osteoporosis emphasize parallel roles mitochondria regulating energy calcium signal transduction, oxidative stress, inflammation, death. However, emerging field dynamics-related not well understood. Herein, bridge gap, we outline latest knowledge on during

Language: Английский

Citations

2
Synergistic and Antagonistic Activity of Selected Dietary Phytochemicals against Oxidative Stress-Induced Injury in Cardiac H9c2 Cells via the Nrf2 Signaling Pathway DOI Creative Commons
Jingwen Yu, Xiangwei Xiao,

Baiying Chen

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(15), P. 2440 - 2440

Published: Aug. 2, 2024

The antioxidant activities of lycopene (LY), lutein (LU), chlorogenic acid (CA), and delphinidin (DP) were tested in vitro on H9c2 cell-based models. Some indicators, such as the generation reactive oxygen (ROS), quantification cell activity (CAA), expressions SOD, GSH-Px, CAT, calculated to examine their interactions. From our results, phytochemical mixtures (M1: CA-LU: F3/10, M2: DP-CA: F7/10, M3: DP-LY: F5/10) displayed strong synergistic effects based ROS CAA. However, great antagonistic bioactivities seen combinations LY-LU: F5/10 (M4), F9/10 (M5), F7/10 (M6). Western blotting analysis indicated that possible mechanism underlying interactions among was enhance accumulation Nrf2 nucleus expression its downstream enzymes, HO-1 GCLC. (M1–M3 groups) showed significant protection against loss mitochondrial membrane potential than individual groups avoid excessive production. M4–M6 exerted protective compared with groups. In addition, absorption improved more because presence M1 M3 groups, respectively. significantly reduced cellular uptake M6 group. It appeared may contribute restoration redox homeostasis lead an improvement diet quality collocation.

Language: Английский

Citations

1