Optimization of Betulinic and Ursolic Acids and Phenolics Extraction from Endemic Rosa pisiformis Using Box-Behnken Design in Relation to Cytotoxic Activities DOI
Hatice Demiray,

Mesut Emir,

Onur Özel

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Abstract Despite its important pharmacological bioactivities, betulinic acid is still primarily obtained through extraction from heartwood and bark or synthesized synthetically, with less than 3% efficiency. Our endemic rose species, Rosa pisiformis (Christ.) D. Sosn., which a new alternative source of traditionally valued for medicinal properties, was collected natural distribution in Gümüşhane province. The plant’s organs, such as root, stem, leaf fruit were air-dried pulverized. compounds separately extracted using three different solvents (ethanol, dichloromethane hexane) an optimized Box-Behnken method. amounts quercetin, rutin, catechin, betulinic, ursolic oleanolic acids roots, stems, leaves, fruits determined HPLC-DAD techniques standard substances. Optimisation data revealed 65% solvent ratio five-times maceration 75 ml solvents. highest catechin found the leaves (DCM) 15.61 µg/ml. Stems rich rutin (28.96 µg/ml) quercetin (39.90 µg/ml). Betulinic content first time stems (hexane, 11.84 roots (9.32 Their cytotoxic activities against prostate lung carcinoma cells evaluated ABTS-assay, revealing that exhibited activity, followed by roots.

Язык: Английский

T-2 Toxin Nephrotoxicity: Toxic Effects, Mechanisms, Mitigations, and Future Perspectives DOI

Youshuang Wang,

Pengli Liu,

Jiayan Fan

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 27, 2025

T-2 toxin is a highly toxic fungal that threatens humans and animals' health. As major detoxifying metabolic organ, the kidney also target of toxin. This article reviews nephrotoxicity research progress, covering renal structure function damage, mechanisms, detoxification methods to future directions. damages structure, causing dysfunction. The mechanism involves multiple factors including oxidative stress, endoplasmic reticulum mitophagy, inflammatory responses, apoptosis, which are intertwined interdependent. Current strategies mainly involve reducing in feedstuff using antioxidant substances, but both have limits. Future should focus on cells sensitivity toxin, exploring key molecules toxin's nephrotoxicity, injury's impact other organs, better methods. review aims guide underpin toxin-induced prevention treatment.

Язык: Английский

Процитировано

1
Optimization of betulinic and ursolic acids and phenolics extraction from endemic Rosa pisiformis using Box-Behnken design in relation to cytotoxic activities DOI Creative Commons
Hatice Demiray,

Mesut Emir Dibek,

Onur Özel

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 7, 2025

Despite its important pharmacological bioactivities, betulinic acid is still primarily obtained through extraction from heartwood and bark or synthesized synthetically, with less than 3% efficiency. Our endemic rose species, Rosa pisiformis (Christ.) D. Sosn., which a new alternative source of traditionally valued for medicinal properties, was collected natural distribution in Gümüşhane province. The plant's organs, such as root, stem, leaf fruit were air-dried pulverized. compounds separately extracted using three different solvents (ethanol, dichloromethane hexane) an optimized Box-Behnken method. amounts quercetin, rutin, catechin, betulinic, ursolic oleanolic acids roots, stems, leaves, fruits determined HPLC–DAD techniques standard substances. Optimisation data revealed 65% solvent ratio five-times maceration 75 ml solvents. highest catechin found the leaves (DCM) 15.61 µg/ml. Stems rich rutin (28.96 µg/ml) quercetin (39.90 µg/ml). Betulinic content first time stems (hexane, 11.84 roots (9.32 Their cytotoxic activities against prostate lung carcinoma cells evaluated ABTS-assay, revealing that exhibited activity, followed by roots.

Язык: Английский

Процитировано

0
A preliminary study of T-2 toxin that cause liver injury in rats via the NF-kB and NLRP3-mediated pyroptosis pathway DOI

Shicong Zheng,

Haonan Li,

Hexuan Dong

и другие.

Toxicon, Год журнала: 2024, Номер 249, С. 108060 - 108060

Опубликована: Авг. 6, 2024

Язык: Английский

Процитировано

0
Optimization of Betulinic and Ursolic Acids and Phenolics Extraction from Endemic Rosa pisiformis Using Box-Behnken Design in Relation to Cytotoxic Activities DOI
Hatice Demiray,

Mesut Emir,

Onur Özel

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Abstract Despite its important pharmacological bioactivities, betulinic acid is still primarily obtained through extraction from heartwood and bark or synthesized synthetically, with less than 3% efficiency. Our endemic rose species, Rosa pisiformis (Christ.) D. Sosn., which a new alternative source of traditionally valued for medicinal properties, was collected natural distribution in Gümüşhane province. The plant’s organs, such as root, stem, leaf fruit were air-dried pulverized. compounds separately extracted using three different solvents (ethanol, dichloromethane hexane) an optimized Box-Behnken method. amounts quercetin, rutin, catechin, betulinic, ursolic oleanolic acids roots, stems, leaves, fruits determined HPLC-DAD techniques standard substances. Optimisation data revealed 65% solvent ratio five-times maceration 75 ml solvents. highest catechin found the leaves (DCM) 15.61 µg/ml. Stems rich rutin (28.96 µg/ml) quercetin (39.90 µg/ml). Betulinic content first time stems (hexane, 11.84 roots (9.32 Their cytotoxic activities against prostate lung carcinoma cells evaluated ABTS-assay, revealing that exhibited activity, followed by roots.

Язык: Английский

Процитировано

0