Exploring the potential role of four Rhizophagus irregularis nuclear effectors: opportunities and technical limitations DOI Creative Commons
Maria Chacon,

Sofía Hernández Luelmo,

Viktor Devlieghere

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Апрель 24, 2024

Arbuscular mycorrhizal fungi (AMF) are obligate symbionts that interact with the roots of most land plants. The genome AMF model species Rhizophagus irregularis contains hundreds predicted small effector proteins secreted extracellularly but also into plant cells to suppress immunity and modify physiology establish a niche for growth. Here, we investigated role four nuclear-localized putative effectors, i.e. , GLOIN707, GLOIN781, GLOIN261, RiSP749, in mycorrhization We initially intended execute functional studies Solanum lycopersicum host economic interest not previously used biology, extended our Medicago truncatula as well non-host Arabidopsis thaliana because technical advantages working these models. Furthermore, three implementation reverse genetic tools, yeast two-hybrid screening whole-genome transcriptome analysis revealed potential nuclear targets downstream triggered transcriptional responses. identified validated protein interactors participating host. S. demonstrated by transcriptomics effectors possible involvement different molecular processes, regulation DNA replication, methylglyoxal detoxification, RNA splicing. conclude R. may act on pathways modulate symbiosis discuss pros cons tools used.

Язык: Английский

Unconventional secretion of Magnaporthe oryzae effectors in rice cells is regulated by tRNA modification and codon usage control DOI
Gang Li, Nawaraj Dulal,

Ziwen Gong

и другие.

Nature Microbiology, Год журнала: 2023, Номер 8(9), С. 1706 - 1716

Опубликована: Авг. 10, 2023

Язык: Английский

Процитировано

23

Effectors and environment modulating rice blast disease: from understanding to effective control DOI
Yanjun Kou, Huanbin Shi, Jiehua Qiu

и другие.

Trends in Microbiology, Год журнала: 2024, Номер 32(10), С. 1007 - 1020

Опубликована: Апрель 5, 2024

Язык: Английский

Процитировано

6

Characterization of Arbuscular Mycorrhizal Effector Proteins DOI Open Access
Maria Chacon, Judith Van Dingenen, Sofie Goormachtig

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(11), С. 9125 - 9125

Опубликована: Май 23, 2023

Plants are colonized by various fungi with both pathogenic and beneficial lifestyles. One type of colonization strategy is through the secretion effector proteins that alter plant's physiology to accommodate fungus. The oldest plant symbionts, arbuscular mycorrhizal (AMF), may exploit effectors their benefit. Genome analysis coupled transcriptomic studies in different AMFs has intensified research on function, evolution, diversification AMF. However, current 338 predicted from AM fungus

Язык: Английский

Процитировано

14

Filamentous pathogen effectors enter plant cells via endocytosis DOI Creative Commons
Haixia Wang, Ely Oliveira‐Garcia, Petra C. Boevink

и другие.

Trends in Plant Science, Год журнала: 2023, Номер 28(11), С. 1214 - 1217

Опубликована: Авг. 14, 2023

Recent findings demonstrate that cytoplasmic effectors from fungal and oomycete pathogens enter plant cells via clathrin-mediated endocytosis (CME). This raises several questions: Does effector secretion pathway facilitate host uptake? How is CME triggered in cells? are the released endosomal compartments to reach diverse subcellular destinations?

Язык: Английский

Процитировано

12

The Dynamic Interaction between Oil Palm and Phytophthora palmivora in Bud Rot Disease: Insights from Transcriptomic Analysis and Network Modelling DOI Creative Commons
Mariandrea García-Gaona, David Botero-Rozo,

Leonardo Araque

и другие.

Journal of Fungi, Год журнала: 2024, Номер 10(3), С. 164 - 164

Опубликована: Фев. 20, 2024

Bud Rot, caused by Phytophthora palmivora, is considered one of the main diseases affecting African oil palm (Elaeis guineensis). In this study, we investigated in vitro molecular dynamics pathogen–host interaction analyzing gene expression profiles from genotypes that were either susceptible or resistant to disease. We observed distinct interactions P. palmivora with and palms through co-expression network analysis. When interacting genotypes, exhibited upregulation carbohydrate sulfate transport genes. These genes demonstrated apoplastic cytoplasmic effectors, including cell wall degrading enzymes, elicitins, RxLR motif effectors. The pathogen manipulated materials, exacerbating response compromising phenylpropanoid pathway, ultimately leading susceptibility. contrast, materials control over their putative Heat Shock Proteins (HSP) maintained homeostasis between primary metabolism biotic defense. Co-expressed related flavonoids, WRKY transcripts, lectin-type receptors, LRR receptors may play important roles control. Overall, study provides new knowledge mechanisms underlying E. guineensis which can contribute controlling Rot gives insights into hosts.

Язык: Английский

Процитировано

4

The secreted protein FonCHRD is essential for vegetative growth, asexual reproduction, and pathogenicity in watermelon Fusarium wilt fungus DOI Creative Commons

Jiajun Lou,

Jiajing Wang, Shaoqun Zeng

и другие.

Crop Health, Год журнала: 2024, Номер 2(1)

Опубликована: Окт. 25, 2024

Abstract Fungal pathogens often secrete numerous effectors to interfere with and/or suppress plant immunity promote their infection. Watermelon Fusarium wilt, caused by the soil-borne fungus oxysporum f. sp. niveum ( Fon ), is one of devastating diseases that severely affect watermelon industry. Here, we report function a candidate effector protein, FonCHRD, in . FonCHRD harbors chordin (CHRD) domain unknown and has signal peptide secretion activity. shows relatively high expression level marcoconidia inducible root tissues. Phenotypic analysis targeted deletion mutant revealed plays roles vegetative growth, asexual reproduction, conidial morphology , while it not involved spore germination as well cell wall, oxidative salt stress responses. Deletion impaired ability colonize spread within host plants, significantly reducing its virulence on watermelon. distributed across multiple compartments cells but can target apoplast space plants. inhibits INF1- Bcl2-associated X protein-triggered death defense gene transiently expressed Nicotiana benthamiana leaves. These findings suggest essential for pathogenicity modulating invasive growth spreading abilities suppressing immune

Язык: Английский

Процитировано

2

Paths of Least Resistance: Unconventional Effector Secretion by Fungal and Oomycete Plant Pathogens DOI Creative Commons
Nawaraj Dulal, Richard A. Wilson

Molecular Plant-Microbe Interactions, Год журнала: 2024, Номер 37(9), С. 653 - 661

Опубликована: Июль 1, 2024

Effector secretion by different routes mediates the molecular interplay between host plant and pathogen, but mechanistic details in eukaryotes are sparse. This may limit discovery of new effectors that could be utilized for improving disease resistance. In fungi oomycetes, apoplastic secreted via conventional endoplasmic reticulum (ER)-Golgi pathway, while cytoplasmic packaged into vesicles bypass Golgi an unconventional protein (UPS) pathway.

Язык: Английский

Процитировано

1

Transformation‐based gene silencing and functional characterization of an ISC effector reveal how a powdery mildew fungus disturbs salicylic acid biosynthesis and immune response in the plant DOI Creative Commons

Jinyao Yin,

Xiao Li,

Longying Dong

и другие.

Molecular Plant Pathology, Год журнала: 2024, Номер 25(11)

Опубликована: Ноя. 1, 2024

Abstract Obligate biotrophic powdery mildew fungi infect a wide range of economically important plants. These often deliver effector proteins into the host tissues to suppress plant immunity and sustain infection. The phytohormone salicylic acid (SA) is one most signals that activate against pathogens. However, how effectors interact with SA signalling poorly understood. Isochorismatase (ISC) from two other filamentous pathogens have been found inhibit biosynthesis by hydrolysing isochorismate, main precursor in cytosol. Here, we identified an ISC effector, named EqIsc1, rubber tree fungus Erysiphe quercicola . In enzyme assays, EqIsc1 displayed activity transferring isochorismate 2,3‐dihydro‐2,3‐dihydroxybenzoate vitro transgenic Nicotiana benthamiana EqIsc1‐expressing Arabidopsis thaliana , SA‐mediated immune response were significantly inhibited. addition, developed electroporation‐mediated transformation method for genetic manipulation E. Inoculation leaves ‐silenced strain induced immunity. We also detected translocation cytosol during interaction between its host. Taken together, our results suggest functions as hydrolyse cytosol, altering response.

Язык: Английский

Процитировано

1

Fusarium sacchari Effector FsMEP1 Contributes to Virulence by Disturbing Localization of Thiamine Thiazole Synthase ScTHI2 from Sugarcane DOI Open Access
Lulu Wang, Deng Wu,

Tianshu Hong

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(22), С. 12075 - 12075

Опубликована: Ноя. 10, 2024

is a significant pathogenic fungus that causes sugarcane Pokkah Boeng. Proteins secreted by fungi can be delivered into hosts to suppress plant immunity and establish infection. However, there still much discovered regarding

Язык: Английский

Процитировано

0

Exploring the potential role of four Rhizophagus irregularis nuclear effectors: opportunities and technical limitations DOI Creative Commons
Maria Chacon,

Sofía Hernández Luelmo,

Viktor Devlieghere

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Апрель 24, 2024

Arbuscular mycorrhizal fungi (AMF) are obligate symbionts that interact with the roots of most land plants. The genome AMF model species Rhizophagus irregularis contains hundreds predicted small effector proteins secreted extracellularly but also into plant cells to suppress immunity and modify physiology establish a niche for growth. Here, we investigated role four nuclear-localized putative effectors, i.e. , GLOIN707, GLOIN781, GLOIN261, RiSP749, in mycorrhization We initially intended execute functional studies Solanum lycopersicum host economic interest not previously used biology, extended our Medicago truncatula as well non-host Arabidopsis thaliana because technical advantages working these models. Furthermore, three implementation reverse genetic tools, yeast two-hybrid screening whole-genome transcriptome analysis revealed potential nuclear targets downstream triggered transcriptional responses. identified validated protein interactors participating host. S. demonstrated by transcriptomics effectors possible involvement different molecular processes, regulation DNA replication, methylglyoxal detoxification, RNA splicing. conclude R. may act on pathways modulate symbiosis discuss pros cons tools used.

Язык: Английский

Процитировано

0