Exploring the potential role of four Rhizophagus irregularis nuclear effectors: opportunities and technical limitations DOI Creative Commons
Maria Chacon,

Sofía Hernández Luelmo,

Viktor Devlieghere

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: April 24, 2024

Arbuscular mycorrhizal fungi (AMF) are obligate symbionts that interact with the roots of most land plants. The genome AMF model species Rhizophagus irregularis contains hundreds predicted small effector proteins secreted extracellularly but also into plant cells to suppress immunity and modify physiology establish a niche for growth. Here, we investigated role four nuclear-localized putative effectors, i.e. , GLOIN707, GLOIN781, GLOIN261, RiSP749, in mycorrhization We initially intended execute functional studies Solanum lycopersicum host economic interest not previously used biology, extended our Medicago truncatula as well non-host Arabidopsis thaliana because technical advantages working these models. Furthermore, three implementation reverse genetic tools, yeast two-hybrid screening whole-genome transcriptome analysis revealed potential nuclear targets downstream triggered transcriptional responses. identified validated protein interactors participating host. S. demonstrated by transcriptomics effectors possible involvement different molecular processes, regulation DNA replication, methylglyoxal detoxification, RNA splicing. conclude R. may act on pathways modulate symbiosis discuss pros cons tools used.

Language: Английский

Unconventional secretion of Magnaporthe oryzae effectors in rice cells is regulated by tRNA modification and codon usage control DOI
Gang Li, Nawaraj Dulal,

Ziwen Gong

et al.

Nature Microbiology, Journal Year: 2023, Volume and Issue: 8(9), P. 1706 - 1716

Published: Aug. 10, 2023

Language: Английский

Citations

23
Effectors and environment modulating rice blast disease: from understanding to effective control DOI
Yanjun Kou, Huanbin Shi, Jiehua Qiu

et al.

Trends in Microbiology, Journal Year: 2024, Volume and Issue: 32(10), P. 1007 - 1020

Published: April 5, 2024

Language: Английский

Citations

6
Characterization of Arbuscular Mycorrhizal Effector Proteins DOI Open Access
Maria Chacon, Judith Van Dingenen, Sofie Goormachtig

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(11), P. 9125 - 9125

Published: May 23, 2023

Plants are colonized by various fungi with both pathogenic and beneficial lifestyles. One type of colonization strategy is through the secretion effector proteins that alter plant's physiology to accommodate fungus. The oldest plant symbionts, arbuscular mycorrhizal (AMF), may exploit effectors their benefit. Genome analysis coupled transcriptomic studies in different AMFs has intensified research on function, evolution, diversification AMF. However, current 338 predicted from AM fungus

Language: Английский

Citations

14
Filamentous pathogen effectors enter plant cells via endocytosis DOI Creative Commons
Haixia Wang, Ely Oliveira‐Garcia, Petra C. Boevink

et al.

Trends in Plant Science, Journal Year: 2023, Volume and Issue: 28(11), P. 1214 - 1217

Published: Aug. 14, 2023

Recent findings demonstrate that cytoplasmic effectors from fungal and oomycete pathogens enter plant cells via clathrin-mediated endocytosis (CME). This raises several questions: Does effector secretion pathway facilitate host uptake? How is CME triggered in cells? are the released endosomal compartments to reach diverse subcellular destinations?

Language: Английский

Citations

12
The Dynamic Interaction between Oil Palm and Phytophthora palmivora in Bud Rot Disease: Insights from Transcriptomic Analysis and Network Modelling DOI Creative Commons
Mariandrea García-Gaona, David Botero-Rozo,

Leonardo Araque

et al.

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(3), P. 164 - 164

Published: Feb. 20, 2024

Bud Rot, caused by Phytophthora palmivora, is considered one of the main diseases affecting African oil palm (Elaeis guineensis). In this study, we investigated in vitro molecular dynamics pathogen–host interaction analyzing gene expression profiles from genotypes that were either susceptible or resistant to disease. We observed distinct interactions P. palmivora with and palms through co-expression network analysis. When interacting genotypes, exhibited upregulation carbohydrate sulfate transport genes. These genes demonstrated apoplastic cytoplasmic effectors, including cell wall degrading enzymes, elicitins, RxLR motif effectors. The pathogen manipulated materials, exacerbating response compromising phenylpropanoid pathway, ultimately leading susceptibility. contrast, materials control over their putative Heat Shock Proteins (HSP) maintained homeostasis between primary metabolism biotic defense. Co-expressed related flavonoids, WRKY transcripts, lectin-type receptors, LRR receptors may play important roles control. Overall, study provides new knowledge mechanisms underlying E. guineensis which can contribute controlling Rot gives insights into hosts.

Language: Английский

Citations

4
The secreted protein FonCHRD is essential for vegetative growth, asexual reproduction, and pathogenicity in watermelon Fusarium wilt fungus DOI Creative Commons

Jiajun Lou,

Jiajing Wang, Shaoqun Zeng

et al.

Crop Health, Journal Year: 2024, Volume and Issue: 2(1)

Published: Oct. 25, 2024

Abstract Fungal pathogens often secrete numerous effectors to interfere with and/or suppress plant immunity promote their infection. Watermelon Fusarium wilt, caused by the soil-borne fungus oxysporum f. sp. niveum ( Fon ), is one of devastating diseases that severely affect watermelon industry. Here, we report function a candidate effector protein, FonCHRD, in . FonCHRD harbors chordin (CHRD) domain unknown and has signal peptide secretion activity. shows relatively high expression level marcoconidia inducible root tissues. Phenotypic analysis targeted deletion mutant revealed plays roles vegetative growth, asexual reproduction, conidial morphology , while it not involved spore germination as well cell wall, oxidative salt stress responses. Deletion impaired ability colonize spread within host plants, significantly reducing its virulence on watermelon. distributed across multiple compartments cells but can target apoplast space plants. inhibits INF1- Bcl2-associated X protein-triggered death defense gene transiently expressed Nicotiana benthamiana leaves. These findings suggest essential for pathogenicity modulating invasive growth spreading abilities suppressing immune

Language: Английский

Citations

2
Paths of Least Resistance: Unconventional Effector Secretion by Fungal and Oomycete Plant Pathogens DOI Creative Commons
Nawaraj Dulal, Richard A. Wilson

Molecular Plant-Microbe Interactions, Journal Year: 2024, Volume and Issue: 37(9), P. 653 - 661

Published: July 1, 2024

Effector secretion by different routes mediates the molecular interplay between host plant and pathogen, but mechanistic details in eukaryotes are sparse. This may limit discovery of new effectors that could be utilized for improving disease resistance. In fungi oomycetes, apoplastic secreted via conventional endoplasmic reticulum (ER)-Golgi pathway, while cytoplasmic packaged into vesicles bypass Golgi an unconventional protein (UPS) pathway.

Language: Английский

Citations

1
Transformation‐based gene silencing and functional characterization of an ISC effector reveal how a powdery mildew fungus disturbs salicylic acid biosynthesis and immune response in the plant DOI Creative Commons

Jinyao Yin,

Xiao Li,

Longying Dong

et al.

Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 25(11)

Published: Nov. 1, 2024

Abstract Obligate biotrophic powdery mildew fungi infect a wide range of economically important plants. These often deliver effector proteins into the host tissues to suppress plant immunity and sustain infection. The phytohormone salicylic acid (SA) is one most signals that activate against pathogens. However, how effectors interact with SA signalling poorly understood. Isochorismatase (ISC) from two other filamentous pathogens have been found inhibit biosynthesis by hydrolysing isochorismate, main precursor in cytosol. Here, we identified an ISC effector, named EqIsc1, rubber tree fungus Erysiphe quercicola . In enzyme assays, EqIsc1 displayed activity transferring isochorismate 2,3‐dihydro‐2,3‐dihydroxybenzoate vitro transgenic Nicotiana benthamiana EqIsc1‐expressing Arabidopsis thaliana , SA‐mediated immune response were significantly inhibited. addition, developed electroporation‐mediated transformation method for genetic manipulation E. Inoculation leaves ‐silenced strain induced immunity. We also detected translocation cytosol during interaction between its host. Taken together, our results suggest functions as hydrolyse cytosol, altering response.

Language: Английский

Citations

1
Fusarium sacchari Effector FsMEP1 Contributes to Virulence by Disturbing Localization of Thiamine Thiazole Synthase ScTHI2 from Sugarcane DOI Open Access
Lulu Wang, Deng Wu,

Tianshu Hong

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12075 - 12075

Published: Nov. 10, 2024

is a significant pathogenic fungus that causes sugarcane Pokkah Boeng. Proteins secreted by fungi can be delivered into hosts to suppress plant immunity and establish infection. However, there still much discovered regarding

Language: Английский

Citations

0
Exploring the potential role of four Rhizophagus irregularis nuclear effectors: opportunities and technical limitations DOI Creative Commons
Maria Chacon,

Sofía Hernández Luelmo,

Viktor Devlieghere

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: April 24, 2024

Arbuscular mycorrhizal fungi (AMF) are obligate symbionts that interact with the roots of most land plants. The genome AMF model species Rhizophagus irregularis contains hundreds predicted small effector proteins secreted extracellularly but also into plant cells to suppress immunity and modify physiology establish a niche for growth. Here, we investigated role four nuclear-localized putative effectors, i.e. , GLOIN707, GLOIN781, GLOIN261, RiSP749, in mycorrhization We initially intended execute functional studies Solanum lycopersicum host economic interest not previously used biology, extended our Medicago truncatula as well non-host Arabidopsis thaliana because technical advantages working these models. Furthermore, three implementation reverse genetic tools, yeast two-hybrid screening whole-genome transcriptome analysis revealed potential nuclear targets downstream triggered transcriptional responses. identified validated protein interactors participating host. S. demonstrated by transcriptomics effectors possible involvement different molecular processes, regulation DNA replication, methylglyoxal detoxification, RNA splicing. conclude R. may act on pathways modulate symbiosis discuss pros cons tools used.

Language: Английский

Citations

0