State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Comprehensive Reviews in Food Science and Food Safety, Год журнала: 2022, Номер 21(2), С. 1868 - 1912

Опубликована: Фев. 22, 2022

Abstract Diverse chemicals and some physical phenomena recently introduced in nanotechnology have enabled scientists to develop useful devices the field of food sciences. Concerning such developments, detecting foodborne pathogenic bacteria is now an important issue. These kinds species demonstrated severe health effects after consuming foods high mortality related acute cases. The most leading path intoxication infection has been through matrices. Hence, quick recognition agents at low concentrations required current diagnostics. Lateral flow assays (LFAs) are one urgent prevalently applied methods that settled for recognizing diverse types analytes. Thus, present review stressed on latest developments LFAs‐based platforms detect various as Salmonella , Listeria Escherichia coli Brucella Shigella Staphylococcus aureus Clostridium botulinum Vibrio cholera . Proper prominence given exactly how labels, detection elements, or procedures affected recent evaluation using LFAs. Additionally, modifications specificity sensitivity consistent with processing techniques discussed. Finally, a conclusion drawn highlighting main challenges confronted this method offered view insight thoughts its further development future.

Язык: Английский

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Lateral flow assays (LFA) for detection of pathogenic bacteria: A small point-of-care platform for diagnosis of human infectious diseases

Talanta, Год журнала: 2022, Номер 243, С. 123330 - 123330

Опубликована: Фев. 22, 2022

Язык: Английский

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State-of-the-art cancer biomarker detection by portable (Bio) sensing technology: A critical review

Microchemical Journal, Год журнала: 2022, Номер 177, С. 107248 - 107248

Опубликована: Фев. 9, 2022

Язык: Английский

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Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance

Biosensors, Год журнала: 2023, Номер 13(9), С. 837 - 837

Опубликована: Авг. 23, 2023

Lateral flow tests are one of the most important types paper-based point-of-care (POCT) diagnostic tools. It shows great potential as an implement for improving rapid screening and management infections in global pandemics or other health disorders by using minimally expert staff locations where no sophisticated laboratory services accessible. They can detect different biomarkers various biological samples provide results a little time at low price. An challenge regarding conventional LFAs is increasing their sensitivity specificity. There two main approaches to increase specificity, including assay improvement target enrichment. Assay comprises optimization signal amplification techniques. In this study, summarize specificity enhancement strategies with objective evaluation presented, such detection element immobilization, capillary rate adjusting, label evolution, sample extraction enrichment, etc. also key findings LFA performance solving limitations discussed along numerous examples.

Язык: Английский

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Near-Infrared Responsive Ag@Au Nanoplates with Exceptional Stability for Highly Sensitive Colorimetric and Photothermal Dual-Mode Lateral Flow Immunoassay

Analytical Chemistry, Год журнала: 2024, Номер unknown

Опубликована: Фев. 5, 2024

Lateral flow immunoassay (LFIA) has played a vital role in point-of-care (POC) testing on account of its simplicity, rapidity, and low cost. However, the sensitivity difficulty quantitation limit further development. Sensitive markers with new detection modes are being developed to dramatically improve LFIA's performance. Herein, ligand-complex approach was proposed uniformly coat thin layer Au onto Ag triangular nanoplates (Ag TNPs) without etching cores, which not only retain unique optical properties from TNPs but also acquire surface stability biocompatibility gold. The localized plasmon resonance absorption these Ag@Au could be finely adjusted visible (550 nm) second near-infrared region (NIR-II) (1100 nm), even longer, by simply adjusting ratio between edge length thickness. Utilizing as for LFIA, highly sensitive colorimetric photothermal dual-mode SARS-CoV-2 nucleocapsid protein achieved very background. showed an exceedingly high conversion efficiency 61.4% (ca. 2 times higher than that nanorods), endowing LFIA method (40 pg/mL), 25-fold lower results. generality verified accurate analysis cardiac troponin I (cTnI). This is robust, reproducible, specific been successfully applied SARS-COV-2 35 clinical samples satisfactory results, demonstrating potential POC applications.

Язык: Английский

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Aptamer-Based Lateral Flow Assays: Current Trends in Clinical Diagnostic Rapid Tests

Pharmaceuticals, Год журнала: 2022, Номер 15(1), С. 90 - 90

Опубликована: Янв. 13, 2022

The lateral flow assay (LFA) is an extensively used paper-based platform for the rapid and on-site detection of different analytes. method user-friendly with no need sophisticated operation only includes adding sample. Generally, antibodies are employed as biorecognition elements in LFA. However, possess several disadvantages including poor stability, high batch-to-batch variation, long development time, price ethical approval cold chain. Because these limitations, aptamers screened by vitro process can be a good alternative to molecules In recent years, aptamer-based LFAs have been investigated analytes point-of-care diagnostics. this review, we summarize applications aptamer technology clinical diagnostic tests biomarkers, microbial analytes, hormones antibiotics. Performance, advantages drawbacks developed assays also discussed.

Язык: Английский

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Current and Perspective Sensing Methods for Monkeypox Virus

Bioengineering, Год журнала: 2022, Номер 9(10), С. 571 - 571

Опубликована: Окт. 18, 2022

The outbreak of the monkeypox virus (MPXV) in non-endemic countries is an emerging global health threat and may have economic impact if proactive actions are not taken. As shown by COVID-19 pandemic, rapid, accurate, cost-effective detection techniques play a pivotal role disease diagnosis control. Considering sudden multicountry MPXV outbreak, critical evaluation approaches would be timely addition to endeavors progress for control prevention. Herein, we evaluate current methods, discuss their pros cons, provide recommended solutions problems. We review traditional nucleic acid approaches, immunodiagnostics, whole-particle detection, imaging-based techniques. insights provided this article will help researchers develop novel MPXV.

Язык: Английский

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Specific lateral flow detection of isothermal nucleic acid amplicons for accurate point-of-care testing

Biosensors and Bioelectronics, Год журнала: 2022, Номер 222, С. 114989 - 114989

Опубликована: Дек. 15, 2022

Язык: Английский

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Self-assembled monolayer-assisted label-free electrochemical genosensor for specific point-of-care determination of Haemophilus influenzae

Microchimica Acta, Год журнала: 2023, Номер 190(4)

Опубликована: Март 4, 2023

Язык: Английский

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Lateral Flow Strip Biosensors for Foodborne Pathogenic Bacteria via Direct and Indirect Sensing Strategies: A Review

Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 71(27), С. 10250 - 10268

Опубликована: Июнь 30, 2023

Rapid and sensitive detection of foodborne pathogenic bacteria is particularly important for the prevention control diseases. The lateral flow strip biosensor (LFSB) one most promising point-of-care tools has been widely used in food safety monitoring. This review introduces recent advances using LFSBs. According to different bacterial biomarkers, we summarize direct indirect sensing strategies whole cells are divided into antibodies, antibody alternatives, label-free according recognition elements. refer nucleic acids metabolites. Next, compare discuss applications strategies. Finally, existing challenges, future perspectives, development directions discussed, which will facilitate theoretical innovation practical application

Язык: Английский

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