Applied Microbiology and Biotechnology,
Год журнала:
2025,
Номер
109(1)
Опубликована: Март 3, 2025
Snake
venom
has
long-term
physiological
effects
on
survivor's
life.
An
electrochemical
immunosensor
based
samarium-cobalt-doped
antimony
tungstate
(Sb2WO4@Sm-Co)
is
developed
via
a
solvothermal
method
to
detect
snake
antigens
(SVA).
The
fabricated
nanospheres
are
functionalized
with
carboxyl
groups
enhance
the
linkage
of
3-mercaptopropionic
acid
linker
(3-MPA).
This
modification
increases
conjugation
antivenom
polyvalent
antibody
nanomaterial
glassy
carbon
electrode
(Sb2WO4@Sm-Co-COOH-MPA-Ab/GCE).
modified
characterized
by
UV–VIS
spectroscopy,
Fourier
transform
infrared
spectroscopy
(FTIR),
scanning
electron
microscopy
(SEM),
transmission
(TEM),
and
energy
dispersive
X-ray
(EDS).
performance
formulated
for
antigen
sensing
tested
cyclic
voltammetry
(CV),
impedance
(EIS),
differential
pulse
(DPV),
linear
sweep
(LSV),
chronoamperometry.
wide
range
5–30
ng/mL
LODs
0.08
0.1
from
DPV
LSV,
respectively.
amperometric
antibody's
loading
capacity
accelerates
transfer
rate.
analytical
parameters
reveal
that
this
ultrasensitive,
stable,
reproducible,
selective
measuring
SVA
can
have
potential
applications
in
diagnostic
clinics.
•
hierarchical
Sb2WO4@Sm-Co-COOH
NPs
were
synthesized
through
one-step
Monitoring
effect
doping
Sm
Co
characteristics
Sb2WO4
MPA-linked
IgG
antibodys-based
was
good
dispersity
high
surface
functional
capturing
SVAs
Abstract
Triboelectric
nanogenerators
(TENGs)
can
collect
and
convert
random
mechanical
energy
into
electric
energy,
with
remarkable
advantages
including
broadly
available
materials,
straightforward
preparation,
multiple
applications.
Over
the
years,
researchers
have
made
substantial
advancements
in
theoretical
practical
aspects
of
TENG.
Nevertheless,
pivotal
challenge
realizing
full
applications
TENG
lies
ensuring
that
generated
output
meets
specific
application
requirements.
Consequently,
research
is
dedicated
to
exploring
methods
mechanisms
for
enhancing
performance
devices.
This
review
aims
comprehensively
examine
influencing
factors
corresponding
improvement
strategies
based
on
contact
electrification
mechanism
operational
principles
underlie
technology.
primarily
delves
five
key
areas
improvement:
materials
selection,
surface
modification,
component
adjustments,
structural
optimization,
electrode
enhancements.
These
are
crucial
tailoring
devices
meet
desired
metrics
various
Abstract
Circulating
microRNAs
(miRNAs)
can
be
used
as
noninvasive
biomarkers
and
are
also
found
circulating
in
body
fluids
such
blood.
Dysregulated
miRNA
expression
is
associated
with
many
diseases,
including
non‐small
cell
lung
cancer
(NSCLC),
the
assay
helpful
diagnosis,
prognosis,
monitoring.
In
this
work,
a
versatile
electrochemical
biosensing
system
developed
for
detection
by
DNAzyme‐cleavage
cycling
amplification
hybridization
chain
reaction
(HCR)
amplification.
With
cleavage
Mn
2+
targeted
DNAzyme,
DNA‐walker
move
along
predesigned
DNA
tracks
contribute
to
transduction
enhancement
of
signals.
For
process,
formation
multiple
G‐quadruplex‐incorporated
long
double‐stranded
(dsDNA/G‐quadruplex)
structures
triggered
through
HCR
The
introduction
G‐quadruplex
allows
sensitive
measurement
down
5.68
fM
good
specificity.
Furthermore,
profiling
NSCLC
cohort,
designed
strategy
shows
high
efficiency
(area
under
curve
(AUC)
0.879
using
receiver
operating
characteristic
(ROC)
analysis)
sensitivity
80.0%
early
diagnosis
(stage
I).
discrimination
benign
disease,
displays
an
AUC
0.907,
superior
six
clinically‐acceptable
protein
tumor
markers.
Therefore,
platform
holds
promise
clinical
application
toward
prognosis.
Abstract
Exosomes
are
promising
new
biomarkers
for
colorectal
cancer
(CRC)
diagnosis,
due
to
their
rich
biological
fingerprints
and
high
level
of
stability.
However,
the
accurate
detection
exosomes
with
specific
surface
receptors
is
limited
clinical
application.
Herein,
an
exosome
enrichment
platform
on
a
3D
porous
sponge
microfluidic
chip
constructed
capture
efficiency
this
≈90%.
Also,
deep
mass
spectrometry
analysis
followed
by
multi‐level
expression
screenings
revealed
CRC‐specific
membrane
protein
(SORL1).
A
method
SORL1
quantum
dot
labeling
further
designed
ensemble
classification
system
established
extracting
features
from
64‐patched
fluorescence
images.
Importantly,
area
under
curve
(AUC)
using
0.99,
which
significantly
higher
(
p
<
0.001)
than
that
conventional
biomarker
(carcinoembryonic
antigen
(CEA),
AUC
0.71).
The
above
showed
similar
diagnostic
performance,
dealing
early‐stage
CRC,
young
CEA‐negative
CRC
patients.
Abstract
Accurate
and
rapid
metabolic
profiling
of
cerebrospinal
fluid
(CSF)
is
urgently
needed
but
remains
challenging
for
clinical
diagnosis
central
nervous
system
diseases
biomarker
discovery.
Matrix‐assisted
laser
desorption
ionization
mass
spectrometry
(MALDI‐MS)
holds
promise
analysis.
Its
low
signal
reproducibility,
however,
severely
restricts
acquisition
quantitative
MS
data
in
practice.
Herein,
a
multifunctional
self‐assembled
AuNPs
array
(MSANA)‐based
LDI‐MS
platform
direct
amino
acids
analysis
patient
CSF
samples
developed.
MSANA
featuring
highly
ordered
closely
packed
two‐dimensional
nanostructure
permits
capture
aromatic
by
with
high
selectivity
micromolar
sensitivity.
Meanwhile,
the
MSANA‐based
exhibits
excellent
reproducibility
(RSD
<
10%),
largely
outperforming
matrix
spotting
approach
widely
used
now
44%).
The
successfully
(1
µL)
within
minutes
discrimination
medulloblastoma
patients
from
non‐tumor
controls.
Taken
together,
shows
potential
values
toward
large‐scale
diagnostics
pathogenic
mechanism
study.
Abstract
Efficient
diagnosis
of
mycobacterial
infections
can
effectively
manage
and
prevent
the
transmission
infectious
diseases.
Unfortunately,
existing
diagnostic
strategies
are
challenged
by
long
assay
times,
high
costs,
highly
specialized
expertise
to
distinguish
between
pulmonary
tuberculosis
(PTB)
nontuberculous
diseases
(NTM‐PDs).
Herein,
in
this
study,
an
optimized
3D
paper‐based
analytical
device
(µPAD)
is
incorporated
with
a
closed
lateral
flow
(LF)
strip
into
loop‐mediated
isothermal
amplification
(LAMP)
(3D‐µPAD‐LF‐LAMP)
for
rapid,
low‐cost,
visual
detection
pathogenic
mycobacteria.
The
platform's
microfluidic
feature
enhanced
nucleic
acid
amplification,
thereby
reducing
costs
time
as
compared
boiling,
easyMAG,
QIAGEN
techniques.
Moreover,
LF
unit
specifically
designed
minimize
aerosol
contamination
user‐friendly
readout.
3D‐µPAD‐LF‐LAMP
assessed
using
standard
strains,
demonstrating
limit
(LOD)
down
10
fg
reaction
−1
.
In
cohort
815
patients,
displays
significantly
better
sensitivity,
specificity,
negative
predictive
value
(NPV),
positive
(PPV),
accuracy
than
conventional
bacterial
culture
Xpert
Collectively,
demonstrates
accessibility,
efficiency,
practicality
multiple
mycobacteria,
which
be
applied
across
diverse
clinical
settings,
ultimately
improving
public
health
outcomes.
Abstract
Detecting
exosomal
markers
using
laser
desorption/ionization
time‐of‐flight
mass
spectrometry
(LDI‐TOF
MS)
is
a
novel
approach
for
examining
liquid
biopsies
of
non‐small
cell
lung
cancer
(NSCLC)
samples.
However,
LDI‐TOF
MS
limited
by
low
sensitivity
and
poor
reproducibility
when
analyzing
intact
proteins
directly.
In
this
report,
gold
nanoparticles/cellulose
nanocrystals
(AuNPs/CNC)
introduced
as
the
matrix
direct
analysis
in
NSCLC
serum
exosomes.
AuNPs/CNC
with
“dual
dispersion”
effects
dispersed
stabilized
AuNPs
improved
ion
inhibition
caused
protein
aggregation.
These
features
increased
signal‐to‐noise
ratio
[M+H]
+
peaks
two
orders
magnitude
lowered
detection
limit
to
0.01
mg
mL
–1
.
The
coefficient
variation
or
without
measured
10.2%
32.5%,
respectively.
excellent
yielded
linear
relationship
(
y
=
15.41
x
–
7.983,
R
2
0.989)
over
concentration
range
20
Finally,
AuNPs/CNC‐assisted
provides
clinically
relevant
fingerprint
information
serum,
characteristic
S100
calcium‐binding
A10,
Urokinase
plasminogen
activator
surface
receptor,
Plasma
protease
C1
inhibitor,
Tyrosine‐protein
kinase
Fgr
Mannose‐binding
lectin
associated
serine
represented
predictive
biomarkers
risk.