Advanced Materials,
Год журнала:
2023,
Номер
36(22)
Опубликована: Ноя. 28, 2023
Abstract
Extracellular
vesicles
(EVs)
are
cell‐derived
nanovesicles
comprising
a
myriad
of
molecular
cargo
such
as
proteins
and
nucleic
acids,
playing
essential
roles
in
intercellular
communication
physiological
pathological
processes.
EVs
have
received
substantial
attention
noninvasive
biomarkers
for
disease
diagnosis
prognosis.
Owing
to
their
ability
recognize
protein
acid
targets,
DNA‐based
nanomaterials
with
excellent
programmability
modifiability
provide
promising
tool
the
sensitive
accurate
detection
carried
by
EVs.
In
this
perspective,
recent
advancements
EV
analysis
using
variety
summarized,
which
can
be
broadly
classified
into
three
categories:
linear
DNA
probes,
nanostructures,
hybrid
nanomaterials.
The
design,
construction,
advantages,
disadvantages
different
types
nanomaterials,
well
performance
detecting
reviewed.
challenges
opportunities
field
also
discussed.
ACS Nano,
Год журнала:
2024,
Номер
18(46), С. 31713 - 31736
Опубликована: Ноя. 7, 2024
Sensitive
and
quantitative
detection
of
chemical
biological
molecules
for
screening,
diagnosis
monitoring
diseases
is
essential
to
treatment
planning
response
monitoring.
Electrochemical
biosensors
are
fast,
sensitive,
easy
miniaturize,
which
has
led
rapid
development
in
clinical
diagnosis.
Benefiting
from
their
excellent
molecular
recognition
ability
high
programmability,
DNA
nanomaterials
could
overcome
the
Debye
length
electrochemical
by
simple
design
well
suited
as
elements
biosensors.
Therefore,
enhance
sensitivity
specificity
biosensors,
significant
progress
been
made
recent
years
optimizing
design.
Here,
establishment
sensing
strategies
based
on
reviewed
detail.
First,
structural
nanomaterial
examined
improving
overcoming
length.
In
addition,
electrical
signal
transduction
amplification
reviewed,
applications
nanomaterial-based
integrated
devices
further
summarized.
Finally,
main
opportunities
challenges
detecting
disease
biomarkers
presented
an
aim
guide
with
specificity.
Biosensors,
Год журнала:
2022,
Номер
12(12), С. 1094 - 1094
Опубликована: Ноя. 29, 2022
Paper-based
biosensors
are
microfluidic
analytical
devices
used
for
the
detection
of
biochemical
substances.
The
unique
properties
paper-based
biosensors,
including
low
cost,
portability,
disposability,
and
ease
use,
make
them
an
excellent
tool
point-of-care
testing.
Among
all
analyte
methods,
nucleic
acid-based
pathogen
offers
versatility
due
to
acid
synthesis.
In
a
testing
context,
combination
platform
allows
accurate
detection.
This
review
overview
contemporary
detecting
acids
from
pathogens.
methods
limitations
implementing
integrated
portable
discussed.
concludes
with
potential
directions
future
research
in
development
biosensors.
Herein,
for
the
first
time,
CRISPR-Cas12a
system
is
combined
with
aptamer,
cascaded
dynamic
DNA
network
circuits,
and
Fe3
O4
@hollow-TiO2
@MoS2
nanochains
(Fe3
@h-TiO2
NCs)
to
construct
an
efficient
sensing
platform
tetracycline
(TC)
analysis.
In
this
strategy,
specific
recognition
of
target
transduced
amplified
into
H1-H2
duplexes
containing
sequence
Cas12a-crRNA
through
aptamer
module
dual
amplification
network.
Subsequently,
obtained
activated
Cas12a
protein
non-specifically
cleaves
adjacent
reporter
gene
ssDNA-FAM
dissociate
FAM
molecule
from
quencher
NCs,
resulting
in
recovery
fluorescence
signal
further
amplification.
Particularly,
synthesized
multifunctional
NCs
composites
also
exhibit
superb
magnetic
separability
photocatalytic
degradation
ability.
Under
optimal
conditions,
aptasensor
displays
a
distinct
linear
relationship
logarithm
TC
concentration,
limit
detection
as
low
0.384
pg
mL-1
.
Furthermore,
results
spiked
confirm
viability
proposed
quantification
real
samples.
This
study
extends
application
field
analytical
contributes
new
insights
exploration
reliable
tools
monitoring
treating
hazards
food
environment.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(30), С. 11187 - 11192
Опубликована: Июль 18, 2023
In
this
technical
note,
we
report
an
easy-to-produce,
reverse-transcription-free,
and
protein-enzyme-free
lateral
flow
assay
for
detection
of
viral
RNA
fragments
by
taking
SARS-CoV-2
ORF1ab
N
as
target
models.
Catalytic
hairpin
assembly
is
utilized
dual
fragment
orthogonal
reaction
to
generate
copious
amounts
opened
duplexes,
which
bridge
DNA-modified
gold
nanoparticles
capture
strands
on
the
strip
induce
coloration.
The
are
simultaneously
visualized
during
one
time
sample
flow,
single-base-mismatched
nontarget
sequences
can
be
differentiated.
test
flexibly
adapted
detect
evolutional
variants
such
Delta
Omicron.
It
also
shows
potential
in
visually
detecting
long-sequence
virus
simulants
achieves
a
sensitivity
comparable
that
RT-qPCR
incorporation
with
upstream
amplification.
should
offer
convenient
reliable
technique
nucleic
acid
detection.
