ACS Sensors,
Год журнала:
2024,
Номер
9(10), С. 5302 - 5311
Опубликована: Окт. 10, 2024
The
development
of
stimulus-responsive
and
amplification-based
strategies
is
crucial
for
achieving
improved
spatial
specificity
enhanced
sensitivity
in
tumor
molecular
imaging,
addressing
challenges
such
as
off-tumor
signal
leakage
limited
biomarker
content.
Therefore,
a
cyclically
activated
enzymatic
biosensor
based
on
the
modification
an
AP
site
within
tetrahedral
framework
DNA
(AP-tFNA)
was
rationally
developed
cell-specific
imaging
using
endogenous
enzyme
apurinic/apyrimidinic
endonuclease
1
(APE1)
target,
exhibiting
superior
high
sensitivity.
APE1,
which
predominantly
localizes
nucleus
normal
cells
but
exhibits
cytosolic
expression
cancer
cells,
can
specifically
recognize
cleave
AP-tFNA,
resulting
separation
fluorophore
quenching
group,
thereby
inducing
fluorescence
signal.
Additionally,
upon
completion
excision
one
APE1
released,
initiating
subsequent
cycle
hydrolytic
cleavage
reactions.
experimental
results
demonstrated
that
AP-tFNA
enables
precise
differentiation
both
Abstract
Intelligent
point‐of‐care
testing
(iPOCT)
is
a
rapidly
advancing
technology
that
combines
molecular
detection
and
bio‐sensing
techniques
to
achieve
instant
accurate
analysis
through
advanced
sensors
intelligent
algorithms.
Molecular
technologies,
such
as
nucleic
acid
isothermal
amplification,
biochip
microfluidics,
enable
rapid
of
the
chemical
composition
concentration
samples,
thereby
determining
their
presence.
Bio‐sensing
utilizes
recognition
properties
biomolecules
in
conjunction
with
detect
specific
or
biological
processes.
iPOCT
holds
broad
prospects
for
application
field
healthcare
expected
enhance
sensitivity,
accuracy,
intelligence
further
development.
In
this
review,
we
delineate
developmental
journey
iPOCT,
followed
by
discussion
on
principles
technology,
well
design
devices
health
monitoring
disease
diagnosis.
Furthermore,
showcased
illustrative
examples
applications
diagnosis,
highlighting
integration
wearable
smartphones
attaining
more
precise
personalized
diagnostic
results.
Journal of the American Chemical Society,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 11, 2025
MicroRNA
(miRNA)
is
involved
in
the
genesis
viand
development
of
colorectal
cancer.
The
vivo
imaging
miRNA
at
tumor
sites
essential
for
understanding
its
role
cancer
pathology
and
therapeutic
target
identification.
However,
achieving
accurate
hindered
by
low
abundance
miRNAs
cells
nonspecific
signal
leakage
normal
tissues.
Here,
we
report
a
multivariate-gated
catalytic
hairpin
assembly
(CHA)
nanosensor
specific
amplified
microRNA-21
(miR-21)
human
tissues
to
reveal
underlying
miR-21-associated
molecular
mechanism.
endogenous
glutathione
exogenous
near-infrared
design
combination
with
CHA
probes
improves
strength
miR-21
reduces
background
interference.
enables
vivo,
signal-to-background
ratios
are
1.6-fold
compared
traditional
methods.
With
assistance
designed
nanosensor,
achieve
preliminary
identification
from
clinical
surgical
resection
samples.
overexpressed
found
suppress
core
mismatch
repair
recognition
protein
mutS
homologue
2
DNA
damage
inhibit
efficacy
strategy
probe
design,
which
combines
activation
methods
amplification
system,
applicable
disease-relevant
mechanism
research.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(22), С. 9078 - 9087
Опубликована: Май 21, 2024
As
an
important
disease
biomarker,
the
development
of
sensitive
detection
strategies
for
miRNA,
especially
intracellular
miRNA
imaging
strategies,
is
helpful
early
diagnosis
diseases,
pathological
research,
and
drug
development.
Hybridization
chain
reaction
(HCR)
widely
used
analysis
because
its
high
specificity
lack
biological
enzymes.
However,
classic
HCR
exhibits
linear
amplification
with
low
efficiency,
limiting
use
rapid
trace
in
living
cells.
To
address
this
problem,
we
proposed
a
toehold-mediated
exponential
(TEHCR)
to
achieve
highly
efficient
cells
using
β-FeOOH
nanoparticles
as
transfection
vectors.
The
limit
TEHCR
was
92.7
fM,
which
8.8
×
103
times
lower
compared
traditional
HCR,
it
can
effectively
distinguish
single-base
mismatch
specificity.
also
different
expression
levels
cancer
normal
Furthermore,
be
construct
OR
logic
gates
dual
without
need
additional
probes,
demonstrating
flexibility.
This
method
expected
play
role
clinical
miRNA-related
well
promote
gates.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(32), С. 13299 - 13307
Опубликована: Авг. 2, 2024
Exosomes
have
received
considerable
attention
as
potent
reference
markers
for
the
diagnosis
of
various
neoplasms
due
to
their
close
and
direct
relationship
with
proliferation,
adhesion,
migration
tumor.
The
ultrasensitive
detection
cancer-derived
low-abundance
exosomes
is
imperative,
but
still
a
great
challenge.
Herein,
we
report
an
electrochemiluminescence
(ECL)
biosensor
based
on
DNA-bio-bar-code
hybridization
chain
reaction
(HCR)-mediated
dual
signal
amplification
exosomes.
In
this
system,
two
types
aptamers
were
modified
magnetic
nanoprobe
(MNPs)
gold
nanoparticles
(AuNPs)
numerous
bio-bar-code
DNA,
respectively,
which
formed
"sandwich"
structures
in
presence
specific
target
separated
under
field,
DNA
released
by
dissolving
AuNPs.
triggered
HCR
procedure
produce
good
deal
long
duplex
structure
embedding
hemin,
generated
strong
ECL
coreactors
Under
optimal
conditions,
it
exhibited
linearly
ranging
from
10
Analytical Chemistry,
Год журнала:
2024,
Номер
96(19), С. 7550 - 7557
Опубликована: Май 6, 2024
Developing
precise
tumor
cell-specific
mitochondrial
ferroptosis-related
inhibition
miRNA
imaging
methods
holds
enormous
potential
for
anticancer
drug
screening
and
cancer
treatment.
Nevertheless,
traditional
amplification
still
tolerated
the
limited
specificity
because
of
"off-tumor"
signal
leakage
resulting
from
their
"always-active"
sensing
mode.
To
overcome
this
limitation,
we
herein
developed
a
dual
(exogenous
808
nm
NIR
light
endogenous
APE1)
activated
nanoladder
with
cell
improved
resolution.
Exogenous
light-activation
can
regulate
signals
within
mitochondria,
enzyme-activation
confine
to
cells.
Based
on
activation
design,
off-tumor
were
greatly
reduced
tumor-to-background
contrast
was
enhanced
an
tumor/normal
discrimination
ratio,
realizing
miRNA.
Analytical Chemistry,
Год журнала:
2023,
Номер
95(27), С. 10330 - 10336
Опубликована: Июнь 27, 2023
Small
extracellular
vesicles
(sEVs)
derived
from
tumors
contain
a
vast
amount
of
cellular
information
and
are
regarded
as
potential
diagnostic
biomarker
for
noninvasive
cancer
diagnosis.
Nevertheless,
it
remains
challenging
to
accurately
measure
sEVs
clinical
samples
due
the
low
abundance
these
well
their
phenotypic
heterogeneity.
Herein,
polymerase-driven
logic
signal
amplification
system
(PLSAS)
was
developed
high-sensitivity
detection
sEV
surface
proteins
breast
(BC)
identification.
Aptamers
were
introduced
serve
sensing
modules
specifically
recognize
target
proteins.
By
changing
input
DNA
sequences,
two
primer
exchange
reaction
systems
rationally
designed
computing.
This
allows
autonomous
targeting
limited
number
targets
using
"OR"
"AND"
logic,
leading
significant
increase
in
fluorescence
signals
enabling
specific
ultrasensitive
In
this
work,
we
investigated
mucin
1
(MUC1)
epithelial
cell
adhesion
molecule
(EpCAM)
model
When
MUC1
or
EpCAM
used
single
system,
limit
24
58
particles/μL,
respectively.
And
can
be
simultaneously
detected
AND
method,
which
significantly
reduce
effect
heterogeneity
distinguish
source
various
mammary
lines,
such
MCF-7,
MDA
MB
231,
SKBR3,
MCF-10A.
The
approach
has
achieved
high
discrimination
serologically
tested
positive
BC
(AUC
98.1%)
holds
advancing
early
diagnosis
prognostic
assessments
BC.
