Biosensors,
Год журнала:
2024,
Номер
14(12), С. 633 - 633
Опубликована: Дек. 20, 2024
Antibiotics,
celebrated
as
some
of
the
most
significant
pharmaceutical
breakthroughs
in
medical
history,
are
capable
eliminating
or
inhibiting
bacterial
growth,
offering
a
primary
defense
against
wide
array
infections.
However,
rise
antimicrobial
resistance
(AMR),
driven
by
widespread
use
antibiotics,
has
evolved
into
and
ominous
threat
to
global
public
health.
Thus,
creation
efficient
methods
for
detecting
genes
antibiotics
is
imperative
ensuring
food
safety
safeguarding
human
The
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
CRISPR-associated
proteins
(Cas)
systems,
initially
recognized
an
adaptive
immune
mechanism
bacteria
archaea,
have
unveiled
their
profound
potential
sensor
detection,
transcending
notable
gene-editing
applications.
CRISPR/Cas
technology
employs
Cas
enzymes
guides
RNA
selectively
target
cleave
specific
DNA
sequences.
This
review
offers
extensive
examination
highlighting
unique
attributes
applications
antibiotic
detection.
It
outlines
current
utilization
progress
toolkit
identifying
both
nucleic
acid
(resistance
genes)
non-nucleic
(antibiotic
micromolecules)
targets
within
field
In
addition,
it
examines
challenges,
such
sensitivity
specificity,
future
opportunities,
including
development
point-of-care
diagnostics,
providing
strategic
insights
facilitate
curbing
oversight
antibiotic-resistance
proliferation.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(17), С. 6853 - 6859
Опубликована: Апрель 22, 2024
The
presence
of
small
molecule
contaminants
such
as
mycotoxins
and
heavy
metals
in
foods
the
environment
causes
a
serious
threat
to
human
health
huge
economic
losses.
development
simple,
rapid,
sensitive,
on-site
methods
for
pollutant
detection
is
highly
demanded.
Here,
combining
advantages
structure-switchable
aptamer-mediated
signal
conversion
CRISPR/Cas12a-based
amplification,
we
developed
CRISPR/Cas12a-amplified
aptamer
switch
assay
on
microplate
sensitive
detection.
In
this
assay,
short
DNA
strand
complementary
(cDNA)
immobilized
microplate,
which
can
capture
aptamer-linked
active
probe
(Apt-acDNA)
sample
solution
when
target
absent.
With
addition
Cas12a
reporter
system,
captured
Apt-acDNA
probes
activate
indiscriminately
cleave
fluorescent
substrates,
producing
high
fluorescence
signal.
When
present,
specifically
binds
rather
than
hybridizing
with
cDNA
reduced.
analytical
performance
our
method
was
demonstrated
by
two
toxic
pollutants,
aflatoxin
B1
(AFB1)
cadmium
ion
(Cd
Journal of Genetic Engineering and Biotechnology,
Год журнала:
2024,
Номер
22(3), С. 100400 - 100400
Опубликована: Июль 26, 2024
Aptamers
are
single-stranded
oligonucleotide
sequences
capable
of
binding
to
specific
ligands
with
high
affinity.
In
this
manner,
they
like
antibodies
but
have
advantages
such
as
lower
manufacturing
costs,
immunogenicity,
fewer
batch-to-batch
differences,
a
longer
shelf
life,
tolerance
different
molecular
milieus,
and
greater
number
potential
targets.
Due
their
special
features,
been
used
in
drug
delivery,
biosensor
technology,
therapy,
diagnostics.
The
methodology
that
allowed
its
production
was
the
"Systematic
Evolution
Ligands
by
Exponential
enrichment"
(SELEX).
Unfortunately,
traditional
protocol
is
time-consuming
laborious.
Therefore,
numerous
variants
considerable
optimization
steps
developed,
nonetheless,
there
still
challenges
achieving
real
applications
clinical
field.
Among
them,
control
vivo
activities,
fast
renal
filtration,
degradation
nucleases
toxicity
testing.
This
review
focuses
on
current
technologies
based
SELEX,
critical
factors
for
successful
aptamer
selection,
upcoming
biomedical
biotechnological
applications.
ACS Synthetic Biology,
Год журнала:
2025,
Номер
14(1), С. 21 - 40
Опубликована: Янв. 6, 2025
The
field
of
healthcare
diagnostics
is
navigating
complex
challenges
driven
by
evolving
patient
demographics
and
the
rapid
advancement
new
technologies
worldwide.
In
response
to
these
challenges,
biosensors
offer
distinctive
advantages
over
traditional
diagnostic
methods,
such
as
cost-effectiveness,
enhanced
specificity,
adaptability,
making
their
integration
with
point-of-care
(POC)
platforms
more
feasible.
recent
years,
aptasensors
have
significantly
evolved
in
capabilities
through
emerging
microfluidics,
Clustered
Regularly
Interspaced
Short
Palindromic
Repeats
(CRISPR)
systems,
wearable
devices,
machine
learning
(ML),
driving
progress
precision
medicine
global
solutions.
Moreover,
advancements
not
only
improve
accuracy
but
also
hold
potential
revolutionize
early
detection,
reduce
costs,
outcomes,
especially
resource-limited
settings.
This
Account
examines
key
advancements,
focusing
on
how
scientific
breakthroughs,
including
artificial
intelligence
(AI),
improved
sensitivity
precision.
Additionally,
has
enabled
real-time
monitoring
data
analysis,
fostering
advances
personalized
healthcare.
Furthermore,
commercialization
aptasensor
could
increase
availability
clinical
settings
support
use
widespread
solutions
for
health
challenges.
Hence,
this
review
discusses
technological
improvements,
practical
uses,
prospects
while
surrounding
standardization,
validation,
interdisciplinary
collaboration
application.
Finally,
ongoing
efforts
address
are
ensure
that
can
be
effectively
implemented
diverse
systems.
