Biosensors,
Год журнала:
2024,
Номер
14(12), С. 633 - 633
Опубликована: Дек. 20, 2024
Antibiotics,
celebrated
as
some
of
the
most
significant
pharmaceutical
breakthroughs
in
medical
history,
are
capable
eliminating
or
inhibiting
bacterial
growth,
offering
a
primary
defense
against
wide
array
infections.
However,
rise
antimicrobial
resistance
(AMR),
driven
by
widespread
use
antibiotics,
has
evolved
into
and
ominous
threat
to
global
public
health.
Thus,
creation
efficient
methods
for
detecting
genes
antibiotics
is
imperative
ensuring
food
safety
safeguarding
human
The
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
CRISPR-associated
proteins
(Cas)
systems,
initially
recognized
an
adaptive
immune
mechanism
bacteria
archaea,
have
unveiled
their
profound
potential
sensor
detection,
transcending
notable
gene-editing
applications.
CRISPR/Cas
technology
employs
Cas
enzymes
guides
RNA
selectively
target
cleave
specific
DNA
sequences.
This
review
offers
extensive
examination
highlighting
unique
attributes
applications
antibiotic
detection.
It
outlines
current
utilization
progress
toolkit
identifying
both
nucleic
acid
(resistance
genes)
non-nucleic
(antibiotic
micromolecules)
targets
within
field
In
addition,
it
examines
challenges,
such
sensitivity
specificity,
future
opportunities,
including
development
point-of-care
diagnostics,
providing
strategic
insights
facilitate
curbing
oversight
antibiotic-resistance
proliferation.
ACS Sensors,
Год журнала:
2023,
Номер
9(1), С. 244 - 250
Опубликована: Дек. 12, 2023
CRISPR-mediated
aptasensors
have
gained
prevalence
for
detecting
non-nucleic
acid
targets.
However,
there
is
an
urgent
need
to
develop
easily
customizable
design
improve
the
signal-to-noise
ratio,
enhance
universality,
and
expand
detection
range.
In
this
article,
we
report
a
programmable
aptasensor
(CPAS)
platform.
The
platform
includes
single-stranded
DNA
comprising
aptamer
sequence,
locker
DNA,
crRNA
recognition
region,
forming
hairpin
structure
through
complementary
hybridization.
With
T4
polymerase,
region
was
transformed
into
complete
double-stranded
stem-loop
extension,
thereby
activating
trans-cleavage
activity
of
Cas
12a
generating
fluorescence
signals.
specific
binding
between
target
molecule
disrupted
formation
structure,
altering
Notably,
CPAS
allows
easy
customization
by
simply
changing
sequence
without
entailing
adjustments
crRNA.
optimal
number
bases
in
determined
be
seven
nucleotides
SARS-CoV-2
spike
(S)
protein
four
ATP.
exhibited
high
sensitivity
S
ATP
detection.
Integration
with
lateral
flow
assay
enabled
sensitive
within
1
h,
revealing
its
excellent
potential
portable
analysis.
Advanced Functional Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 26, 2025
Abstract
Single‐atom
nanozyme
(SAZ)
with
peroxidase‐like
activity
has
attracted
great
attention
in
point‐of‐care
(POC)
diagnostic,
but
the
use
of
unstable
H
2
O
peroxidase
catalytic
reactions
often
leads
to
low
detection
accuracy.
Thus,
developing
SAZ
high
oxidase
(OD)‐like
construct
RNA
sensing
systems
independent
is
imperative
for
improving
Herein,
a
novel
strategy
fabricate
boron‐nitrogen
co‐doped
zinc
(ZnBNC‐SAZ)
excellent
OD‐like
by
carbonizing
Zn
based
zeolitic
boron
imidazolate
frameworks
reported.
The
electron
deficient
B
ligand
can
form
Zn─N─B
bond
situ
during
temperature
pyrolysis,
which
regulate
electronic
structure
and
further
upshift
d‐band
center
improve
activity.
With
ZnBNC‐SAZ
as
signal
generator,
new
method
sensitive
developed
combining
CRISPR/
cas13
system
terminal
deoxynucleotidyl
transferase‐induced
DNA
extension
reaction.
limits
RNAs
are
20
aM.
proposed
biosensor
adaptable
lateral‐flow‐based
readout
universally
applicable
various
programming
guide
RNA.
Importantly,
monitor
cellular
differentiation
identify
patients
cervical
carcinoma,
showing
potential
application
facile
POC
diagnosis.
