Mitigating Antibiotic Resistance: The Utilization of CRISPR Technology in Detection DOI Creative Commons

Xuejiao Zhang,

Z. Y. Huang,

Yanxia Zhang

и другие.

Biosensors, Год журнала: 2024, Номер 14(12), С. 633 - 633

Опубликована: Дек. 20, 2024

Antibiotics, celebrated as some of the most significant pharmaceutical breakthroughs in medical history, are capable eliminating or inhibiting bacterial growth, offering a primary defense against wide array infections. However, rise antimicrobial resistance (AMR), driven by widespread use antibiotics, has evolved into and ominous threat to global public health. Thus, creation efficient methods for detecting genes antibiotics is imperative ensuring food safety safeguarding human The clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated proteins (Cas) systems, initially recognized an adaptive immune mechanism bacteria archaea, have unveiled their profound potential sensor detection, transcending notable gene-editing applications. CRISPR/Cas technology employs Cas enzymes guides RNA selectively target cleave specific DNA sequences. This review offers extensive examination highlighting unique attributes applications antibiotic detection. It outlines current utilization progress toolkit identifying both nucleic acid (resistance genes) non-nucleic (antibiotic micromolecules) targets within field In addition, it examines challenges, such sensitivity specificity, future opportunities, including development point-of-care diagnostics, providing strategic insights facilitate curbing oversight antibiotic-resistance proliferation.

Язык: Английский

Programmable Aptasensor for Regulating CRISPR/Cas12a Activity DOI
Yao Yu, Qiaoyu Li, Wen Shi

и другие.

ACS Sensors, Год журнала: 2023, Номер 9(1), С. 244 - 250

Опубликована: Дек. 12, 2023

CRISPR-mediated aptasensors have gained prevalence for detecting non-nucleic acid targets. However, there is an urgent need to develop easily customizable design improve the signal-to-noise ratio, enhance universality, and expand detection range. In this article, we report a programmable aptasensor (CPAS) platform. The platform includes single-stranded DNA comprising aptamer sequence, locker DNA, crRNA recognition region, forming hairpin structure through complementary hybridization. With T4 polymerase, region was transformed into complete double-stranded stem-loop extension, thereby activating trans-cleavage activity of Cas 12a generating fluorescence signals. specific binding between target molecule disrupted formation structure, altering Notably, CPAS allows easy customization by simply changing sequence without entailing adjustments crRNA. optimal number bases in determined be seven nucleotides SARS-CoV-2 spike (S) protein four ATP. exhibited high sensitivity S ATP detection. Integration with lateral flow assay enabled sensitive within 1 h, revealing its excellent potential portable analysis.

Язык: Английский

Процитировано

10

PAIT effect: Padlock activator inhibits the trans-cleavage activity of CRISPR/Cas12a DOI

Xueying Lei,

Lihua Ding,

Xiaonan Yang

и другие.

Biosensors and Bioelectronics, Год журнала: 2024, Номер 263, С. 116607 - 116607

Опубликована: Июль 26, 2024

Язык: Английский

Процитировано

3

B Doped Zn Single‐Atom Nanozyme With Enhanced Oxidase‐Like Activity Combined CRISPR/Cas13a System for RNA Sensing DOI Open Access
Shuofeng Li, Fangfang Wang, Chenhuan Wang

и другие.

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Янв. 26, 2025

Abstract Single‐atom nanozyme (SAZ) with peroxidase‐like activity has attracted great attention in point‐of‐care (POC) diagnostic, but the use of unstable H 2 O peroxidase catalytic reactions often leads to low detection accuracy. Thus, developing SAZ high oxidase (OD)‐like construct RNA sensing systems independent is imperative for improving Herein, a novel strategy fabricate boron‐nitrogen co‐doped zinc (ZnBNC‐SAZ) excellent OD‐like by carbonizing Zn based zeolitic boron imidazolate frameworks reported. The electron deficient B ligand can form Zn─N─B bond situ during temperature pyrolysis, which regulate electronic structure and further upshift d‐band center improve activity. With ZnBNC‐SAZ as signal generator, new method sensitive developed combining CRISPR/ cas13 system terminal deoxynucleotidyl transferase‐induced DNA extension reaction. limits RNAs are 20 aM. proposed biosensor adaptable lateral‐flow‐based readout universally applicable various programming guide RNA. Importantly, monitor cellular differentiation identify patients cervical carcinoma, showing potential application facile POC diagnosis.

Язык: Английский

Процитировано

0

Fluorescent/colorimetric dual-mode for detecting of MC-LR using bidirectional RCA coupled with CdTe QDs DOI
Weiqing Sun, Xinru Ren, Yilin Xiao

и другие.

Microchimica Acta, Год журнала: 2025, Номер 192(3)

Опубликована: Фев. 25, 2025

Язык: Английский

Процитировано

0

Recent advancements in aptamers as promising nanotool for therapeutic and diagnostic applications DOI
Omar Awad Alsaidan

Analytical Biochemistry, Год журнала: 2025, Номер unknown, С. 115844 - 115844

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

A self-actuated CRISPR/Cas12a feedback amplification platform for ultrasensitive detection of exosome DOI

Meili Yang,

Huan Pan,

Pang Qiao

и другие.

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113477 - 113477

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Rapid detection of Staphylococcus aureus using a CRISPR/Cas14a-assisted electrochemical aptasensor and PCN-222@AuPt nanozyme-induced amplification strategy DOI
Yuanyuan Hui, Xiaoxia Chen, Lusha Wei

и другие.

Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137751 - 137751

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

Bimetallic MOF-derived colorimetric CRISPR/Cas-aptasensor for the detection of sulfadimethoxine utilizing reversible change of peroxidase-mimicking activity of ZrFe MOF nanozymes DOI

Masoomeh Esmaelpourfarkhani,

Pouya Baratian,

Mohammad Ramezani

и другие.

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113580 - 113580

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0

CRISPR/Cas and Argonaute-Based Biosensors for Nucleic acid Detection DOI
Qihua Xiong,

Cancan Zhu,

Xueer Yin

и другие.

Talanta, Год журнала: 2025, Номер 294, С. 128210 - 128210

Опубликована: Апрель 22, 2025

Язык: Английский

Процитировано

0

CRISPR/Cas12a-based fluorescence aptasensor integrated with two-dimensional cobalt oxyhydroxide nanosheets for IFN-γ detection DOI
Rende Mu,

Qiutong Chen,

Xinyi Xia

и другие.

Analytica Chimica Acta, Год журнала: 2023, Номер 1278, С. 341750 - 341750

Опубликована: Авг. 23, 2023

Язык: Английский

Процитировано

8