Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 26, 2025
We
present
a
novel
tool
for
nondestructive
in
vivo
metabolite
analysis,
which
we
call
MetaTube.
This
method
utilizes
high
voltage
to
facilitate
efficient
ionization
while
maintaining
electrical
isolation
from
living
tissues
that
are
sampled.
MetaTube
achieves
2-3
order
of
magnitude
improvement
signal
intensity,
giving
the
capacity
identify
10-fold
more
metabolites.
These
capabilities
allow
us
profile
metabolites
human
skin
surfaces
and
monitor
fluctuations
growing
bacterial
colonies.
offers
promising
strategy
understand
metabolic
dynamics
evaluate
state
real
time.
Acta Pharmaceutica Sinica B,
Год журнала:
2023,
Номер
13(8), С. 3238 - 3251
Опубликована: Май 23, 2023
Emerging
evidence
has
demonstrated
the
vital
role
of
metabolism
in
various
diseases
or
disorders.
Metabolomics
provides
a
comprehensive
understanding
biological
systems.
With
advanced
analytical
techniques,
metabolomics
exhibits
unprecedented
significant
value
basic
drug
research,
including
disease
mechanisms,
identifying
targets,
and
elucidating
mode
action
drugs.
More
importantly,
greatly
accelerates
development
process
by
predicting
pharmacokinetics,
pharmacodynamics,
response.
In
addition,
facilitates
exploration
repurposing
drug-drug
interactions,
as
well
personalized
treatment
strategies.
Here,
we
briefly
review
recent
advances
technologies
update
our
knowledge
applications
research
development.
Journal of the American Society for Mass Spectrometry,
Год журнала:
2023,
Номер
34(8), С. 1798 - 1804
Опубликована: Июль 18, 2023
Untargeted
separation
of
isomeric
and
isobaric
species
in
mass
spectrometry
imaging
(MSI)
is
challenging.
The
combination
ion
mobility
(IMS)
with
MSI
has
emerged
as
an
effective
strategy
for
differentiating
species,
which
substantially
enhances
the
molecular
coverage
specificity
experiments.
In
this
study,
we
have
implemented
nanospray
desorption
electrospray
ionization
(nano-DESI)
on
a
trapped
(TIMS)
spectrometer.
A
new
nano-DESI
source
was
constructed,
specially
designed
inlet
extension
fabricated
to
accommodate
source.
nano-DESI-TIMS-MSI
platform
evaluated
by
mouse
brain
tissue
sections.
We
achieved
high
resolution
utilizing
three
narrow
scan
windows
that
covered
majority
lipid
molecules.
Notably,
reaching
up
300
study
much
higher
than
obtained
our
previous
using
drift
tube
IMS.
High-resolution
TIMS
successfully
separated
isomers
isobars,
revealing
their
distinct
localizations
samples.
Our
results
further
demonstrate
power
high-mobility-resolution
IMS
unraveling
complexity
biomolecular
mixtures
analyzed
The Plant Journal,
Год журнала:
2024,
Номер
119(5), С. 2168 - 2180
Опубликована: Июль 11, 2024
SUMMARY
Mass
spectrometry
imaging
(MSI)
has
become
increasingly
popular
in
plant
science
due
to
its
ability
characterize
complex
chemical,
spatial,
and
temporal
aspects
of
metabolism.
Over
the
past
decade,
as
emerging
unique
features
various
MSI
techniques
have
continued
support
new
discoveries
studies
metabolism
closely
associated
with
function
physiology,
spatial
metabolomics
based
on
positioned
it
at
forefront
metabolic
studies,
providing
opportunity
for
far
higher
resolution
than
was
previously
available.
Despite
these
efforts,
profound
challenges
levels
resolution,
sensitivity,
quantitative
ability,
chemical
confidence,
isomer
discrimination,
multi‐omics
integration,
undoubtedly
remain.
In
this
Perspective,
we
provide
a
contemporary
overview
emergent
widely
used
sciences,
particular
emphasis
recent
advances
methodological
breakthroughs.
Having
established
detailed
context
MSI,
outline
both
golden
opportunities
key
currently
facing
metabolomics,
presenting
our
vision
how
enormous
potential
technologies
will
contribute
progress
coming
years.
Environment International,
Год журнала:
2024,
Номер
186, С. 108620 - 108620
Опубликована: Апрель 1, 2024
Per-
and
polyfluoroalkyl
substances
(PFAS),
ubiquitous
environmental
contaminants,
pose
significant
challenges
to
ecosystems
human
health.
While
cell
cultures
have
emerged
as
new
approach
methodologies
(NAMs)
in
ecotoxicity
research,
metabolomics
is
an
emerging
technique
used
characterize
the
small-molecule
metabolites
present
cells
understand
their
role
various
biological
processes.
Integration
of
with
cultures,
known
culture
metabolomics,
provides
a
novel
robust
tool
unravel
complex
molecular
responses
induced
by
PFAS
exposure.
In
vitro
testing
also
reduces
reliance
on
animal
testing,
aligning
ethical
regulatory
imperatives.
The
current
review
summarizes
key
findings
from
recent
studies
utilizing
investigate
toxicity,
highlighting
alterations
metabolic
pathways,
biomarker
identification,
potential
linkages
between
perturbations.
Additionally,
paper
discusses
different
types
methods
for
contaminants
particularly
PFAS.
Future
perspectives
combination
other
advanced
technologies,
such
single-cell
(SCM),
imaging
mass
spectrometry
(IMS),
extracellular
flux
analysis
(EFA),
multi-omics
are
explored,
which
offers
holistic
understanding
contaminants.
synthesis
knowledge
identification
research
gaps
provide
foundation
future
investigations
that
aim
elucidate
complexities
PFAS-induced
cellular
contribute
development
effective
strategies
mitigating
adverse
effects
Comprehensive Reviews in Food Science and Food Safety,
Год журнала:
2024,
Номер
23(4)
Опубликована: Июнь 25, 2024
Abstract
Food
science
encounters
increasing
complexity
and
challenges,
necessitating
more
efficient,
accurate,
sensitive
analytical
techniques.
Mass
spectrometry
imaging
(MSI)
emerges
as
a
revolutionary
tool,
offering
molecular‐level
insights.
This
review
delves
into
MSI's
applications
challenges
in
food
science.
It
introduces
MSI
principles
instruments
such
matrix‐assisted
laser
desorption/ionization,
desorption
electrospray
ionization,
secondary
ion
mass
spectrometry,
ablation
inductively
coupled
plasma
highlighting
their
application
chemical
composition
analysis,
variety
identification,
authenticity
assessment,
endogenous
substance,
exogenous
contaminant
residue
quality
control,
process
monitoring
processing
storage.
Despite
its
potential,
faces
hurdles
the
cost
of
instrumentation,
sample
preparation,
limited
capabilities,
lack
standardization
for
samples.
While
has
wide
range
analysis
can
provide
comprehensive
accurate
results,
persist,
demanding
further
research
solutions.
The
future
development
directions
include
miniaturization
devices,
high‐resolution
high‐speed
MSI,
multiomics
multimodal
data
fusion,
well
artificial
intelligence.
These
findings
conclusions
valuable
references
insights
field
offer
theoretical
methodological
support
practice
Analytical Science Advances,
Год журнала:
2024,
Номер
5(5-6)
Опубликована: Июнь 1, 2024
Abstract
The
field
of
metabolomics
has
gained
tremendous
interest
in
recent
years.
Whether
the
goal
is
to
discover
biomarkers
related
certain
pathologies
or
better
understand
impact
a
drug
contaminant,
numerous
studies
have
demonstrated
how
crucial
it
variations
metabolism.
Detailed
knowledge
metabolic
variabilities
can
lead
more
effective
treatments,
as
well
faster
less
invasive
diagnostics.
Exploratory
approaches
are
often
employed
metabolomics,
using
relative
quantitation
look
at
perturbations
between
groups
samples.
Most
been
based
on
metabolite
profiling
quantitation,
with
very
few
an
approach
for
absolute
quantitation.
Using
accurate
facilitates
comparison
different
studies,
enabling
longitudinal
studies.
In
this
review,
we
discuss
most
widely
used
techniques
quantitative
mass
spectrometry
(MS).
Various
aspects
will
be
addressed,
such
use
external
and/or
internal
standards,
derivatization
techniques,
vivo
isotopic
labelling,
MS
imaging.
principles,
associated
limitations
and
challenges,
described
each
approach.
