The
capacity
to
produce
complicated
structures
with
high
precision
has
made
it
possible
make
personalized
implants
that
fit
the
individual
anatomy
of
patient,
improving
outcomes
and
lowering
risks.
For
drug
testing
screening,
3D-printed
tissue
models
have
been
employed,
current
research
is
looking
into
how
fabricate
intricate
microstructures
delivery
systems.
This
chapter
explores
3D
printing
potential
transform
healthcare
by
making
create
patient-specific
as
well
useful
tissues
organs
for
transplantation.
It
important
consider
aspects
like
biocompatibility,
printability,
mechanical
qualities,
degradability,
cost
when
choosing
bioinks
biomaterials.
In
vitro
crucial
determine
these
materials'
effectiveness,
used
in
morphological,
rheological,
chemical,
characterization
materials.
Finally,
recent
studies
on
biomaterials
using
various
techniques
are
reviewed,
including
their
limitations
implications
future
research.
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
13(21)
Опубликована: Янв. 22, 2024
Preclinical
models
are
essential
research
tools
before
novel
therapeutic
or
diagnostic
methods
can
be
applied
to
humans.
These
range
from
in
vitro
cell
monocultures
vastly
more
complex
animal
models,
but
clinical
translation
humans
often
fails
deliver
significant
results.
Three-dimensional
(3D)
organoid
systems
being
increasingly
studied
establish
physiologically
relevant
platforms
a
trade-off
between
the
complexity
of
question
and
practical
experimental
setups.
The
sensitivity
precision
analytical
yet
another
limiting
factors
what
investigated,
mass
spectrometry
(MS)
is
one
most
powerful
techniques
available
scientific
community.
Its
innovative
use
spatially
resolve
biological
samples
has
opened
many
avenues
field
MS
imaging
(MSI).
Here,
this
work
aims
explore
current
landscape
application
MSI
on
organoids,
with
an
emphasis
their
combined
potential
facilitate
improve
preclinical
studies.
Heliyon,
Год журнала:
2023,
Номер
10(1), С. e23504 - e23504
Опубликована: Дек. 9, 2023
With
the
burgeoning
advancements
in
disease
modeling,
drug
development,
and
precision
medicine,
organ-on-a-chip
has
risen
to
forefront
of
biomedical
research.
Specifically
tumor
research,
this
technology
exhibited
exceptional
potential
elucidating
dynamics
metastasis
within
microenvironment.
Recognizing
significance
field,
our
study
aims
provide
a
comprehensive
bibliometric
analysis
global
scientific
contributions
related
organ-on-a-chip.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Окт. 2, 2024
Our
previous
article
entitled
"Proteomics
and
its
applications
in
breast
cancer",
proposed
a
Breast
Cancer
Continuum
Concept
(BCCC),
including
Cell
as
well
Proteomic
Concept.
cancer-on-chip
(BCoC),
cancer
liquid
biopsy-on-chip
(BCLBoC),
metastasis-on-chip
(BCMoC)
models
successfully
recapitulate
reproduce
Understanding
the
intricate
mechanisms
underlying
brain-related
diseases
hinges
on
unraveling
pivotal
role
of
blood-brain
barrier
(BBB),
an
essential
dynamic
interface
crucial
for
maintaining
brain
equilibrium.
This
review
offers
a
comprehensive
analysis
BBB
physiology,
delving
into
its
cellular
and
molecular
components
while
exploring
wide
range
in
vivo
vitro
models.
Notably,
recent
advancements
3D
cell
culture
techniques
are
explicitly
discussed,
as
they
have
significantly
improved
fidelity
modeling
by
enabling
replication
physiologically
relevant
environments
under
flow
conditions.
Special
attention
is
given
to
aspects
models,
alongside
discussions
advances
stem
technologies,
providing
valuable
insights
generating
robust
systems
modeling.
The
diverse
array
types
used
modeling,
depending
their
sources,
meticulously
examined
this
review,
scrutinizing
respective
derivation
protocols
implications.
By
synthesizing
approaches,
sheds
light
improvements
models
capture
physiological
conditions,
aiding
understanding
interactions
health
disease
conditions
foster
clinical
developments.
Journal of Separation Science,
Год журнала:
2023,
Номер
46(18)
Опубликована: Авг. 1, 2023
Ultralow
flow
LC
employs
ultra‐narrow
bore
columns
and
mid‐range
pL/min
to
low
nL/min
rates
(i.e.,
≤20
nL/min).
The
separation
that
are
used
under
these
conditions
typically
2–30
μm
in
inner
diameter.
systems
allow
for
exceptionally
high
sensitivity
frequently
resolution.
There
has
been
an
increasing
interest
the
analysis
of
scarce
biological
samples,
example,
circulating
tumor
cells,
extracellular
vesicles,
organelles,
single
ultralow
was
efficiently
applied
such
samples.
Hence,
advances
towards
dedicated
instrumentation,
technical
approaches,
higher
throughput
(e.g.,
tens‐to‐hundreds
cells
analyzed
per
day)
were
recently
made.
Here,
we
review
types
technology,
followed
by
a
discussion
selected
representative
applications,
focusing
on
progress
made
bioanalysis
amount‐limited
samples
during
last
10
years.
We
also
discuss
several
reported
high‐sensitivity
applications
utilizing
up
100
nL/min,
which
below
commonly
nanoLC
rates.
Finally,
path
forward
future
developments
LC.
LCGC Europe,
Год журнала:
2023,
Номер
unknown, С. 16 - 19
Опубликована: Май 2, 2023
Organoids
are
laboratory-grown
three-dimensional
(3D)
models
of
organs
and
emerging
tools
for
studies
into
developmental
biology,
drug
discovery,
toxicology.
complex
biological
materials,
proteomics
organoids
can
benefit
from
applying
high-resolution
chromatography
devices
before
mass
spectrometric
analysis.
Micro-pillar
array
columns
have
been
shown
to
provide
excellent
resolution
peptide
products
proteolytic
digestion
proteins
bottom-up
proteomics.This
article
describes
a
workflow
incorporating
micro-pillar
column
mapping
the
proteome
human
stem
cell-derived
liver
(sample
preparation
using
SPEED
protocol)
trapped
ion
mobility
time-of-flight
spectrometry
(timsTOF-MS).
International Journal of Health Sciences,
Год журнала:
2024,
Номер
8(S1), С. 972 - 998
Опубликована: Авг. 5, 2024
Organoids
are
three-dimensional
(3D)
cell
culture
systems
derived
from
human
pluripotent
stem
cells
or
organotypic
differentiation,
replicating
the
complex
interactions
and
functionalities
of
actual
organs.
These
offer
significant
advantages
for
studying
tissue
organ
biology,
addressing
limitations
animal
models
related
to
sample
accessibility
ethical
concerns.
Liver
organoids,
in
particular,
advanced
developed
study
hepatic
phenotypes,
encompassing
various
types
enabling
detailed
investigation
cellular,
molecular,
genetic
aspects
liver
diseases,
drug
metabolism,
protein
secretion.
They
hold
promise
fundamental
research,
discovery,
regenerative
medicine
applications.
Despite
their
potential,
organoids
face
such
as
simplicity,
lack
high-fidelity
types,
flexibility,
atypical
physiology.
Enhancements
liver-like
surrogates,
incorporating
vivo-like
architecture,
along
with
advancements
microfluidic
chip
technology,
expected
improve
disease,
toxicity,
paving
way
new
treatments.
This
review
will
provide
an
overview
history
development
current
progress,
challenges,
applications,
future
prospects
field
personalized
medicine.
