Recent advances in molecular and nanoparticle probes for fluorescent bioanalysis
Nano Research,
Год журнала:
2024,
Номер
17(7), С. 6443 - 6474
Опубликована: Апрель 12, 2024
Язык: Английский
Sample‐to‐answer salivary miRNA testing: New frontiers in point‐of‐care diagnostic technologies
Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology,
Год журнала:
2024,
Номер
16(3)
Опубликована: Май 1, 2024
Abstract
MicroRNA
(miRNA),
crucial
non‐coding
RNAs,
have
emerged
as
key
biomarkers
in
molecular
diagnostics,
prognosis,
and
personalized
medicine
due
to
their
significant
role
gene
expression
regulation.
Salivary
miRNA,
particular,
stands
out
for
its
non‐invasive
collection
method
ease
of
accessibility,
offering
promising
avenues
the
development
point‐of‐care
diagnostics
a
spectrum
diseases,
including
cancer,
neurodegenerative
disorders,
infectious
diseases.
Such
promises
rapid
precise
diagnosis,
enabling
timely
treatment.
Despite
advancements
salivary
miRNA‐based
testing,
challenges
persist
quantification,
multiplexing,
sensitivity,
specificity,
particularly
miRNA
at
low
concentrations
complex
biological
mixtures.
This
work
delves
into
these
challenges,
focusing
on
application
tests
use.
We
explore
biogenesis
analyze
quantitative
disease
relevance
infection,
disorders.
also
examined
recent
progress
extraction,
amplification,
multiplexed
detection
methods.
study
offers
comprehensive
view
testing
(POCT).
Its
successful
advancement
could
revolutionize
early
detection,
monitoring,
management
various
conditions,
enhancing
healthcare
outcomes.
article
is
categorized
under:
Diagnostic
Tools
>
Biosensing
Nanodevices
Язык: Английский
Advance in intracellular telomerase imaging for cancer diagnosis and therapy
Coordination Chemistry Reviews,
Год журнала:
2024,
Номер
509, С. 215801 - 215801
Опубликована: Март 23, 2024
Язык: Английский
Enzyme-Free Nano-Sensor for High-Sensitivity miRNA Detection and Tumor Imaging Based on Multicolor Silver Nanoclusters Pair and DNA-Mediated CHA Reactions
Sensors and Actuators B Chemical,
Год журнала:
2025,
Номер
427, С. 137222 - 137222
Опубликована: Янв. 5, 2025
Язык: Английский
Deep Learning-Assisted Fluorescence Single-Particle Detection of Fumonisin B1 Powered by Entropy-Driven Catalysis and Argonaute
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 27, 2025
Timely
and
accurate
detection
of
trace
mycotoxins
in
agricultural
products
food
is
significant
for
ensuring
safety
public
health.
Herein,
a
deep
learning-assisted
entropy-driven
catalysis
(EDC)-Argonaute
powered
fluorescence
single-particle
aptasensing
platform
was
developed
ultrasensitive
fumonisin
B1
(FB1)
using
single-stranded
DNA
modified
with
biotin
red
fluorescence-encoded
microspheres
as
signal
probe
streptavidin-conjugated
magnetic
beads
separation
carriers.
The
binding
aptamer
FB1
releases
the
trigger
sequence
to
mediate
EDC
cycle
produce
numerous
5′-phosphorylated
output
sequences,
which
can
be
used
guide
activate
downstream
Thermus
thermophilus
Argonaute
(TtAgo)
cleaving
probe,
resulting
increased
number
remaining
final
reaction
supernatant
after
separation.
Subsequently,
through
fast
counting
bright
particles
captured
confocal
images
from
via
YOLOv9
learning
model,
sensitive
specific
could
realized.
This
approach
has
limit
(LOD)
0.89
pg/mL
linear
range
1
100
ng/mL,
satisfactory
recovery
(87.2–113.5%)
real
samples
indicates
its
practicality.
integration
TtAgo
broadens
target
enhances
sensitivity.
Furthermore,
incorporating
significantly
improves
analytical
efficiency
detection.
work
provides
promising
strategy
biosensing
promotes
application
monitoring.
Язык: Английский
Plasmonic Biosensors in Cancer-Associated miRNA Detection
Biosensors,
Год журнала:
2025,
Номер
15(3), С. 165 - 165
Опубликована: Март 4, 2025
Cancer
is
one
of
the
most
lethal
diseases
and
has
distinct
variants
that
affect
over
60
organs
in
human
body.
The
necessity
advanced
methodologies
for
early
diagnosis
cancer
grown
past
decades.
Among
various
biomarkers,
microRNAs
(miRNAs)
have
emerged
as
highly
specific
minimally
invasive
indicators
detection,
prognosis,
treatment
monitoring.
Their
stability
biological
fluids
their
critical
role
gene
regulation
make
them
valuable
targets
diagnostic
applications.
Plasmonic
biosensors
gained
massive
attention
owing
to
unique
optical
properties,
such
surface
plasmon
resonance,
making
promising
tools
sensitive
selective
analysis
cancer-associated
biomarkers.
In
contrast
previous
reviews,
this
work
offers
a
comprehensive
overview
advancements
from
approximately
five
years,
particularly
detection
miRNAs.
It
emphasizes
emerging
plasmonic
sensing
strategies,
integration
with
novel
nanomaterials,
enhanced
signal
amplification
techniques.
By
focusing
on
these
recent
innovations,
review
provides
new
insights
into
potential
improve
treatment.
Язык: Английский
Recent advance in optical single-molecule detection of methylation modification and methyl-modifying enzymes
TrAC Trends in Analytical Chemistry,
Год журнала:
2024,
Номер
172, С. 117553 - 117553
Опубликована: Янв. 23, 2024
Язык: Английский
Mix-and-Read Digital MicroRNA Analysis Based on Flow Cytometric Counting of Target-Clicked Nanobead Dimer
Yan Qi,
Junyue Sun,
Shan Wang
и другие.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(33), С. 13734 - 13741
Опубликована: Авг. 8, 2024
A
one-step,
enzyme-free,
and
highly
sensitive
digital
microRNA
(miRNA)
assay
is
rationally
devised
based
on
flow
cytometric
counting
of
target
miRNA-clicked
nanobead
dimers
via
a
facile
mix-and-read
manner.
In
this
strategy,
efficient
miRNA-sandwiched
click
chemical
ligation
two
DNA
probes
may
remarkably
stabilize
boost
the
dimer
formation
between
kinds
fluorescence-coded
nanobeads,
number
as-produced
bead
will
be
dose-responsive,
particularly
when
trace
miRNA
much
less
than
that
employed
nanobeads.
Finally,
each
can
easily
identified
digitally
counted
by
powerful
cytometer
(FCM)
accordingly,
amount
accurately
quantified
in
way.
This
new
accomplished
with
operation
just
simply
mixing
preprepared
probe-functionalized
which
do
not
require
any
nucleic
acid
amplification,
purification,
complex
procedures.
spite
extremely
simple
one-step
operation,
benefiting
from
low-background
but
high
target-mediated
efficiency,
powerfully
statistical
capability
FCM,
strategy
achieves
sensitivity
quite
low
detection
limit
5.2
fM
as
well
specificity
good
generality
for
analysis,
pioneering
direction
fabricating
bioassays.
Язык: Английский
Quantitative Analysis of Cell-Free RNA at Attomolar Level Using CRISPR/Cas Digital Imaging Platform
Y Li,
Fenglei Quan,
Yige Wu
и другие.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(43), С. 17362 - 17369
Опубликована: Окт. 16, 2024
Quantitative
analysis
of
cell-free
RNA
(cfRNA)
in
plasma
sample
can
be
used
for
screening,
diagnosing,
and
prognosticating
multiple
diseases.
Here,
we
report
a
quantitative
CRISPR/Cas
digital
imaging
platform
(qCasdip)
the
detection
various
cfRNAs,
including
circular
RNAs
miRNAs,
clinical
samples
at
attomolar
(aM)
level
without
need
preamplification.
Digital
counting
strategy
provides
qCasdip
ability
with
linear
range
10
Язык: Английский