Recent advances in molecular and nanoparticle probes for fluorescent bioanalysis

Nano Research, Journal Year: 2024, Volume and Issue: 17(7), P. 6443 - 6474

Published: April 12, 2024

Language: Английский

---

Sample‐to‐answer salivary miRNA testing: New frontiers in point‐of‐care diagnostic technologies

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Journal Year: 2024, Volume and Issue: 16(3)

Published: May 1, 2024

Abstract MicroRNA (miRNA), crucial non‐coding RNAs, have emerged as key biomarkers in molecular diagnostics, prognosis, and personalized medicine due to their significant role gene expression regulation. Salivary miRNA, particular, stands out for its non‐invasive collection method ease of accessibility, offering promising avenues the development point‐of‐care diagnostics a spectrum diseases, including cancer, neurodegenerative disorders, infectious diseases. Such promises rapid precise diagnosis, enabling timely treatment. Despite advancements salivary miRNA‐based testing, challenges persist quantification, multiplexing, sensitivity, specificity, particularly miRNA at low concentrations complex biological mixtures. This work delves into these challenges, focusing on application tests use. We explore biogenesis analyze quantitative disease relevance infection, disorders. also examined recent progress extraction, amplification, multiplexed detection methods. study offers comprehensive view testing (POCT). Its successful advancement could revolutionize early detection, monitoring, management various conditions, enhancing healthcare outcomes. article is categorized under: Diagnostic Tools > Biosensing Nanodevices

Language: Английский

---

Advance in intracellular telomerase imaging for cancer diagnosis and therapy

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 509, P. 215801 - 215801

Published: March 23, 2024

Language: Английский

---

Enzyme-Free Nano-Sensor for High-Sensitivity miRNA Detection and Tumor Imaging Based on Multicolor Silver Nanoclusters Pair and DNA-Mediated CHA Reactions

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 427, P. 137222 - 137222

Published: Jan. 5, 2025

Language: Английский

---

Deep Learning-Assisted Fluorescence Single-Particle Detection of Fumonisin B₁ Powered by Entropy-Driven Catalysis and Argonaute

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Timely and accurate detection of trace mycotoxins in agricultural products food is significant for ensuring safety public health. Herein, a deep learning-assisted entropy-driven catalysis (EDC)-Argonaute powered fluorescence single-particle aptasensing platform was developed ultrasensitive fumonisin B1 (FB1) using single-stranded DNA modified with biotin red fluorescence-encoded microspheres as signal probe streptavidin-conjugated magnetic beads separation carriers. The binding aptamer FB1 releases the trigger sequence to mediate EDC cycle produce numerous 5′-phosphorylated output sequences, which can be used guide activate downstream Thermus thermophilus Argonaute (TtAgo) cleaving probe, resulting increased number remaining final reaction supernatant after separation. Subsequently, through fast counting bright particles captured confocal images from via YOLOv9 learning model, sensitive specific could realized. This approach has limit (LOD) 0.89 pg/mL linear range 1 100 ng/mL, satisfactory recovery (87.2–113.5%) real samples indicates its practicality. integration TtAgo broadens target enhances sensitivity. Furthermore, incorporating significantly improves analytical efficiency detection. work provides promising strategy biosensing promotes application monitoring.

Language: Английский

---

Plasmonic Biosensors in Cancer-Associated miRNA Detection

Biosensors, Journal Year: 2025, Volume and Issue: 15(3), P. 165 - 165

Published: March 4, 2025

Cancer is one of the most lethal diseases and has distinct variants that affect over 60 organs in human body. The necessity advanced methodologies for early diagnosis cancer grown past decades. Among various biomarkers, microRNAs (miRNAs) have emerged as highly specific minimally invasive indicators detection, prognosis, treatment monitoring. Their stability biological fluids their critical role gene regulation make them valuable targets diagnostic applications. Plasmonic biosensors gained massive attention owing to unique optical properties, such surface plasmon resonance, making promising tools sensitive selective analysis cancer-associated biomarkers. In contrast previous reviews, this work offers a comprehensive overview advancements from approximately five years, particularly detection miRNAs. It emphasizes emerging plasmonic sensing strategies, integration with novel nanomaterials, enhanced signal amplification techniques. By focusing on these recent innovations, review provides new insights into potential improve treatment.

Language: Английский

---

Recent advance in optical single-molecule detection of methylation modification and methyl-modifying enzymes

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 172, P. 117553 - 117553

Published: Jan. 23, 2024

Language: Английский

---

Mix-and-Read Digital MicroRNA Analysis Based on Flow Cytometric Counting of Target-Clicked Nanobead Dimer

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(33), P. 13734 - 13741

Published: Aug. 8, 2024

A one-step, enzyme-free, and highly sensitive digital microRNA (miRNA) assay is rationally devised based on flow cytometric counting of target miRNA-clicked nanobead dimers via a facile mix-and-read manner. In this strategy, efficient miRNA-sandwiched click chemical ligation two DNA probes may remarkably stabilize boost the dimer formation between kinds fluorescence-coded nanobeads, number as-produced bead will be dose-responsive, particularly when trace miRNA much less than that employed nanobeads. Finally, each can easily identified digitally counted by powerful cytometer (FCM) accordingly, amount accurately quantified in way. This new accomplished with operation just simply mixing preprepared probe-functionalized which do not require any nucleic acid amplification, purification, complex procedures. spite extremely simple one-step operation, benefiting from low-background but high target-mediated efficiency, powerfully statistical capability FCM, strategy achieves sensitivity quite low detection limit 5.2 fM as well specificity good generality for analysis, pioneering direction fabricating bioassays.

Language: Английский

---

Quantitative Analysis of Cell-Free RNA at Attomolar Level Using CRISPR/Cas Digital Imaging Platform

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(43), P. 17362 - 17369

Published: Oct. 16, 2024

Quantitative analysis of cell-free RNA (cfRNA) in plasma sample can be used for screening, diagnosing, and prognosticating multiple diseases. Here, we report a quantitative CRISPR/Cas digital imaging platform (qCasdip) the detection various cfRNAs, including circular RNAs miRNAs, clinical samples at attomolar (aM) level without need preamplification. Digital counting strategy provides qCasdip ability with linear range 10

Language: Английский

---