Green metrics for analytical methodologies: Uncovering sustainable chromatography approaches for detecting emerging contaminants in food and environmental water

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 172, С. 117598 - 117598

Опубликована: Фев. 16, 2024

Язык: Английский

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Computer-assisted multifactorial method development for the streamlined separation and analysis of multicomponent mixtures in (Bio)pharmaceutical settings

Analytica Chimica Acta, Год журнала: 2023, Номер 1293, С. 342178 - 342178

Опубликована: Дек. 27, 2023

Язык: Английский

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Applications of Hydrophilic Interaction and Mixed-mode Liquid Chromatography in Pharmaceutical Analysis

Journal of Chromatography A, Год журнала: 2024, Номер 1739, С. 465524 - 465524

Опубликована: Ноя. 14, 2024

Язык: Английский

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A validated single-step saliva and serum sample extraction LC-MS/MS method for the analysis of nicotine, cotinine and 3’-hydroxycotinine for clinical vaping studies

Journal of Pharmaceutical and Biomedical Analysis, Год журнала: 2025, Номер unknown, С. 116703 - 116703

Опубликована: Янв. 1, 2025

Язык: Английский

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Getting Insights Into Chromatographic Properties of HILIС and Mixed‐Mode Homemade Stationary Phases Using Principal Component and Cluster Analyses

Journal of Chemometrics, Год журнала: 2025, Номер 39(3)

Опубликована: Март 1, 2025

ABSTRACT In this work, we compared the chromatographic properties of 27 homemade monomer‐ and polymer‐modified stationary phases synthesized via Ugi reaction for hydrophilic interaction liquid chromatography (HILIC). These along with unmodified substrate were characterized by retention factors 33 polar biologically active compounds belonging to various classes (nucleobases/nucleosides, sugars, carboxylic acids, water‐soluble vitamins). Additionally, widely used Tanaka HILIC test was performed. The experimental data from both characterization approaches processed using several chemometric techniques, including principal component analysis (PCA), hierarchical cluster (HCA), K‐means algorithm. It initially expected that would differ significantly monomer‐modified ones due their mixed‐mode properties. confirmed clear separation these two types on PCA score plot obtained binary logarithms selectivities (calculated all factors). Dissimilarities observed among some resulted in insights into conditions suitable obtaining adsorbents distinct Each class required specific mobile phase composition achieve reasonable characteristics, such as times peak shapes. To exclude long‐lasting re‐equilibration stage associated changes, a smaller set only three proposed, yielding nearly same clustering results complete dataset. This simplified procedure can facilitate rapid newly allow comparison previously studied phases.

Язык: Английский

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Impact of Mobile Phase Composition on Separation Selectivity of Labeled Dextran Ladder in Hydrophilic Interaction Liquid Chromatography

Molecules, Год журнала: 2025, Номер 30(6), С. 1327 - 1327

Опубликована: Март 15, 2025

The glycosylation process plays a crucial role in the structural integrity and biological activity of glycoproteins, where glycans are attached to protein backbone. There many kinds glycans, most common being N-glycans, which can be arranged into three classes, that is, complex, hybrid, high mannoses, forming structurally very diverse set polar compounds difficult detect separate. Most commonly, N-glycans labeled before separation by charged or fluorescence tags for better MS detection, respectively. This study examines influence ionic strength organic modifier selection on fluorescently dextran ladders Hydrophilic Interaction Liquid Chromatography (HILIC). Using Glycan BEH Amide column varying ammonium formate buffer concentration along with acetonitrile methanol ratios, we investigated analyte retention, efficiency, post-column conductivity changes. Our findings reveal changes mobile phase do not contribute selectivity, neither when nor were used as modifiers phase. However, addition significantly mechanism two different prevailing separations mechanisms identified. It was assumed influences folding pattern dextrans around permanent positive charge added tag, selectivity. work presents systematic approach altering composition (buffer concentration, type) control retention selectivity complex glycan analysis. discovery alters behavior provides potential new method refining HILIC compounds.

Язык: Английский

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Preparation of a 1,4-Diazabicyclo[2.2.2]octane Sulfonate Betaine Zwitterionic Stationary Phase and Comparative Evaluation of Its Separation Performance in Hydrophilic Interaction Chromatography

Analytica Chimica Acta, Год журнала: 2025, Номер unknown, С. 344028 - 344028

Опубликована: Апрель 1, 2025

Язык: Английский

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Bioinspired hydrophilic material with parallel ordered channels for simultaneously selective enrichment and rapid separation of glycopeptides and phosphopeptides

Talanta, Год журнала: 2025, Номер 295, С. 128199 - 128199

Опубликована: Апрель 25, 2025

Язык: Английский

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Stable cross-linked polyacrylamide stationary phase based on atom transfer radical polymerization for hydrophilic interaction liquid chromatography

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113818 - 113818

Опубликована: Май 1, 2025

Язык: Английский

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Quantification of Epigenetic DNA and RNA Modifications by UHPLC–MS/MS Technologies: New Concepts and New Improvements for the Special Collections

Journal of Separation Science, Год журнала: 2025, Номер 48(5)

Опубликована: Май 1, 2025

ABSTRACT Dynamic and reversible DNA RNA modifications are essential for cell differentiation development. Aberrant epigenetic closely associated with the occurrence progression of diseases, serving as potential markers cancer diagnosis prognosis. Ultra‐high‐performance liquid chromatography coupled tandem mass spectrometry (UHPLC–MS/MS) offers distinct advantages in qualitative quantitative analysis various due to its sensitivity, specificity, accuracy. This review provides a comprehensive overview current knowledge regarding chromatography–mass (LC–MS) modifications, including analytical procedures, advancements, biological applications, focus on tracing source (N6‐2′‐deoxy‐adenosine) 6mdA eukaryotes. Additionally, we examine integration UHPLC–MS/MS other separation techniques achieve accurate quantification specific regions, certain fragments, free nucleosides.

Язык: Английский

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