Comprehensive analysis of the effective and intra-particle diffusion of weakly retained compounds in silica hydrophilic interaction liquid chromatography columns DOI
Lídia Redón, Xavier Subirats, Soraya Chapel

et al.

Journal of Chromatography A, Journal Year: 2023, Volume and Issue: 1713, P. 464529 - 464529

Published: Nov. 23, 2023

Language: Английский

Green metrics for analytical methodologies: Uncovering sustainable chromatography approaches for detecting emerging contaminants in food and environmental water DOI
Nor Munira Hashim, Maisarah Nasution Waras, Noorfatimah Yahaya

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 172, P. 117598 - 117598

Published: Feb. 16, 2024

Language: Английский

Citations

6
Computer-assisted multifactorial method development for the streamlined separation and analysis of multicomponent mixtures in (Bio)pharmaceutical settings DOI
Mohamed Hemida, Imad A. Haidar Ahmad, Rodell C. Barrientos

et al.

Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1293, P. 342178 - 342178

Published: Dec. 27, 2023

Language: Английский

Citations

12
Applications of Hydrophilic Interaction and Mixed-mode Liquid Chromatography in Pharmaceutical Analysis DOI
Bingchuan Wei,

Lulu Dai,

Kelly Zhang

et al.

Journal of Chromatography A, Journal Year: 2024, Volume and Issue: 1739, P. 465524 - 465524

Published: Nov. 14, 2024

Language: Английский

Citations

4
A validated single-step saliva and serum sample extraction LC-MS/MS method for the analysis of nicotine, cotinine and 3’-hydroxycotinine for clinical vaping studies DOI
Vera van der Velpen, Evangelia Liakoni,

M. A. Hirt

et al.

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 116703 - 116703

Published: Jan. 1, 2025

Language: Английский

Citations

0
Getting Insights Into Chromatographic Properties of HILIС and Mixed‐Mode Homemade Stationary Phases Using Principal Component and Cluster Analyses DOI Open Access
A. Shemiakina, Mikhail Khrisanfov,

N. Yu. Chikurova

et al.

Journal of Chemometrics, Journal Year: 2025, Volume and Issue: 39(3)

Published: March 1, 2025

ABSTRACT In this work, we compared the chromatographic properties of 27 homemade monomer‐ and polymer‐modified stationary phases synthesized via Ugi reaction for hydrophilic interaction liquid chromatography (HILIC). These along with unmodified substrate were characterized by retention factors 33 polar biologically active compounds belonging to various classes (nucleobases/nucleosides, sugars, carboxylic acids, water‐soluble vitamins). Additionally, widely used Tanaka HILIC test was performed. The experimental data from both characterization approaches processed using several chemometric techniques, including principal component analysis (PCA), hierarchical cluster (HCA), K‐means algorithm. It initially expected that would differ significantly monomer‐modified ones due their mixed‐mode properties. confirmed clear separation these two types on PCA score plot obtained binary logarithms selectivities (calculated all factors). Dissimilarities observed among some resulted in insights into conditions suitable obtaining adsorbents distinct Each class required specific mobile phase composition achieve reasonable characteristics, such as times peak shapes. To exclude long‐lasting re‐equilibration stage associated changes, a smaller set only three proposed, yielding nearly same clustering results complete dataset. This simplified procedure can facilitate rapid newly allow comparison previously studied phases.

Language: Английский

Citations

0
Impact of Mobile Phase Composition on Separation Selectivity of Labeled Dextran Ladder in Hydrophilic Interaction Liquid Chromatography DOI Creative Commons

Matjaž Grčman,

Niko R. Pompe,

Drago Kočar

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(6), P. 1327 - 1327

Published: March 15, 2025

The glycosylation process plays a crucial role in the structural integrity and biological activity of glycoproteins, where glycans are attached to protein backbone. There many kinds glycans, most common being N-glycans, which can be arranged into three classes, that is, complex, hybrid, high mannoses, forming structurally very diverse set polar compounds difficult detect separate. Most commonly, N-glycans labeled before separation by charged or fluorescence tags for better MS detection, respectively. This study examines influence ionic strength organic modifier selection on fluorescently dextran ladders Hydrophilic Interaction Liquid Chromatography (HILIC). Using Glycan BEH Amide column varying ammonium formate buffer concentration along with acetonitrile methanol ratios, we investigated analyte retention, efficiency, post-column conductivity changes. Our findings reveal changes mobile phase do not contribute selectivity, neither when nor were used as modifiers phase. However, addition significantly mechanism two different prevailing separations mechanisms identified. It was assumed influences folding pattern dextrans around permanent positive charge added tag, selectivity. work presents systematic approach altering composition (buffer concentration, type) control retention selectivity complex glycan analysis. discovery alters behavior provides potential new method refining HILIC compounds.

Language: Английский

Citations

0
Preparation of a 1,4-Diazabicyclo[2.2.2]octane Sulfonate Betaine Zwitterionic Stationary Phase and Comparative Evaluation of Its Separation Performance in Hydrophilic Interaction Chromatography DOI

Yongxing Hu,

Pengcheng Zhang, Ashraf Ali

et al.

Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 344028 - 344028

Published: April 1, 2025

Language: Английский

Citations

0
Bioinspired hydrophilic material with parallel ordered channels for simultaneously selective enrichment and rapid separation of glycopeptides and phosphopeptides DOI

Jie Li,

Junlong Wang, Jiabin Cui

et al.

Talanta, Journal Year: 2025, Volume and Issue: 295, P. 128199 - 128199

Published: April 25, 2025

Language: Английский

Citations

0
Stable cross-linked polyacrylamide stationary phase based on atom transfer radical polymerization for hydrophilic interaction liquid chromatography DOI
Zhipeng Zhu, Yuqian Xiang, Xiaohui Yan

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113818 - 113818

Published: May 1, 2025

Language: Английский

Citations

0
Quantification of Epigenetic DNA and RNA Modifications by UHPLC–MS/MS Technologies: New Concepts and New Improvements for the Special Collections DOI
Rui Zhang, Hailong Liu,

Biao Bai

et al.

Journal of Separation Science, Journal Year: 2025, Volume and Issue: 48(5)

Published: May 1, 2025

ABSTRACT Dynamic and reversible DNA RNA modifications are essential for cell differentiation development. Aberrant epigenetic closely associated with the occurrence progression of diseases, serving as potential markers cancer diagnosis prognosis. Ultra‐high‐performance liquid chromatography coupled tandem mass spectrometry (UHPLC–MS/MS) offers distinct advantages in qualitative quantitative analysis various due to its sensitivity, specificity, accuracy. This review provides a comprehensive overview current knowledge regarding chromatography–mass (LC–MS) modifications, including analytical procedures, advancements, biological applications, focus on tracing source (N6‐2′‐deoxy‐adenosine) 6mdA eukaryotes. Additionally, we examine integration UHPLC–MS/MS other separation techniques achieve accurate quantification specific regions, certain fragments, free nucleosides.

Language: Английский

Citations

0