Visually evaluating drug efficacy in living cells using COF-based fluorescent nanoprobe via CHA amplified detection of miRNA and simultaneous apoptosis imaging

Analytica Chimica Acta, Год журнала: 2024, Номер 1302, С. 342502 - 342502

Опубликована: Март 19, 2024

Язык: Английский

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Cascade-amplification-based electrochemical detection of Akashiwo sanguinea at pre-outbreak stage

Talanta, Год журнала: 2025, Номер 287, С. 127671 - 127671

Опубликована: Фев. 6, 2025

Язык: Английский

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N-Doped Porous Carbon Synergistic Freezing-Induced DNA with Catalyzed Hairpin Assembly Enables Electrochemical One-Pot Detection of Pathogen in Food Samples

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 7, 2025

A DNA electrochemical interface biosensor based on screen-printed carbon electrodes (SPCEs) holds promise for point-of-care testing (POCT) detection of pathogens in food safety. Nevertheless, SPCE commonly has a rough surface and suffers from relatively low electron transfer rate, disorder capture probes (CPs), the steric hindrance effect target nucleic acid binding. These issues lead to sensitivity. Herein, simple rapid N-doped porous (NPC)-modified freezing-directed combined with catalyzed hairpin assembly (CHA) was constructed one-pot samples without time-consuming growth cultures. The by modified NPC enhanced properties, CP designed CHA stably fixed electrode high hybridization efficiency. Moreover, signals amplified enable selective sensitive washing steps. This method is wide linear range 101 107 CFU/mL limit 5 Escherichia coli shows specificity against other coexisting pathogens. whole complex performed only within 60 min sample-to-answer, which great potential POCT

Язык: Английский

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Development of Ziziphus spina-christi (Sidr) leaf extract-loaded hyaluronic acid and their potential in mitigating oxidative stress during semen cryopreservation

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140928 - 140928

Опубликована: Фев. 1, 2025

Язык: Английский

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Silver-loaded porous crystalline frameworks materials: A synergistic strategy for advanced antibacterial applications

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 162049 - 162049

Опубликована: Апрель 1, 2025

Язык: Английский

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A colorimetric and electrochemical dual-modal ochratoxin a aptasensor based on branched hybridization chain reaction signal amplification

Bioelectrochemistry, Год журнала: 2025, Номер unknown, С. 108984 - 108984

Опубликована: Апрель 1, 2025

Язык: Английский

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Tethered Catalytic Hairpin Assembly with Plasmon‐Enhanced Fluorescence Readout for Single Molecule Detection

Small Methods, Год журнала: 2025, Номер unknown

Опубликована: Апрель 10, 2025

Abstract Here a novel digital bioassay readout concept is reported that does not rely on enzymatic amplification nor compartmenting of an analyzed liquid sample. Rather, it based counting individual affinity‐captured target biomolecules via the use tethered catalytic hairpin assembly (tCHA) deployed solid sensor surface with spatial confinement utilized by flexible polymer linker (FPL). Wide‐field plasmon‐enhanced fluorescence (PEF) imaging employed for optical real‐time probing reaction kinetics, where molecules are manifested as spatially distinct bright fluorescent spots. The effect length FPLs investigated, and analytical performance dual tCHA‐PEF tested using model short single‐stranded DNA analyte. When applied in sandwich immunoassay, detection proteins at sub‐femtomolar concentrations demonstrated. experiments supported diffusion‐limited mass transfer models document potential new class generic enzyme‐free bioanalytical tools enabling ultrasensitive analysis trace amounts protein nucleic acid analytes, making attractive future molecular diagnostics research applications.

Язык: Английский

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Multivalent aptamer-linked tetrahedron DNA assisted catalytic hairpin assembly for accurate SERS assay of cancer-derived exosomes in clinical blood

Biosensors and Bioelectronics, Год журнала: 2025, Номер 282, С. 117497 - 117497

Опубликована: Апрель 18, 2025

Язык: Английский

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A label-free colorimetric aptasensor for sensitive detection of aflatoxin B1 in peanuts based on dual catalytic hairpin assembly

Microchemical Journal, Год журнала: 2024, Номер unknown, С. 111948 - 111948

Опубликована: Окт. 1, 2024

Язык: Английский

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Combined Detection of Hepatitis B Virus Surface Antigen and Hepatitis B Virus DNA using DNA sensor

Analytical Methods, Год журнала: 2024, Номер 16(43), С. 7319 - 7324

Опубликована: Янв. 1, 2024

A multi-detection strategy based on branched DNA nanostructures was developed to achieve the simultaneous high specificity identification and highly sensitive fluorescence responses HBV antigen DNA.

Язык: Английский

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