Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: unknown, P. 117090 - 117090
Published: Dec. 1, 2024
Language: Английский
Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: unknown, P. 117090 - 117090
Published: Dec. 1, 2024
Language: Английский
Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1302, P. 342502 - 342502
Published: March 19, 2024
Language: Английский
Citations
6Talanta, Journal Year: 2025, Volume and Issue: 287, P. 127671 - 127671
Published: Feb. 6, 2025
Language: Английский
Citations
0Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 7, 2025
A DNA electrochemical interface biosensor based on screen-printed carbon electrodes (SPCEs) holds promise for point-of-care testing (POCT) detection of pathogens in food safety. Nevertheless, SPCE commonly has a rough surface and suffers from relatively low electron transfer rate, disorder capture probes (CPs), the steric hindrance effect target nucleic acid binding. These issues lead to sensitivity. Herein, simple rapid N-doped porous (NPC)-modified freezing-directed combined with catalyzed hairpin assembly (CHA) was constructed one-pot samples without time-consuming growth cultures. The by modified NPC enhanced properties, CP designed CHA stably fixed electrode high hybridization efficiency. Moreover, signals amplified enable selective sensitive washing steps. This method is wide linear range 101 107 CFU/mL limit 5 Escherichia coli shows specificity against other coexisting pathogens. whole complex performed only within 60 min sample-to-answer, which great potential POCT
Language: Английский
Citations
0International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140928 - 140928
Published: Feb. 1, 2025
Language: Английский
Citations
0Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162049 - 162049
Published: April 1, 2025
Language: Английский
Citations
0Bioelectrochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108984 - 108984
Published: April 1, 2025
Language: Английский
Citations
0Small Methods, Journal Year: 2025, Volume and Issue: unknown
Published: April 10, 2025
Abstract Here a novel digital bioassay readout concept is reported that does not rely on enzymatic amplification nor compartmenting of an analyzed liquid sample. Rather, it based counting individual affinity‐captured target biomolecules via the use tethered catalytic hairpin assembly (tCHA) deployed solid sensor surface with spatial confinement utilized by flexible polymer linker (FPL). Wide‐field plasmon‐enhanced fluorescence (PEF) imaging employed for optical real‐time probing reaction kinetics, where molecules are manifested as spatially distinct bright fluorescent spots. The effect length FPLs investigated, and analytical performance dual tCHA‐PEF tested using model short single‐stranded DNA analyte. When applied in sandwich immunoassay, detection proteins at sub‐femtomolar concentrations demonstrated. experiments supported diffusion‐limited mass transfer models document potential new class generic enzyme‐free bioanalytical tools enabling ultrasensitive analysis trace amounts protein nucleic acid analytes, making attractive future molecular diagnostics research applications.
Language: Английский
Citations
0Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 282, P. 117497 - 117497
Published: April 18, 2025
Language: Английский
Citations
0Microchemical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 111948 - 111948
Published: Oct. 1, 2024
Language: Английский
Citations
3Analytical Methods, Journal Year: 2024, Volume and Issue: 16(43), P. 7319 - 7324
Published: Jan. 1, 2024
A multi-detection strategy based on branched DNA nanostructures was developed to achieve the simultaneous high specificity identification and highly sensitive fluorescence responses HBV antigen DNA.
Language: Английский
Citations
1