Micromachines,
Год журнала:
2024,
Номер
15(12), С. 1509 - 1509
Опубликована: Дек. 18, 2024
The
increased
demand
for
consuming
seafood
has
made
production
undergo
a
rapid
period
of
growth.
However,
high
risk
contamination
from
harmful
microorganisms
and
marine
toxins
which
can
cause
health
problems
humans
it.
Concerning
this
issue,
monitoring
safety
become
center
attention
researchers,
developing
effective
methods
detecting
in
critical
research
field.
In
context,
biosensors
have
served
as
promising
approach
to
monitor
contamination.
Compared
conventional
methods,
some
key
benefits
such
sensitivity,
selectivity,
portability,
user-friendly
operation.
Along
with
significant
advances
biosensors,
processes
be
simplified
performed
outside
the
laboratory.
review
article,
we
describe
mechanisms
two
main
types
regarding
electrochemical
optical
biosensors.
current
reports
within
last
five
years
on
application
these
are
also
summarized.
Talanta Open,
Год журнала:
2024,
Номер
9, С. 100327 - 100327
Опубликована: Май 16, 2024
Gold
nanoparticles
(AuNPs)
have
become
the
focus
of
rapid
research
due
to
their
unique
optical
and
electronic
properties.
There
has
been
a
noticeable
increase
in
papers
relating
AuNPs,
with
over
71,000
publications
between
2019
2024.
AuNPs
possess
exceptional
stability,
low
resistance,
high
conductivity,
extensive
light
interaction,
making
them
well-suited
for
biological
sensing
applications.
This
literature
study
begins
by
examining
different
approaches
synthesizing
including
chemical,
physical,
methods,
before
exploring
use
as
biosensors.
A
comprehensive
examination
various
detection
localized
surface
plasmon
resonance
(LSPR),
luminescence,
surface-enhanced
Raman
scattering
(SERS),
(SPR),
electrochemical
properties,
is
facilitated
through
an
exploration
fundamental
principles
classification
We
primarily
on
using
medical
diagnostics,
highlighting
current
advancements
disease
AuNPs-based
biosensors
conditions
like
COVID-19,
dengue
fever,
diabetes.
The
review
emphasizes
achievements
synthesis
bright
prospects
biocompatible
efficient
biosensor
Sensors,
Год журнала:
2024,
Номер
24(20), С. 6701 - 6701
Опубликована: Окт. 18, 2024
This
work
reported
gold
nanoparticles
(AuNPs)-based
colorimetric
immunoassay
with
the
Cu-based
metal–organic
framework
(MOF)
to
load
pyrroloquinoline
quinone
(PQQ)
for
catalytic
oxidation
of
cysteine.
In
this
method,
both
Cu2+
and
PQQ
in
MOF
could
promote
inducer
cysteine
by
redox
cycling,
thus
limiting
cysteine-induced
aggregation
AuNPs
achieving
dual
signal
amplification.
Specifically,
recombinant
carcinoembryonic
antigen
(CEA)
targets
were
anchored
on
through
metal
coordination
interactions
between
hexahistidine
(His6)
tag
CEA
unsaturated
sites
MOF.
The
CEA/PQQ-loaded
be
captured
antibody-coated
ELISA
plate
catalyze
However,
once
target
samples
bound
antibody
immobilized
surface,
attachment
would
limited.
Cysteine
remaining
solution
trigger
cause
a
color
change
from
red
blue.
concentration
was
positively
related
AuNPs.
signal-on
competitive
plasmonic
exhibited
low
detection
limit
linear
range
0.01–1
ng/mL.
Note
that
most
proteins
commercial
kits
are
His6
N-
or
C-terminal,
so
provide
sensitive
platform
biomarkers.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 20, 2025
In
recent
years,
entropy-driven
circuit
(EDC)
dynamic
DNA
networks
have
garnered
significant
attention
in
nucleic
acid
detection
owing
to
their
simplicity,
efficiency,
and
flexible
design.
Nevertheless,
conventional
EDC
reactions
face
a
constraint
achieving
optimal
signal
amplification
due
solitary
feeble
driving
force.
To
overcome
this
limitation,
we
innovatively
devised
gold
nanoparticle
(AuNP)
dispersion-enhanced
(Au-EDC)
approach,
pioneering
novel
colorimetric
output
system.
The
system
was
harmoniously
integrated
with
the
ligase
chain
reaction
(LCR)
for
precise
single
nucleotide
polymorphism
(SNP)
genotyping.
Specifically,
LCR
selectively
executed
solely
on
positive
strand
of
mutant
target
(MT),
facilitating
point-to-strand
information
transduction.
Subsequently,
product
triggered
Au-EDC
cycling
reaction,
causing
DNA-AuNPs
network
progressively
disintegrate
release
pronounced
signal.
This
strategic
design
ingeniously
harnessed
entropy
increase
that
occurs
as
AuNPs
undergo
transition
from
aggregated
dispersed
states,
offering
supplemental
impetus
cycle.
LCR-Au-EDC
excelled
detecting
MT
at
concentrations
low
320
fM
differentiating
pooled
samples
mutation
frequencies
0.1%.
Moreover,
accurately
performed
SNP
genotyping
real
genomes
derived
soybean
leaves.
Consequently,
study
not
only
develops
sensing
based
but
also
provides
cost-effective
efficient
tool.
Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Апрель 23, 2025
Integrating
nucleic
acids
(NAs)
with
nanomaterials
has
substantially
advanced
biomedical
research,
enabling
critical
applications
in
biosensing,
drug
delivery,
therapeutics,
and
the
synthesis
of
nanomaterials.
At
core
these
advances
are
reactions
NAs
on
nanomaterial
surfaces,
encompassing
conjugation
(covalent
non-covalent),
detachment
(physical
chemical),
signal
amplification
(enzyme-mediated
amplification,
enzyme-free
DNA
Walker).
Here,
we
review
fundamental
mechanisms
recent
progress
acid
discuss
emerging
for
diagnostics,
nanomedicine,
gene
therapy,
explore
persistent
challenges
field.
We
offer
a
forward-looking
perspective
how
future
developments
could
better
control,
optimize,
harness
transformative
nanomedicine
engineering.
