Tailored Fabrication of 3D Nanopores Made of Dielectric Oxides for Multiple Nanoscale Applications DOI

Germàn Lanzavecchia,

Аnastasiia S. Sapunova, Ali Douaki

и другие.

Nano Letters, Год журнала: 2024, Номер 24(33), С. 10098 - 10105

Опубликована: Авг. 9, 2024

Solid-state nanopores are a key platform for single-molecule detection and analysis that allow engineering of their properties by controlling size, shape, chemical functionalization. However, approaches relying on polymers have limits what concerns hardness, robustness, durability, refractive index. Nanopores made oxides with high dielectric constant would overcome such the potential to extend suitability solid-state toward optoelectronic technologies. Here, we present versatile method fabricate three-dimensional different convex, straight, concave shapes demonstrate functionality in series technologies applications as ionic nanochannels, current rectification, memristors, DNA sensing. Our experimental data supported numerical simulations showcase effect oxide materials. This approach robust tunable can be extended other 3D variety dielectrics.

Язык: Английский

Precise Structural Analysis of Neutral Glycans Using Aerolysin Mutant T240R Nanopore DOI
Wenqi Lu, Xinjia Zhao, Minmin Li

и другие.

ACS Nano, Год журнала: 2024, Номер 18(19), С. 12412 - 12426

Опубликована: Май 2, 2024

Glycans play vital roles in nearly all life processes of multicellular organisms, and understanding these activities is inseparable from elucidating the biological significance glycans. However, glycan research has lagged behind that DNA protein due to challenges posed by structural heterogeneity isomerism (i.e., structures with equal molecular weights) lack high-efficiency analysis techniques. Nanopore technology emerged as a sensitive single-molecule biosensor, shining light on analysis. significant number glycans are small uncharged, making it challenging elicit identifiable nanopore signals. Here we introduce

Язык: Английский

Процитировано

11

Resolving Sulfation Posttranslational Modifications on a Peptide Hormone using Nanopores DOI Creative Commons
Xiuqi Chen, Jasper W. van de Sande, Justas Ritmejeris

и другие.

ACS Nano, Год журнала: 2024, Номер 18(42), С. 28999 - 29007

Опубликована: Окт. 10, 2024

Peptide hormones are decorated with post-translational modifications (PTMs) that crucial for receptor recognition. Tyrosine sulfation on plant peptide is, example, essential growth and development. Measuring the occurrence position of sulfotyrosine however, compromised by major technical challenges during isolation detection. Nanopores can sensitively detect protein PTMs at single-molecule level. By translocating PTM variants pentapeptide hormone phytosulfokine (PSK) through a nanopore, we here demonstrate accurate identification phosphorylation two tyrosine residues PSK. Sulfation be clearly detected distinguished (>90%) from same residue. Moreover, presence or absence close-by accurately determined (>96% accuracy). Our findings extraordinary sensitivity nanopore measurements, providing powerful tool identifying position-specific promising wider applications to identify PTMs.

Язык: Английский

Процитировано

6

Smart polymers advance analytical chemistry DOI
Yahui Zhang, Xinjia Zhao, Guangyan Qing

и другие.

TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118198 - 118198

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0

Nanopore toward Genuine Single-Molecule Sensing: Molecular Ping-Pong Technology DOI
Xinjia Zhao, Yahui Zhang, Guangyan Qing

и другие.

Nano Letters, Год журнала: 2025, Номер unknown

Опубликована: Фев. 26, 2025

Nanopore sensing is a so-called label-free, single-molecule technology; however, multiple events of different molecules are recorded to obtain statistically robust data, which can limit both efficiency and sample use. To overcome these challenges, nanopore molecular ping-pong technology enables precise manipulation, reducing systematic stochastic errors by repeatedly measuring the same molecule. This review introduces fundamentals advancements technology, highlighting recent breakthrough achieving over 10,000 recaptures single dsDNA molecule within minutes. innovation not only minimizes requirements, critical for nonamplifiable samples, but also significantly enhances experimental precision. While current applications focus on dsDNA, extending this protein glycan analysis could transform research. Just as revolutionized DNA sequencing, it holds potential drive development nanopore-based sequencers, paving way groundbreaking in biology biomedicine.

Язык: Английский

Процитировано

0

In‐Tip Nanoreactors for Simultaneous Proteolysis and Enrichment of Phosphorylated Peptides DOI Open Access

Ling Yan

Analytical Science Advances, Год журнала: 2025, Номер 6(1)

Опубликована: Март 12, 2025

ABSTRACT Protein phosphorylation introduces negative charges on the hydroxyl groups of serine, threonine, and tyrosine residues, reducing ionization efficiency phosphorylated peptides. The low abundance peptides often diminishes their detection using mass spectrometry. To enhance identification low‐abundance peptides, an enrichment step was used, which complicated high‐throughput analysis proteomes. In this study, we developed a titanium dioxide surface‐modified macroporous silicon encapsulated micropipette tips, loaded with trypsin, to integrate rapid enzymatic protein hydrolysis selective extraction within microfluidic enzyme reactor. This streamlined approach simplified sample preparation process, combining hydrolysis, separation while maintaining high efficiency. method enabled comprehensive complex cancer cell line samples in 1–2 h. Successful from mixtures achieved matrix‐assisted laser desorption/ionization‐time‐of‐flight‐mass application may provide potential for phosphoproteomics advance study modifications.

Язык: Английский

Процитировано

0

Single molecular profile of proteins sensing by nanopore technology DOI
Lingzhi Wu, Xingyue Luo, Ke Qi

и другие.

Talanta, Год журнала: 2025, Номер unknown, С. 128040 - 128040

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Protein Profiling by Nanopore-Based Technology DOI

Fu-Na Meng,

Xin Li,

Na Zou

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 6, 2025

Proteins are the molecular foundations of life and disease responsible for understanding most biological processes. Nanopore technology devoted to revealing single-molecule behavior has made great breakthroughs protein identification, detection analysis, including sequencing. Here, we present an overview latest advances in profiling by nanopores from identification quantification biomarkers enzymes delineation conformations interactions at level, focused on diverse exciting approaches Furthermore, discuss primary challenges associated with nanopore-based sensing recommend potential strategies respond these perspective nanopore engineering data processing.

Язык: Английский

Процитировано

0

Trends in nucleic acids and non-nucleic acids nanostructures-enhanced site-specific DNA methylation biosensing DOI
Yongchao Yao, Limei Zhang, Xun He

и другие.

TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118315 - 118315

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0

Characterization and modulation of the unimolecular conformation of integrins with nanopore sensors DOI
Rongjie Li, Daixin Liu,

Meili Ren

и другие.

Chemical Engineering Journal, Год журнала: 2024, Номер 492, С. 152374 - 152374

Опубликована: Май 19, 2024

Язык: Английский

Процитировано

1

MNPs-aptazymes-PtNPs molecular motor biosensor mediated by circular cleavage reactions for the ultrasensitive detection of aflatoxin B1 in real samples DOI
Wanchong He, Ce Liu, Jing Han

и другие.

Microchemical Journal, Год журнала: 2024, Номер unknown, С. 112007 - 112007

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

1