Nano Letters,
Год журнала:
2024,
Номер
24(33), С. 10098 - 10105
Опубликована: Авг. 9, 2024
Solid-state
nanopores
are
a
key
platform
for
single-molecule
detection
and
analysis
that
allow
engineering
of
their
properties
by
controlling
size,
shape,
chemical
functionalization.
However,
approaches
relying
on
polymers
have
limits
what
concerns
hardness,
robustness,
durability,
refractive
index.
Nanopores
made
oxides
with
high
dielectric
constant
would
overcome
such
the
potential
to
extend
suitability
solid-state
toward
optoelectronic
technologies.
Here,
we
present
versatile
method
fabricate
three-dimensional
different
convex,
straight,
concave
shapes
demonstrate
functionality
in
series
technologies
applications
as
ionic
nanochannels,
current
rectification,
memristors,
DNA
sensing.
Our
experimental
data
supported
numerical
simulations
showcase
effect
oxide
materials.
This
approach
robust
tunable
can
be
extended
other
3D
variety
dielectrics.
ACS Nano,
Год журнала:
2024,
Номер
18(19), С. 12412 - 12426
Опубликована: Май 2, 2024
Glycans
play
vital
roles
in
nearly
all
life
processes
of
multicellular
organisms,
and
understanding
these
activities
is
inseparable
from
elucidating
the
biological
significance
glycans.
However,
glycan
research
has
lagged
behind
that
DNA
protein
due
to
challenges
posed
by
structural
heterogeneity
isomerism
(i.e.,
structures
with
equal
molecular
weights)
lack
high-efficiency
analysis
techniques.
Nanopore
technology
emerged
as
a
sensitive
single-molecule
biosensor,
shining
light
on
analysis.
significant
number
glycans
are
small
uncharged,
making
it
challenging
elicit
identifiable
nanopore
signals.
Here
we
introduce
ACS Nano,
Год журнала:
2024,
Номер
18(42), С. 28999 - 29007
Опубликована: Окт. 10, 2024
Peptide
hormones
are
decorated
with
post-translational
modifications
(PTMs)
that
crucial
for
receptor
recognition.
Tyrosine
sulfation
on
plant
peptide
is,
example,
essential
growth
and
development.
Measuring
the
occurrence
position
of
sulfotyrosine
however,
compromised
by
major
technical
challenges
during
isolation
detection.
Nanopores
can
sensitively
detect
protein
PTMs
at
single-molecule
level.
By
translocating
PTM
variants
pentapeptide
hormone
phytosulfokine
(PSK)
through
a
nanopore,
we
here
demonstrate
accurate
identification
phosphorylation
two
tyrosine
residues
PSK.
Sulfation
be
clearly
detected
distinguished
(>90%)
from
same
residue.
Moreover,
presence
or
absence
close-by
accurately
determined
(>96%
accuracy).
Our
findings
extraordinary
sensitivity
nanopore
measurements,
providing
powerful
tool
identifying
position-specific
promising
wider
applications
to
identify
PTMs.
Nanopore
sensing
is
a
so-called
label-free,
single-molecule
technology;
however,
multiple
events
of
different
molecules
are
recorded
to
obtain
statistically
robust
data,
which
can
limit
both
efficiency
and
sample
use.
To
overcome
these
challenges,
nanopore
molecular
ping-pong
technology
enables
precise
manipulation,
reducing
systematic
stochastic
errors
by
repeatedly
measuring
the
same
molecule.
This
review
introduces
fundamentals
advancements
technology,
highlighting
recent
breakthrough
achieving
over
10,000
recaptures
single
dsDNA
molecule
within
minutes.
innovation
not
only
minimizes
requirements,
critical
for
nonamplifiable
samples,
but
also
significantly
enhances
experimental
precision.
While
current
applications
focus
on
dsDNA,
extending
this
protein
glycan
analysis
could
transform
research.
Just
as
revolutionized
DNA
sequencing,
it
holds
potential
drive
development
nanopore-based
sequencers,
paving
way
groundbreaking
in
biology
biomedicine.
Analytical Science Advances,
Год журнала:
2025,
Номер
6(1)
Опубликована: Март 12, 2025
ABSTRACT
Protein
phosphorylation
introduces
negative
charges
on
the
hydroxyl
groups
of
serine,
threonine,
and
tyrosine
residues,
reducing
ionization
efficiency
phosphorylated
peptides.
The
low
abundance
peptides
often
diminishes
their
detection
using
mass
spectrometry.
To
enhance
identification
low‐abundance
peptides,
an
enrichment
step
was
used,
which
complicated
high‐throughput
analysis
proteomes.
In
this
study,
we
developed
a
titanium
dioxide
surface‐modified
macroporous
silicon
encapsulated
micropipette
tips,
loaded
with
trypsin,
to
integrate
rapid
enzymatic
protein
hydrolysis
selective
extraction
within
microfluidic
enzyme
reactor.
This
streamlined
approach
simplified
sample
preparation
process,
combining
hydrolysis,
separation
while
maintaining
high
efficiency.
method
enabled
comprehensive
complex
cancer
cell
line
samples
in
1–2
h.
Successful
from
mixtures
achieved
matrix‐assisted
laser
desorption/ionization‐time‐of‐flight‐mass
application
may
provide
potential
for
phosphoproteomics
advance
study
modifications.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 6, 2025
Proteins
are
the
molecular
foundations
of
life
and
disease
responsible
for
understanding
most
biological
processes.
Nanopore
technology
devoted
to
revealing
single-molecule
behavior
has
made
great
breakthroughs
protein
identification,
detection
analysis,
including
sequencing.
Here,
we
present
an
overview
latest
advances
in
profiling
by
nanopores
from
identification
quantification
biomarkers
enzymes
delineation
conformations
interactions
at
level,
focused
on
diverse
exciting
approaches
Furthermore,
discuss
primary
challenges
associated
with
nanopore-based
sensing
recommend
potential
strategies
respond
these
perspective
nanopore
engineering
data
processing.
