Bioprinting of Cells, Organoids and Organs-on-a-Chip Together with Hydrogels Improves Structural and Mechanical Cues
Cells,
Год журнала:
2024,
Номер
13(19), С. 1638 - 1638
Опубликована: Окт. 1, 2024
The
3D
bioprinting
technique
has
made
enormous
progress
in
tissue
engineering,
regenerative
medicine
and
research
into
diseases
such
as
cancer.
Apart
from
individual
cells,
a
collection
of
organoids,
can
be
printed
combination
with
various
hydrogels.
It
hypothesized
that
will
even
become
promising
tool
for
mechanobiological
analyses
organoids
their
matrix
environments
highly
defined
precisely
structured
environments,
which
the
mechanical
properties
cell
environment
individually
adjusted.
Mechanical
obstacles
or
bead
markers
integrated
bioprinted
samples
to
analyze
deformations
forces
within
these
constructs,
perform
biophysical
analysis
complex
systems,
are
still
not
standard
techniques.
review
highlights
advances
4D
printing
technologies
integrating
cues
so
next
step
detailed
key
future
directions
organoid
generation
development
disease
model
regeneration
drug
testing
perspective.
Finally,
hydrogels,
pure
natural
synthetic
hydrogels
mixtures,
organoid–cell
co-cultures,
organ-on-a-chip
systems
organoid-organ-on-a
chip
combinations
introduces
use
assembloids
determine
mutual
interactions
different
types
cell–matrix
interferences
specific
biological
environments.
Язык: Английский
Prospects of emerging 3D bioprinting technologies: major technology components, technology developers, and end users—Part I
MGM Journal of Medical Sciences,
Год журнала:
2024,
Номер
11(2), С. 331 - 339
Опубликована: Апрель 1, 2024
Abstract
Bioprinting
technology
aims
to
create
3D
structures
with
living
cells
mimic
real
tissue
and
organ
functions.
The
process
involves
various
additive
technologies,
including
bioprinters
bioinks.
Bioinks
comprise
hydrogels,
scaffolds,
additives,
growth
factors,
cells.
While
much
of
the
is
still
in
exploratory
stages,
it
has
successfully
produced
tissue,
blood
vessels,
bones.
Research
suggests
potential
for
bioprinting
whole
organs
revolutionize
medical
procedures.
Current
major
business
components
include
types
advanced
Advancements
technologies
such
as
cellular
reprogramming
hold
enhance
development
superior
bioinks,
thereby
enabling
fabrication
bioprinted
tissues.
offers
significant
benefits
across
research,
personalized
medicine,
other
applications.
This
review
provides
a
flavor
using
areas
usage,
Development
(R&D),
its
applications
on
wide
spectrum,
medicine.
Язык: Английский
Modelling Cancer Pathophysiology: Mechanisms and Changes in the Extracellular Matrix During Cancer Initiation and Early Tumour Growth
Cancers,
Год журнала:
2025,
Номер
17(10), С. 1675 - 1675
Опубликована: Май 15, 2025
Cancer
initiation
and
early
tumour
growth
are
complex
processes
influenced
by
multiple
cellular
microenvironmental
factors.
A
critical
aspect
of
progression
is
the
dynamic
interplay
between
cancer
cells
extracellular
matrix
(ECM),
which
undergoes
significant
alterations
to
support
malignancy.
The
loss
cell
polarity
an
hallmark
progression,
disrupting
normal
tissue
architecture
fostering
cancerous
transformation.
Circumstantially,
cancer-associated
microRNAs
(miRNAs)
regulate
key
oncogenic
processes,
including
ECM
remodelling,
epithelial-to-mesenchymal
transition
(EMT),
tumorigenic
vascular
development,
further
driving
growth.
alterations,
particularly
changes
in
stiffness
mechanotransduction
signals,
create
a
supportive
niche
for
cells,
enhancing
their
survival,
proliferation,
invasion.
EMT
its
subtype,
epithelial-to-endothelial
(EET),
contribute
plasticity,
promote
generation
stem
(CSCs),
vascularisation.
Furthermore,
development
like
vasculogenesis
angiogenesis
sustaining
growth,
supplying
oxygen
nutrients
hypoxic
malignant
within
evolving
microenvironments.
This
review
explores
mechanisms
underlying
these
microenvironments,
with
emphasis
on
collective
role
It
will
delve
into
present
vitro
modelling
strategies
developed
closely
mimic
pathophysiology.
Understanding
crucial
developing
targeted
therapies
aimed
at
cancer-promoting
pathways
improving
clinical
outcomes.
Язык: Английский
Recent advancements and challenges in 3D bioprinting for cancer applications
Bioprinting,
Год журнала:
2024,
Номер
43, С. e00357 - e00357
Опубликована: Сен. 13, 2024
Язык: Английский
3D PRINTING OF CANCER MODELS FOR DRUG DISCOVERY: ADVANCEMENTS, CHALLENGES, AND FUTURE PERSPECTIVES
Journal of Medicine Surgery and Public Health,
Год журнала:
2024,
Номер
unknown, С. 100165 - 100165
Опубликована: Дек. 1, 2024
Язык: Английский
Exploring Current In Vitro Models for Cancer Research
Interdisciplinary cancer research,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
This
chapter
presents
an
extensive
investigation
into
the
diverse
landscape
of
in
vitro
models
tailored
for
unraveling
complexities
tumors.
The
exploration
unfolds
with
a
meticulous
examination
two-dimensional
(2D)
tumor
Sect.
2,
providing
comprehensive
understanding
their
distinct
characteristics
and
inherent
limitations.
narrative
then
seamlessly
transitions
to
3,
where
focus
broadens
encompass
three-dimensional
(3D)
models,
exploring
realms
spheroids,
organoids,
ex
vivo
explant
(Sects.
3.1,
3.2,
3.3).
delves
frontier
bioprinting
3.4,
elucidating
its
applications
addressing
challenges
associated
this
cutting-edge
technology.
Section
3.5
investigates
pivotal
role
microfluidics
cancer
research,
underscoring
contributions
mimicking
microenvironment.
culminates
3.6,
dedicated
involving
chorioallantoic
membrane,
shedding
light
on
unique
attributes
significant
studies.
overview
aims
elevate
our
comprehension
current
spectrum
models.
By
delineating
nuanced
each
model
type,
provides
valuable
insights
that
are
poised
influence
methodologies
future
research.
Emphasizing
collective
impact
these
advancing
preclinical
studies,
serves
as
foundational
resource,
fostering
innovation
informed
decision-making
development
more
effective
therapeutic
strategies
treatment.
Язык: Английский
Editorial Note: Biomaterials in Cancer - From Research Breakthroughs to Clinical Implementation
Translational Oncology,
Год журнала:
2024,
Номер
48, С. 102061 - 102061
Опубликована: Июль 27, 2024
Язык: Английский
Revolutionizing oral cancer research with 3D bioprinting technology
Oral Oncology Reports,
Год журнала:
2024,
Номер
10, С. 100463 - 100463
Опубликована: Апрель 26, 2024
Язык: Английский
4D EPR Oximetry Imaging for 4D Bioprinting and Tissue Engineering
Опубликована: Янв. 1, 2024
Bioprinting,
akin
to
3D
printing,
employs
cell-laden
hydrogels
such
as
GelMA
(Gelatine
Methacrylate)
and
Alginate
instead
of
plastic
or
resin
create
biological
structures.
The
printing
process
followed
by
subsequent
tissue
maturation
is
known
4D
bioprinting,
where
the
fourth
dimension
time.
Ensuring
adequate
consistent
oxygen,
O2,
supply
within
bioprint
volume
over
entire
period
crucial,
even
transient
hypoxia
can
impact
cellular
behavior
phenotypes.
However,
fully
developed
vasculature
remains
an
unresolved
technical
challenge.
Several
approaches
chemically
(peroxides)
biologically
(photosynthesis)
generate
oxygen
during
have
been
proposed.
a
comprehensive
evaluation
optimization
these
methods
not
currently
possible
due
lack
quantitative
longitudinal
O2
imaging
modality.
electron
paramagnetic
resonance
(EPRI)
demonstrated
be
method
choice
solve
problem
oxygenation
in
prolonged
EPRI
permits
noninvasive
mapping
using
incorporate
bioinks
probes.
Two
types
probes
utilized:
water
soluble
triarylmehyl
(trityls)
stable
radicals
solid
lithium
octa-nbutoxy-phthalocyanine
(LiNc-BuO)
particles.
Incorporation
materials
modifies
its
chemical
optical
properties.
For
example,
LiNc-BuO
absorbing
light
affects
photopolymerization.
Also,
bioprinting
Trityls
react
with
formed
intermediate
radicals.
Part
presented
work
was
dedicated
understanding
that
includes
addition
into
commonly
used
GelMa
alginate
bioinks.
Bioprinting
done
locally
digital
processing
(DLP)
extrusion
bioprinters.
oximetry
performed
built
instrument.
Both
acellular
constructs
were
successfully
printed
imaged.
Experiments
revealed
previously
unreported
phenomenon
depletion
presence
residual
photoinitiator.
Oxygen
consumption
rates
HEK293T
cells
structures
quantified.
changing
levels
murine
macrophage-laden
freeform
reversible
embedding
suspended
(FRESH)
methodology.
computed
analyzing
consecutive
EPR
images
time
dimension.
Given
incorporation
bioink,
proper
control
experiments
evaluating
temporal
probe
stability
signal
intensity
bioink
conducted.
In
conclusion,
we've
shown
feasibility
integrating
paving
way
for
diverse
applications
various
molecular
spin
These
findings
are
foundational
advancing
technologies
dynamics
engineering.
Язык: Английский
Modeling intratumor heterogeneity in breast cancer
Biofabrication,
Год журнала:
2024,
Номер
17(1), С. 012009 - 012009
Опубликована: Дек. 6, 2024
Reduced
therapy
response
in
breast
cancer
has
been
correlated
with
heterogeneity
biomarker
composition,
expression
level,
and
spatial
distribution
of
cells
within
a
patient
tumor.
Thus,
there
is
need
for
models
to
replicate
cell-cell,
cell-stromal,
cell-microenvironment
interactions
during
progression.
Traditional
two-dimensional
(2D)
cell
culture
are
convenient
but
cannot
adequately
represent
tumor
microenvironment
histological
organization,in
vivo3D
spatial/cellular
context,
physiological
relevance.
Recently,
three-dimensional
(3D)in
vitrotumor
have
shown
provide
an
improved
platform
incorporating
compositional
better
mimic
the
biological
characteristics
tumors
assess
drug
response.
Advances
3D
bioprinting
allowed
creation
more
complex
physiologic
representation
while
controlling
reproducibility
accuracy.
This
review
aims
summarize
advantages
challenges
current
3Din
vitromodels
evaluating
cancer,
particular
emphasis
on
bioprinting,
addresses
several
key
issues
future
model
development
as
well
their
application
other
cancers.
Язык: Английский