Oral Oncology Reports, Journal Year: 2024, Volume and Issue: 10, P. 100463 - 100463
Published: April 26, 2024
Language: Английский
Cells, Journal Year: 2024, Volume and Issue: 13(19), P. 1638 - 1638
Published: Oct. 1, 2024
The 3D bioprinting technique has made enormous progress in tissue engineering, regenerative medicine and research into diseases such as cancer. Apart from individual cells, a collection of organoids, can be printed combination with various hydrogels. It hypothesized that will even become promising tool for mechanobiological analyses organoids their matrix environments highly defined precisely structured environments, which the mechanical properties cell environment individually adjusted. Mechanical obstacles or bead markers integrated bioprinted samples to analyze deformations forces within these constructs, perform biophysical analysis complex systems, are still not standard techniques. review highlights advances 4D printing technologies integrating cues so next step detailed key future directions organoid generation development disease model regeneration drug testing perspective. Finally, hydrogels, pure natural synthetic hydrogels mixtures, organoid–cell co-cultures, organ-on-a-chip systems organoid-organ-on-a chip combinations introduces use assembloids determine mutual interactions different types cell–matrix interferences specific biological environments.
Language: Английский
Citations
6
MGM Journal of Medical Sciences, Journal Year: 2024, Volume and Issue: 11(2), P. 331 - 339
Published: April 1, 2024
Abstract Bioprinting technology aims to create 3D structures with living cells mimic real tissue and organ functions. The process involves various additive technologies, including bioprinters bioinks. Bioinks comprise hydrogels, scaffolds, additives, growth factors, cells. While much of the is still in exploratory stages, it has successfully produced tissue, blood vessels, bones. Research suggests potential for bioprinting whole organs revolutionize medical procedures. Current major business components include types advanced Advancements technologies such as cellular reprogramming hold enhance development superior bioinks, thereby enabling fabrication bioprinted tissues. offers significant benefits across research, personalized medicine, other applications. This review provides a flavor using areas usage, Development (R&D), its applications on wide spectrum, medicine.
Language: Английский
Citations
4
Bioprinting, Journal Year: 2024, Volume and Issue: 43, P. e00357 - e00357
Published: Sept. 13, 2024
Language: Английский
Citations
1
Journal of Medicine Surgery and Public Health, Journal Year: 2024, Volume and Issue: unknown, P. 100165 - 100165
Published: Dec. 1, 2024
Language: Английский
Citations
1
Interdisciplinary cancer research, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
This chapter presents an extensive investigation into the diverse landscape of in vitro models tailored for unraveling complexities tumors. The exploration unfolds with a meticulous examination two-dimensional (2D) tumor Sect. 2, providing comprehensive understanding their distinct characteristics and inherent limitations. narrative then seamlessly transitions to 3, where focus broadens encompass three-dimensional (3D) models, exploring realms spheroids, organoids, ex vivo explant (Sects. 3.1, 3.2, 3.3). delves frontier bioprinting 3.4, elucidating its applications addressing challenges associated this cutting-edge technology. Section 3.5 investigates pivotal role microfluidics cancer research, underscoring contributions mimicking microenvironment. culminates 3.6, dedicated involving chorioallantoic membrane, shedding light on unique attributes significant studies. overview aims elevate our comprehension current spectrum models. By delineating nuanced each model type, provides valuable insights that are poised influence methodologies future research. Emphasizing collective impact these advancing preclinical studies, serves as foundational resource, fostering innovation informed decision-making development more effective therapeutic strategies treatment.
Language: Английский
Citations
0
Translational Oncology, Journal Year: 2024, Volume and Issue: 48, P. 102061 - 102061
Published: July 27, 2024
Language: Английский
Citations
0
Published: Jan. 1, 2024
Bioprinting, akin to 3D printing, employs cell-laden hydrogels such as GelMA (Gelatine Methacrylate) and Alginate instead of plastic or resin create biological structures. The printing process followed by subsequent tissue maturation is known 4D bioprinting, where the fourth dimension time. Ensuring adequate consistent oxygen, O2, supply within bioprint volume over entire period crucial, even transient hypoxia can impact cellular behavior phenotypes. However, fully developed vasculature remains an unresolved technical challenge. Several approaches chemically (peroxides) biologically (photosynthesis) generate oxygen during have been proposed. a comprehensive evaluation optimization these methods not currently possible due lack quantitative longitudinal O2 imaging modality. electron paramagnetic resonance (EPRI) demonstrated be method choice solve problem oxygenation in prolonged EPRI permits noninvasive mapping using incorporate bioinks probes. Two types probes utilized: water soluble triarylmehyl (trityls) stable radicals solid lithium octa-nbutoxy-phthalocyanine (LiNc-BuO) particles. Incorporation materials modifies its chemical optical properties. For example, LiNc-BuO absorbing light affects photopolymerization. Also, bioprinting Trityls react with formed intermediate radicals. Part presented work was dedicated understanding that includes addition into commonly used GelMa alginate bioinks. Bioprinting done locally digital processing (DLP) extrusion bioprinters. oximetry performed built instrument. Both acellular constructs were successfully printed imaged. Experiments revealed previously unreported phenomenon depletion presence residual photoinitiator. Oxygen consumption rates HEK293T cells structures quantified. changing levels murine macrophage-laden freeform reversible embedding suspended (FRESH) methodology. computed analyzing consecutive EPR images time dimension. Given incorporation bioink, proper control experiments evaluating temporal probe stability signal intensity bioink conducted. In conclusion, we've shown feasibility integrating paving way for diverse applications various molecular spin These findings are foundational advancing technologies dynamics engineering.
Language: Английский
Citations
0
Biofabrication, Journal Year: 2024, Volume and Issue: 17(1), P. 012009 - 012009
Published: Dec. 6, 2024
Reduced therapy response in breast cancer has been correlated with heterogeneity biomarker composition, expression level, and spatial distribution of cells within a patient tumor. Thus, there is need for models to replicate cell-cell, cell-stromal, cell-microenvironment interactions during progression. Traditional two-dimensional (2D) cell culture are convenient but cannot adequately represent tumor microenvironment histological organization,in vivo3D spatial/cellular context, physiological relevance. Recently, three-dimensional (3D)in vitrotumor have shown provide an improved platform incorporating compositional better mimic the biological characteristics tumors assess drug response. Advances 3D bioprinting allowed creation more complex physiologic representation while controlling reproducibility accuracy. This review aims summarize advantages challenges current 3Din vitromodels evaluating cancer, particular emphasis on bioprinting, addresses several key issues future model development as well their application other cancers.
Language: Английский
Citations
0
Oral Oncology Reports, Journal Year: 2024, Volume and Issue: 10, P. 100463 - 100463
Published: April 26, 2024
Language: Английский
Citations
0