International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142654 - 142654
Опубликована: Март 1, 2025
Язык: Английский
International Journal of Biological Macromolecules, Год журнала: 2025, Номер 297, С. 139930 - 139930
Опубликована: Янв. 15, 2025
Язык: Английский
Процитировано
1
Journal of Food Science, Год журнала: 2025, Номер 90(2)
Опубликована: Фев. 1, 2025
Gualou (Trichosanthes kirilowii Maxim) and Xiebai (Allium macrostemon Bunge) have been extensively studied for their diverse active ingredients, which include flavonoids, polysaccharides, triterpenoids. This study employed a single-factor experiment alongside the Plackett-Burman design utilized response surface methodology to optimize ultrasonic-enzyme-assisted extraction (U-EAT) method functional components in (GLXB). The optimal conditions were identified as solid-to-liquid ratio of 1:11 g/mL, an alcohol concentration 55%, enzyme 1.2%, temperature 40°C. yields triterpenoids obtained 0.94 ± 0.05, 260.46 1.78, 118.69 0.71 mg/g, respectively, demonstrating increases 59.19%, 29.03%, 46.10% compared HL (HL, traditional reflux method). Furthermore, vitro digestion GLXB extract demonstrated significant lipid-lowering hypoglycemic effects. facilitated flocculation lipid droplets during exhibited capacity reduce rate fat digestion. These findings indicate that U-EAT significantly enhanced content ingredients improved activities extract, highlighting its potential further development application foods.
Язык: Английский
Процитировано
1
Journal of Molecular Structure, Год журнала: 2023, Номер 1295, С. 136598 - 136598
Опубликована: Сен. 6, 2023
Язык: Английский
Процитировано
17
Industrial Crops and Products, Год журнала: 2024, Номер 222, С. 119638 - 119638
Опубликована: Сен. 13, 2024
Язык: Английский
Процитировано
6
International Journal of Biological Macromolecules, Год журнала: 2024, Номер 268, С. 129220 - 129220
Опубликована: Янв. 6, 2024
Язык: Английский
Процитировано
5
International Journal of Biological Macromolecules, Год журнала: 2024, Номер 282, С. 137155 - 137155
Опубликована: Ноя. 4, 2024
Язык: Английский
Процитировано
5
Chemical and Biological Technologies in Agriculture, Год журнала: 2023, Номер 10(1)
Опубликована: Авг. 10, 2023
Abstract In recent years, the biological activity of plant polysaccharides has attracted more and attention. Juglans regia was one four-dry fruits in world, it very high value nutrition health care high. To better develop nutritional effects J. shell polysaccharide, used as raw material to prepare polysaccharide by water extraction alcohol precipitation method. The molecular weight, monosaccharide components, structure characteristics antioxidant activities its derivatives were studied. Then, a series meaningful data information for study obtained. weight 9.45 × 10 4 Da after separation purification DEAE-52 Sephadex G-100. contained rhamnose, arabinose, galactose, glucose, xylose galacturonic acid, molar ratio each component 6.7%: 16.5%: 28.3%: 11.2%: 12.5%: 24.8%. contains β-glucoside bond, may be → 4)- α -D-Gal p -(1 β -D-GalAp-(1 -D-Xyl glycosidic bond). activity, showed good activities, especially phosphorylated polysaccharide. important practical significance improving quality efficiency industry chain. Graphical
Язык: Английский
Процитировано
12
Molecules, Год журнала: 2024, Номер 29(3), С. 684 - 684
Опубликована: Фев. 1, 2024
In order to efficiently extract β-glucan from highland barley (HBG) and study its structural characterization antioxidant activity, ultrasonic–microwave-assisted extraction (UME) was optimized by the response surface method (RSM). Under optimal conditions of 25.05 mL/g liquid–solid ratio, 20 min ultrasonic time, 480 W microwave intensity, DPPH radical scavenging activity HBG reached 25.67%. Two polysaccharide fractions were purified HBG, namely HBG-1 HBG-2. Structural indicated that HBG-2 had similar functional groups, glycosidic linkages, linear complex chain conformation. mainly composed glucose (98.97%), while primarily consisted arabinose (38.23%), galactose (22.01%), xylose (31.60%). The molecular weight much smaller than Both exhibited concentration-dependent more active. This provided insights into efficient further investigated structure activities components Meanwhile, results this imply has potential be an in foods cosmetics.
Язык: Английский
Процитировано
4
Marine Drugs, Год журнала: 2024, Номер 22(8), С. 356 - 356
Опубликована: Авг. 2, 2024
Chlamydomonas reinhardtii polysaccharides (CRPs) are bioactive compounds derived from C. reinhardtii, yet their potential in cancer therapy remains largely unexplored. This study optimized the ultrasound-assisted extraction conditions using response surface methodology and proceeded with isolation purification of these polysaccharides. The optimal were identified as a sodium hydroxide concentration 1.5%, ultrasonic power 200 W, solid-to-liquid ratio 1:25 g/mL, an treatment time 10 min, water bath duration 2.5 h, yielding actual rate 5.71 ± 0.001%, which closely aligns predicted value 5.639%. Infrared analysis revealed that CRP-1 CRP-2 α-pyranose structures containing furoic acid, while CRP-3 CRP-4 β-pyranose acid. Experimental results demonstrated all four purified inhibited proliferation cervical (HeLa) hepatoma (HepG-2) colon (HCT-116) cells, showing most significant inhibitory effect on cancer, achieving inhibition rates 60.58 0.88% 40.44 1.44%, respectively, significantly reducing migration HeLa cells. DAPI staining confirmed inhibit cell by inducing apoptosis has liver not only elucidates application but also provides scientific basis for further development utilization.
Язык: Английский
Процитировано
4
Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Сен. 27, 2024
Язык: Английский
Процитировано
4