Ultrasonics Sonochemistry,
Год журнала:
2025,
Номер
119, С. 107396 - 107396
Опубликована: Май 23, 2025
Coptidis
Rhizoma
(CR),
a
traditional
Chinese
medicine,
has
extensive
pharmacological
activity
because
it
is
rich
in
isoquinoline
alkaloids.
Nonetheless,
the
limited
solubility
of
these
alkaloids
presents
significant
challenge,
hindering
full
realization
CR's
therapeutic
potential
through
conventional
extraction
methods.
To
address
this
issue
and
enhance
target
compounds
while
optimizing
efficiency,
study
employed
an
environmentally
sustainable
technique,
Ultrasound-Enzyme-Trehalose
Lipid
(UET),
to
synergistically
extract
five
key
from
CR.
Based
on
results
single-factor
experiments,
Box-Behnken
design
(BBD)
was
optimize
selected
model
variables.
The
optimal
conditions
were
determined:
pH
4.3,
temperature
40
℃,
solid-liquid
ratio
1:27
g/mL,
ultrasonication
time
48
min.
UET
extracts
compared
with
other
extracts,
proved
that
had
higher
alkaloid
rate,
comprehensive
evaluation
value
(CEV)
lower
energy
consumption
CO2
emission.
Then
scanning
electron
microscopy
(SEM),
dynamic
light
scattering
(DLS)
transmission
(TEM)
used
explore
mechanism
extraction.
In
addition,
more
excellent
anti-inflammatory
activity.
establishment
method
CR
provides
reference
for
green
efficient
components
natural
drugs.
Food Science & Nutrition,
Год журнала:
2024,
Номер
12(10), С. 8419 - 8431
Опубликована: Сен. 8, 2024
Abstract
Coix
seed
is
recognized
as
a
functional
medicinal
food
due
to
its
valuable
biological
activities,
with
polysaccharides
being
the
primary
active
compounds.
In
this
study,
an
ultrasonic‐assisted
enzymatic
extraction
technique
was
employed,
and
response
surface
methodology
used
optimize
yield
of
9.55
±
0.26%.
A
novel
neutral
polysaccharide,
CSPsN‐1,
purified
molecular
weight
7.75
kDa.
CSPsN‐1
composed
arabinose,
galactose,
glucose,
xylose,
mannose
in
molar
ratios
0.48:
7.92:
86.39:
2.42:
2.79.
Its
backbone
→4)‐α‐D‐Glc
p
‐(1→
→3,4)‐α‐D‐Glc
units,
terminal
residues
α‐D‐Glc
.
vitro
experiments,
enhanced
glucose
consumption
insulin‐resistant
HepG2
cells
upregulated
GLUT4
expression
by
activating
PI3K/AKT
signaling
pathway.
These
findings
suggest
that
holds
significant
promise
ingredient
for
treating
insulin
resistance
related
metabolic
disorders.
