Background:
COVID-19
was
initially
identified
as
a
respiratory
system
disorder,
but
it
has
been
discovered
to
interact
with
and
influence
the
cardiovascular
system.
COVID-19-associated
(CV)
complications
are
common,
resulting
in
high
acute
phase
mortality
large
number
of
morbidities
chronic
phase,
thus
severely
impacting
patients'
quality
life
health
outcomes.
Nevertheless,
clinical,
cellular,
molecular
biological
factors
underlying
pathophysiology
associated
poorly
understood.
Objective:
This
review
investigates
putative
clinical
well
cellular
mechanisms
by
which
leads
CV
complications,
including
state-of-the-art
genomic
sequencing-based
findings,
assessing
long-term
consequences
COVID-19,
aiming
shed
light
on
developing
strategies
for
differential
diagnosis,
risk
prognostic
stratification,
prevention,
management
sequlea
patients.
Methods:
For
this
purpose,
through
literature
published
data
carried
out
from
first
report
till
October
2023
find
comprehensive
account
genetic
diseases.
Results:
We
found
that
relationship
between
is
complex
multifaceted.
In
addition
detertriuos
effects,
survivors
may
experience
effects.
provide
detailed
involvement
alterations,
microRNAs,
novel
viral
host
proteins
CVDs
helped
identify
some
drug
targets
treat
COVID-19-related
complications.
Conclusions:
The
While
primarily
affects
system,
can
also
significantly
affect
health.
compared
classical
diseases,
there
new
related
need
specific
diagnostic
assays,
stratification
treatment
modules.
Therefore,
specail
care
must
taken
diseases
COVID-19.
Cell Proliferation,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 20, 2025
ABSTRACT
Infectious
diseases
have
become
significant
events
that
threaten
global
public
health
and
economic
development.
Since
the
20th
century,
multiple
outbreaks
of
infectious
gradually
deepened
humanity's
understanding
viral
infections,
prevention
treatment.
Organoids
possess
a
high
degree
similarity
to
human
physiological
states
strong
self‐organising
capabilities.
Research
on
based
organoids
offers
advantages
in
terms
availability,
editability
diversity.
In
this
perspective,
we
briefly
introduce
development
organoids,
focusing
historically
caused
fatal
harm
health,
such
as
HIV,
ZIKV,
SARS‐CoV‐2
MPXV.
We
further
summarise
relevant
research
pathogenic
mechanisms
these
viruses
organoid
models,
host
reactivity,
therapeutic
strategies.
Finally,
list
latest
techniques
combined
with
discuss
challenges
faced
look
forward
future
prospects
vaccine
drug
Regenerative medicine and dentistry.,
Год журнала:
2025,
Номер
unknown, С. 5 - 5
Опубликована: Март 21, 2025
Perspective
hiPSC-Driven
Organoid
Construction
and
Application
Prospects
Bangheng
Liu
1,2,
Yulei
Mu
2,3
Dong-An
Wang
1,2,*
1
Department
of
Biomedical
Engineering,
Chinese
University
Hong
Kong,
Sha
Tin,
New
Territories,
Kong
SAR
999077,
China
2
Center
for
Neuromusculoskeletal
Restorative
Medicine,
InnoHK,
HKSTP,
3
City
83
Tat
Chee
Avenue,
Kowloon,
*
Correspondence:
[email protected]
Received:
5
March
2025;
Revised:
19
Accepted:
20
Published:
21
2025
Abstract:
Induced
pluripotent
stem
cell
(iPSC)-derived
organoid
platforms
can
simulate
various
target
tissues
hold
broad
application
prospects
in
personalized
medicine,
disease
modeling,
drug
screening,
organ
transplantation,
understanding
development
mechanisms.
Currently,
the
human
iPSC
(hiPSC)
organoids
is
gradually
shifting
towards
Matrigel-free
scaffold-free
systems,
promoting
precise
control
over
composition
structure
these
systems
establishing
induction
protocols
specialized
organoids.
Researchers
are
also
exploring
construction
multifunctional
with
complex
structures
material
exchange
channels
through
vascularization,
segmented
induction,
assembly
technologies,
though
further
breakthroughs
needed.
In
future,
hiPSC
expected
to
advance
precision
treatment,
high-throughput
module
detection
multi-organ
integration,
automation.
Additionally,
when
combined
large
artificial
intelligence
models,
there
potential
establish
data
medical
platforms,
providing
support
clinical
decision-making.
Moreover,
AI
anticipated
foster
collaboration
rather
than
competition,
coordinated
growth
field.
For
hiPSC-derived
it
crucial
enhance
ethical
review
framework
balance
radical
scientific
exploration
conservative
public
attitudes.
must
optimize
or
develop
new
reduce
genomic
instability
tumorigenic
risks,
while
avoiding
emergence
non-target
cells
insufficient
functional
maturity.
IntechOpen eBooks,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 7, 2025
The
pandemic
era
has
underscored
an
urgent
demand
for
more
human-relevant
and
ethical
models
in
viral
research.
Animal
have
been
the
primary
method
studying
infection
dynamics,
immune
responses,
potential
treatments
decades.
However,
their
limitations
accurately
simulating
human-specific
responses
raise
critical
questions
about
effectiveness
of
these
predicting
human
disease
outcomes.
Organoids—three-dimensional,
stem
cell-derived
structures
replicating
tissues’
architecture
functionality—present
a
groundbreaking
alternative.
This
review
examines
transformative
organoids
to
replace
animal
virology,
particularly
under
conditions
that
require
rapid,
precise,
ethically
sound
approaches.
With
ability
closely
mimic
tissue
environments,
enable
accurate
analysis
virus-host
interactions
predictive
drug
screening.
Beyond
advancing
scientific
precision,
significantly
reduce
concerns
logistical
challenges
associated
with
testing.
As
virology
field
pivots
toward
innovative,
human-centered
models,
stand
redefine
research
approaches,
promising
new
can
accelerate
development
effective
preventive
strategies
face
future
pandemics.
The
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
Omicron
variant
exhibits
high
transmissibility
with
a
strong
immune
escape
ability
and
causes
frequent
large-scale
global
infections
by
producing
predominant
subvariants.
Here,
using
human
upper/lower
airway
intestinal
cells,
we
examined
the
previously
dominant
BA.1-BA.5
BA.2.75
subvariants,
together
recently
emerged
XBB/BQ
lineages,
in
comparison
to
former
Delta
variant.
We
observed
tendency
for
each
virus
demonstrate
higher
growth
capability
than
Unlike
bronchial
nasal
epithelial
cells
accommodated
efficient
entry
of
certain
similar
In
contrast
Delta's
reliance
on
cell-surface
transmembrane
protease
serine
2,
all
tested
variants
depended
endosomal
cathepsin
L.
Moreover,
S1/S2
cleavage
early
spikes
was
less
efficient,
whereas
recent
viruses
exhibit
improved
efficacy.
Our
results
show
that
progressively
adapts
through
continuous
endosome-mediated
host
cell
entry.IMPORTANCESARS-CoV-2,
causative
agent
disease
2019,
has
evolved
into
number
variants/subvariants,
which
have
generated
multiple
waves
infection.
order
monitor/predict
virological
features
emerging
determine
appropriate
strategies
anti-viral
development,
understanding
conserved
or
altered
evolving
SARS-CoV-2
is
important.
this
study,
addressed
subvariants
demonstrated
gradual
adaptation
cells.
route,
from
variant,
among
Collectively,
study
revealed
both
changing
maintained
during
evolution.
Materials Today Bio,
Год журнала:
2024,
Номер
30, С. 101396 - 101396
Опубликована: Дек. 10, 2024
Organoids,
exhibiting
the
capability
to
undergo
differentiation
in
specific
vitro
growth
environments,
have
garnered
significant
attention
recent
years
due
their
capacity
recapitulate
human
organs
with
resemblant
vivo
structures
and
physiological
functions.
This
groundbreaking
technology
offers
a
unique
opportunity
study
diseases
address
limitations
of
traditional
animal
models.
Cardiovascular
(CVDs),
leading
cause
mortality
worldwide,
spurred
an
increasing
number
researchers
explore
great
potential
cardiovascular
organoids
for
research.
review
initiates
by
elaborating
on
development
manufacture
organoids,
including
cardiac
blood
vessel
organoids.
Next,
we
provide
comprehensive
overview
applications
modeling
various
disorders.
Furthermore,
shed
light
prospects
CVDs
therapy,
unfold
in-depth
discussion
current
challenges
application
understanding
treating
CVDs.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(19), С. 10690 - 10690
Опубликована: Окт. 4, 2024
Cardiovascular
diseases
are
a
major
cause
of
death
worldwide.
Advanced
in
vitro
models
can
be
the
key
stone
for
better
understanding
mechanisms
at
basis
different
pathologies,
supporting
development
novel
therapeutic
protocols.
In
particular,
implementation
induced
pluripotent
stem
cell
(iPSC)
technology
allows
generation
patient-specific
line
that
is
able
to
differentiate
several
organ-specific
subsets
while
retaining
patient
genetic
background,
thus
putting
personalized
modeling
toward
medicine.
The
design
iPSC-based
recapitulate
complexity
cardiac
environment
critical
goal.
Here,
we
review
some
published
efforts
exploit
three
dimensional
(3D)
methods
relevant
cardiomyopathies,
including
genetically
and
non-genetically
determined
cardiomyopathies
cardiotoxicity
studies.
Finally,
discuss
actual
method
limitations
future
perspectives
field.
Background:
COVID-19
was
initially
identified
as
a
respiratory
system
disorder,
but
it
has
been
discovered
to
interact
with
and
influence
the
cardiovascular
system.
COVID-19-associated
(CV)
complications
are
common,
resulting
in
high
acute
phase
mortality
large
number
of
morbidities
chronic
phase,
thus
severely
impacting
patients'
quality
life
health
outcomes.
Nevertheless,
clinical,
cellular,
molecular
biological
factors
underlying
pathophysiology
associated
poorly
understood.
Objective:
This
review
investigates
putative
clinical
well
cellular
mechanisms
by
which
leads
CV
complications,
including
state-of-the-art
genomic
sequencing-based
findings,
assessing
long-term
consequences
COVID-19,
aiming
shed
light
on
developing
strategies
for
differential
diagnosis,
risk
prognostic
stratification,
prevention,
management
sequlea
patients.
Methods:
For
this
purpose,
through
literature
published
data
carried
out
from
first
report
till
October
2023
find
comprehensive
account
genetic
diseases.
Results:
We
found
that
relationship
between
is
complex
multifaceted.
In
addition
detertriuos
effects,
survivors
may
experience
effects.
provide
detailed
involvement
alterations,
microRNAs,
novel
viral
host
proteins
CVDs
helped
identify
some
drug
targets
treat
COVID-19-related
complications.
Conclusions:
The
While
primarily
affects
system,
can
also
significantly
affect
health.
compared
classical
diseases,
there
new
related
need
specific
diagnostic
assays,
stratification
treatment
modules.
Therefore,
specail
care
must
taken
diseases
COVID-19.
