Multi-omics analysis deciphers intercellular communication regulating oxidative stress to promote oral squamous cell carcinoma progression DOI Creative Commons

Hongrong Zhang,

Yemei Qian,

Yang Zhang

и другие.

npj Precision Oncology, Год журнала: 2024, Номер 8(1)

Опубликована: Ноя. 21, 2024

Oral squamous cell carcinoma (OSCC) is a common malignant tumor in the head and neck, associated with high recurrence poor prognosis. We performed an integrated analysis of single-cell RNA spatial transcriptomic data from cancerous normal tissues to create comprehensive atlas epithelial cells cancer-associated fibroblasts (CAFs). Our findings show that AKR1C3+ cells, located at tumor's stromal front, exhibit significant copy number variation prognostic indicators, suggesting role invasion. also identified distinct group early-stage CAFs (named OSCC_Normal, characterized by ADH1B+, MFAP4+, PLA2G2A+) interact where OSCC_Normal may inhibit FOXO1 redox switch these via IGF1/IGF1R pathway, causing oxidative stress overload. Conversely, use ITGA6/ITGB4 receptor counteract effects promoting cancer This study unveils complex interactions within OSCC microenvironment.

Язык: Английский

POSTN+cancer-associated fibroblasts determine the efficacy of immunotherapy in hepatocellular carcinoma DOI Creative Commons
Hao Wang, Yuan Liang, Zheng Liu

и другие.

Journal for ImmunoTherapy of Cancer, Год журнала: 2024, Номер 12(7), С. e008721 - e008721

Опубликована: Июль 1, 2024

Objective Hepatocellular carcinoma (HCC) poses a significant clinical challenge because the long-term benefits of immune checkpoint blockade therapy are limited. A comprehensive understanding mechanisms underlying immunotherapy resistance in HCC is imperative for improving patient prognosis. Design In this study, to systematically investigate characteristics cancer-associated fibroblast (CAF) subsets and dynamic communication among tumor microenvironment (TME) components regulated by CAF subsets, we generated an atlas compiling single-cell RNA sequencing (scRNA-seq) datasets on 220 samples from six datasets. We combined spatial transcriptomics with scRNA-seq multiplexed immunofluorescence identify specific TME that determine efficacy patients. Results Our findings highlight pivotal role POSTN + CAFs as potent response barriers at locations, they hinder effective T-cell infiltration decrease immunotherapy. Additionally, elucidated interplay between SPP1 macrophages, whereby former recruits latter triggers increased expression via IL-6/STAT3 signaling pathway. Moreover, demonstrated correlation revealing immunosuppressive limits response. Notably, found patients elevated levels both macrophages achieved less therapeutic benefit cohort. Conclusion research elucidates light particular subset resistance, emphasizing potential targeting subpopulations improve responses

Язык: Английский

Процитировано

13
Spatial multiomics reveals a subpopulation of fibroblasts associated with cancer stemness in human hepatocellular carcinoma DOI Creative Commons
Siyu Jing, Dan Liu,

Na Feng

и другие.

Genome Medicine, Год журнала: 2024, Номер 16(1)

Опубликована: Авг. 13, 2024

Cancer-associated fibroblasts (CAFs) are the prominent cell type in tumor microenvironment (TME), and CAF subsets have been identified various tumors. However, how CAFs spatially coordinate other populations within liver TME to promote cancer progression remains unclear.

Язык: Английский

Процитировано

8
Exploring the diversity of cancer-associated fibroblasts: insights into mechanisms of drug resistance DOI Creative Commons
Anastasia N. Kazakova, Maria M. Lukina, Ksenia S. Anufrieva

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 12

Опубликована: Май 16, 2024

Introduction: Among the various stromal cell types within tumor microenvironment, cancer-associated fibroblasts (CAFs) emerge as predominant constituent, exhibiting a diverse array of oncogenic functions not intrinsic to normal fibroblasts. Their involvement spans across all stages tumorigenesis, encompassing initiation, progression, and metastasis. Current understanding posits coexistence distinct subpopulations CAFs microenvironment spectrum solid tumors, showcasing both pro- antitumor activities. Recent advancements in single-cell transcriptomics have revolutionized our ability meticulously dissect heterogeneity inherent CAF populations. Furthermore, accumulating evidence underscores pivotal role conferring therapeutic resistance tumors against drug modalities. Consequently, efforts are underway develop pharmacological agents specifically targeting CAFs. Methods: This review embarks on comprehensive analysis, consolidating data from 36 independent RNA sequencing investigations spanning 17 human malignant types. Results: Our exploration centers elucidating population markers, discerning their prognostic relevance, delineating functional contributions, underlying mechanisms orchestrating chemoresistance. Discussion: Finally, we deliberate potential harnessing promising targets for intervention strategies clinical oncology.

Язык: Английский

Процитировано

7
Current state and future prospects of spatial biology in colorectal cancer DOI Creative Commons
Francisco Carranza, Fernando C. Diaz, Maria Ninova

и другие.

Frontiers in Oncology, Год журнала: 2024, Номер 14

Опубликована: Дек. 3, 2024

Over the past century, colorectal cancer (CRC) has become one of most devastating cancers impacting human population. To gain a deeper understanding molecular mechanisms driving this solid tumor, researchers have increasingly turned their attention to tumor microenvironment (TME). Spatial transcriptomics and proteomics emerged as particularly powerful technology for deciphering complexity CRC tumors, given that TME its spatial organization are critical determinants disease progression treatment response. enables high-resolution mapping whole transcriptome. While maps protein expression function across tissue sections. Together, they provide detailed view landscape cellular interactions within TME. In review, we delve into recent advances in biology technologies applied research, highlighting both methodologies challenges associated with use, such substantial heterogeneity characteristic CRC. We also discuss limitations current approaches need novel computational tools manage interpret these complex datasets. conclude, emphasize importance further developing integrating precision medicine strategies enhance therapeutic targeting improve patient outcomes.

Язык: Английский

Процитировано

6
Innovations in Short-Read Sequencing Technologies and Their Applications to Clinical Genomics DOI
Katarzyna Polonis, Joseph H. Blommel, Andrew Hughes

и другие.

Clinical Chemistry, Год журнала: 2025, Номер 71(1), С. 97 - 108

Опубликована: Янв. 1, 2025

Massively parallel sequencing (MPS) of nucleic acids has been a transformative technology for basic and applied genomic science, increasing efficiencies decreasing costs to enable studies unprecedented scope impact. In clinical settings, these technological scientific advances have led the development tests that are increasingly fast, comprehensive, more frequently employed. Practitioners medicine tools across including diagnosis inherited disorders cancers infectious disease detection surveillance. recent years, commercial marketplace MPS sequencers reagents dominated by few companies. The growing demand emergence several new platforms with techniques may provide alternatives or improvements existing workflows allow adoption in settings. Clinical genomics laboratories will evaluate from unique perspective, focusing on how advancements can improve patient care.

Язык: Английский

Процитировано

0
Progress in research of local regrowth after neoadjuvant therapy in rectal cancer DOI

Xing-Mei Yuan,

Qin-Xing Cao,

Li Yan

и другие.

Shijie huaren xiaohua zazhi, Год журнала: 2025, Номер 33(1), С. 43 - 50

Опубликована: Янв. 22, 2025

Язык: Английский

Процитировано

0
Progress in research of local regrowth after neoadjuvant therapy in rectal cancer DOI

Xing-Mei Yuan,

Qin-Xing Cao,

Li Yan

и другие.

Shijie huaren xiaohua zazhi, Год журнала: 2025, Номер 33(1), С. 37 - 44

Опубликована: Янв. 25, 2025

Язык: Английский

Процитировано

0
CXCL12 as a Potential Hub Gene for N-Acetylcysteine Treatment of T1DM Liver Disease DOI Creative Commons

Menglong Zhao,

Mingzheng Han,

Sen Guo

и другие.

Biomolecules, Год журнала: 2025, Номер 15(2), С. 176 - 176

Опубликована: Янв. 25, 2025

The etiology of type 1 diabetes mellitus (T1DM) is intricate, leading to its classification as an autoimmune metabolic disorder. T1DM often coexists with various visceral diseases. N-acetylcysteine (NAC) widely acknowledged for potent antioxidant properties. Studies have demonstrated that the combination NAC and insulin can effectively alleviate iron-induced nephropathy in mitigate oxidative stress injury skeletal muscle associated condition. However, potential impact alone on liver disease individuals remains uncertain. In this study, a beagle model was established simulate T1DM, enabling investigation into role using RNA-seq biogenic analysis subsequent validation through molecular biological methods. findings revealed suppressed expression CXCL12 chemokine livers while treatment induced specific activation within affected by T1DM. These results suggest may serve regulatory factor involved therapeutic effects TIDM. This discovery holds significant implications utilizing adjunctive therapy managing complicated diseases accompanying mellitus.

Язык: Английский

Процитировано

0
Collagen remodeling-mediated signaling pathways and their impact on tumor therapy DOI Creative Commons

Zihang Yuan,

Bo Lin, Chunlan Wang

и другие.

Journal of Biological Chemistry, Год журнала: 2025, Номер unknown, С. 108330 - 108330

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Neoadjuvant therapy: Dawn of reducing the high post-surgery recurrence rate of hepatocellular carcinoma DOI
Xiaodong Zhang,

Luyi Zhang,

Jialiang Luo

и другие.

World Journal of Gastrointestinal Surgery, Год журнала: 2025, Номер 17(3)

Опубликована: Фев. 24, 2025

The high postoperative recurrence rate remains a major challenge in the treatment of hepatocellular carcinoma (HCC) following resection. Increasing research has been delved into investigating role neoadjuvant therapy on prognosis resectable HCC. Recent trends combination with molecularly targeted agents and immune checkpoint inhibitors have significantly improved efficacy systemic antitumor treatments, yielding survival benefits exceeding 40%. Neoadjuvant for HCC, whether based locoregional therapies, or their combination, emerged as promising direction. However, there matter debate therapy. In this review, we summarize discuss progress challenges HCC over past five years from perspective Chinese guidelines to provide new insights future directions field.

Язык: Английский

Процитировано

0